AB134901

Anti-cAMP antibody [EP8471]

Name: Anti-cAMP antibody [EP8471] Rabbit monoclonal (ab134901) | Abcam
Brand: Abcam Limited
SKU: AB134901
Price: 207 USD
Availability: InStock
Rating: 5 (2 reviews)

20ul selling size
RabMAb
Recombinant
What is this?

(2 Reviews)

(9 Publications )

Rabbit Recombinant Monoclonal cAMP antibody. Suitable for ELISA and reacts with Chemical samples. Cited in 9 publications.

1 Images

ELISA - Anti-cAMP antibody [EP8471] (AB134901)

ELISA

Lab

ELISA - Anti-cAMP antibody [EP8471] (AB134901)

Competitive ELISA antigen dose-response graph using purified ab134901 (0.25 µg/mL). Antigen concentration of 0.1 µg/mL. 0.1 μg/mL antigen was coated. Free cAMP and other competitors (2.5 mg/mL) were added respectively. AffiniPure Goat Anti-Rabbit IgG FC-HRP (1/2500 dilution) was used as the secondary antibody.

Carrier free

Anti-cAMP antibody [EP8471] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP8471

Isotype

IgG

Carrier free

Applications

ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

<p>This antibody does not cross react with cGMP, GMP, GDP, cIMP, cCMP, or adenosine.</p>

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "0.25 mg/mL", "ELISA-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cyclic adenosine monophosphate (cAMP) serves as an essential secondary messenger in various signal transduction pathways. Known also by its chemical abbreviation cAMP this small molecule has a mass of approximately 329.21 g/mol. cAMP is synthesized from ATP by the enzyme adenylate cyclase which is present in many cell types notably in neurons and various endocrine tissues. cAMP acts by activating protein kinase A (PKA) which then phosphorylates target proteins to alter cellular function.

Biological function summary

CAMP regulates numerous cellular processes including metabolism gene expression and cell growth. It functions as an important part of G protein-coupled receptor (GPCR) signaling pathways where its concentration can significantly change influenced by external signals. cAMP modulates the activity of ion channels and various enzymes often participating in feedback loops to maintain cellular homeostasis.

Pathways

CAMP integrates into critical signal transduction pathways such as the cyclic AMP-dependent pathway and the calcium signaling pathway. It works closely with proteins like CREB (cAMP response element-binding protein) to regulate gene transcription. cAMP also impacts other molecules like phosphodiesterases which break it down maintaining its transient nature in response to stimuli.

Irregular cAMP levels associate with pathologies like heart failure and certain types of cancer. Disruptions in cAMP signaling can lead to abnormal cell growth or inadequate cellular response to hormonal signals. Through its interaction with proteins like CFTR (cystic fibrosis transmembrane conductance regulator) cAMP influences disorders such as cystic fibrosis highlighting its critical role in maintaining cellular function and signaling balance.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information cAMP

Alternative Names

3'-5'-cyclic adenosine monophosphate, Adenosine 3' 5' cyclic monophosphate, Adenosine 3閳 5閳 cyclic monophosphate, Cyclic adenosine monophosphate

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Publications (9)

Recent publications for all applications. Explore the full listand refine your search

International journal of molecular sciences 25: PubMed39126032

2024

Anti-Inflammatory Effects of miR-369-3p via PDE4B in Intestinal Inflammatory Response.

Applications

Unspecified application

Species

Unspecified reactive species

Viviana Scalavino,Emanuele Piccinno,Nicoletta Labarile,Raffaele Armentano,Gianluigi Giannelli,Grazia Serino

Journal of cellular and molecular medicine 28:e18366 PubMed38856956

2024

Overexpression of olfactory receptor 78 ameliorates brain injury in cerebral ischaemia-reperfusion rats by activating Prkaca-mediated cAMP/PKA-MAPK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Kang,Lijuan Zhu,Yanli Xue,Qian Yang,Qi Lei,Qianqian Wang

Frontiers in pharmacology 15:1371929 PubMed38576483

2024

Xiasangju alleviate metabolic syndrome by enhancing noradrenaline biosynthesis and activating brown adipose tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Changhao He,Yongcheng An,Lu Shi,Yan Huang,Huilin Zhang,Wanxin Fu,Menglu Wang,Ziyi Shan,Yuhang Du,Jiamei Xie,Zhiyun Huang,Weiguang Sun,Yonghua Zhao,Baosheng Zhao

eLife 12: PubMed37960975

2023

Astrocyte and L-lactate in the anterior cingulate cortex modulate schema memory and neuronal mitochondrial biogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Mastura Akter,Mahadi Hasan,Aruna Surendran Ramkrishnan,Zafar Iqbal,Xianlin Zheng,Zhongqi Fu,Zhuogui Lei,Anwarul Karim,Ying Li

Nature 622:383-392 PubMed37731001

2023

The β-adrenergic receptor links sympathetic nerves to T cell exhaustion.

Applications

Unspecified application

Species

Unspecified reactive species

Anna-Maria Globig,Steven Zhao,Jessica Roginsky,Vivien I Maltez,Juan Guiza,Natalia Avina-Ochoa,Maximilian Heeg,Filipe Araujo Hoffmann,Omkar Chaudhary,Jiawei Wang,Gokhan Senturk,Dan Chen,Carolyn O'Connor,Samuel Pfaff,Ronald N Germain,Kurt A Schalper,Brinda Emu,Susan M Kaech

Proceedings of the National Academy of Sciences of the United States of America 120:e2304168120 PubMed37603749

2023

Gap junction-transported cAMP from the niche controls stem cell progeny differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Renjun Tu,Xiaohan Alex Tang,Rui Xu,Zhaohua Ping,Zulin Yu,Ting Xie

International journal of nanomedicine 17:1549-1566 PubMed35401001

2022

Pseudoephedrine Nanoparticles Alleviate Adriamycin-Induced Reproductive Toxicity Through the GnRhR Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Fu,Peipei Yuan,Yajuan Zheng,Yaxin Wei,Liyuan Gao,Yuan Ruan,Yi Chen,Panying Li,Weisheng Feng,Xiaoke Zheng

Autophagy 16:2193-2205 PubMed32003282

2020

Microglial autophagy defect causes parkinson disease-like symptoms by accelerating inflammasome activation in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jinbo Cheng,Yajin Liao,Yuan Dong,Han Hu,Nannan Yang,Xiangxi Kong,Shuoshuo Li,Xiaoheng Li,Jifeng Guo,Lixia Qin,Jiezhong Yu,Cungen Ma,Jianke Li,Mingtao Li,Beisha Tang,Zengqiang Yuan

Molecular medicine reports 20:2143-2150 PubMed31322181

2019

Pinoresinol promotes MC3T3‑E1 cell proliferation and differentiation via the cyclic AMP/protein kinase A signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Jiang,Wenjing Chen,Fuguo Shen,Wenlong Xiao,Hongliang Guo,Hang Su,Jiang Xiu,Wencai Sun

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-cAMP antibody [EP8471]

ELISA - Anti-cAMP antibody [EP8471] (AB134901)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Applications

Immunogen

Specificity

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Assay Kits

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Alternative Names

Publications (9)

Product promise