Anti-cAMP Protein Kinase Catalytic subunit antibody [EP2102Y] - BSA and Azide free

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-cAMP Protein Kinase Catalytic subunit antibody [EP2102Y] - BSA and Azide free (AB235385)

Overlay histogram showing HeLa cells stained with ab76238 (unpurified) (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76238, 1/100 dilution) for 30 min at 22^oC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22^oC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76238).

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-cAMP Protein Kinase Catalytic subunit antibody [EP2102Y] - BSA and Azide free (AB235385)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling cAMP Protein Kinase Catalytic subunit with purified ab76238 at 1 : 100 dilution (8.0 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (anti camp protein kinase catalytic subunit antibody ep2102y immunocytochemistry hela human)

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-cAMP Protein Kinase Catalytic subunit antibody [EP2102Y] - BSA and Azide free (AB235385)

Intracellular Flow Cytometry analysis of MCF-7 (Human breast adenocarcinoma epithelial cell) cells labeling cAMP Protein Kinase Catalytic subunit with purified ab76238 at 1/80 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76238)

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-cAMP Protein Kinase Catalytic subunit antibody [EP2102Y] - BSA and Azide free (AB235385)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid carcinoma tissue sections labeling cAMP Protein Kinase Catalytic subunit with purified ab76238 at 1/750 dilution. Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76238)

• IP

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Immunoprecipitation - Anti-cAMP Protein Kinase Catalytic subunit antibody [EP2102Y] - BSA and Azide free (AB235385)

ab76238 (purified) at 1/40 dilution (2 µg) immunoprecipitating cAMP Protein Kinase Catalytic subunit in MCF-7 whole cell lysate.
Lane 1 (input) : MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysate 10 µg
Lane 2 (+) : ab76238 & MCF-7 whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab76238 in MCF-7 whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76238)

All lanes:

Immunoprecipitation - Anti-cAMP Protein Kinase Catalytic subunit antibody [EP2102Y] (<a href='/en-us/products/primary-antibodies/camp-protein-kinase-catalytic-subunit-antibody-ep2102y-ab76238'>ab76238</a>)

Predicted band size: 41 kDa

false

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-cAMP Protein Kinase Catalytic subunit antibody [EP2102Y] - BSA and Azide free (AB235385)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebrum tissue sections labeling cAMP Protein Kinase Catalytic subunit with purified ab76238 at 1/750 dilution. Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76238)

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-cAMP Protein Kinase Catalytic subunit antibody [EP2102Y] - BSA and Azide free (AB235385)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat stomach tissue sections labeling cAMP Protein Kinase Catalytic subunit with purified ab76238 at 1/750 dilution. Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76238)