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AB236655

Anti-CAMLG/CAML antibody

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Rabbit Polyclonal CAMLG/CAML antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CAMLG aa 1-200.

View Alternative Names

CAML, GET2, CAMLG, Guided entry of tail-anchored proteins factor CAMLG, Calcium signal-modulating cyclophilin ligand

3 Images
Western blot - Anti-CAMLG/CAML antibody (AB236655)
  • WB

Supplier Data

Western blot - Anti-CAMLG/CAML antibody (AB236655)

All lanes:

Western blot - Anti-CAMLG/CAML antibody (ab236655) at 1/500 dilution

Lane 1:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

Lane 2:

HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate

Lane 3:

U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate

Lane 4:

SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 33 kDa

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Immunocytochemistry/ Immunofluorescence - Anti-CAMLG/CAML antibody (AB236655)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CAMLG/CAML antibody (AB236655)

HepG2 (human liver hepatocellular carcinoma cell line) cells stained for CAMLG/CAML (green) using ab236655 at 1/100 dilution in ICC/IF. Secondary antibody is Alexa Fluor® 488-congugated Goat Anti-Rabbit IgG (H+L).

Immunoprecipitation - Anti-CAMLG/CAML antibody (AB236655)
  • IP

Supplier Data

Immunoprecipitation - Anti-CAMLG/CAML antibody (AB236655)

CAMLG/CAML was immunoprecipitated from 500 μg HEK-293 (human epithelial cell line from embryonic kidney) whole cell extract with ab236655 at 1/200 dilution.

Lane 1 : Control rabbit IgG (1 μg) IP in HEK-293 whole cell lysate.

Lane 2 : ab236655 IP in HEK-293 whole cell lysate.

Lane 3 : HEK-293 whole cell lysate (10 μg) Input.

HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.

All lanes:

Immunoprecipitation - Anti-CAMLG/CAML antibody (ab236655)

Predicted band size: 33 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human CAMLG aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

P49069

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/200 - 1/2000", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/500", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CAMLG also known as CAML (calcium modulating ligand) is a protein with a molecular mass of approximately 37 kDa. CAMLG has an essential role in cellular calcium signaling processes. It is predominantly expressed in the endoplasmic reticulum membrane in various cell types. CAMLG's function involves mediating calcium influx and maintenance which affects multiple cellular operations. The protein interacts with membrane-bound elements facilitating its role in signaling and cellular homeostasis.
Biological function summary

CAMLG contributes to proper cellular signaling and function by managing calcium flux. It is part of larger protein complexes that regulate calcium-dependent cellular activities. CAMLG assists in the modulation of intracellular calcium levels that influence processes like cell proliferation differentiation and apoptosis. As part of its complex interactions CAMLG collaborates with other proteins involved in calcium signal transduction.

Pathways

Cellular calcium homeostasis mechanisms feature CAMLG as a critical component. This protein functions within pathways such as the NFAT (nuclear factor of activated T-cells) signaling and IP3 (inositol 145-trisphosphate) pathway. In these systems CAMLG interacts with other proteins like IP3 receptor affecting the release and regulation of calcium ions which impact gene transcription and immune response.

Dysfunction in CAMLG activity relates to immune system irregularities and cancer. Disruption in calcium signaling pathways implicates CAMLG in conditions like autoimmune disorders affecting immune cell regulation. Abnormal expression or mutations in CAMLG may also contribute to cancerous cell proliferation when linked to proteins such as NFAT emphasizing its involvement in oncogenic pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum (PubMed : 23041287, PubMed : 24392163, PubMed : 27226539). Together with GET1/WRB, acts as a membrane receptor for soluble GET3/TRC40, which recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol (PubMed : 23041287, PubMed : 24392163, PubMed : 27226539). Required for the stability of GET1 (PubMed : 32187542). Stimulates calcium signaling in T cells through its involvement in elevation of intracellular calcium (PubMed : 7522304). Essential for the survival of peripheral follicular B cells (By similarity).
See full target information CAMLG
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Product promise

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