AB251766

Anti-CAMSAP2 antibody

Name: Anti-CAMSAP2 antibody (ab251766) | Abcam
Brand: Abcam Limited
SKU: AB251766
Price: 655 USD
Availability: InStock

Be the first to review this product! Submit a review

(0 Publication)

Rabbit Polyclonal CAMSAP2 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CAMSAP2 aa 600-700.

View Alternative Names

CAMSAP1L1, KIAA1078, CAMSAP2, Calmodulin-regulated spectrin-associated protein 2, Calmodulin-regulated spectrin-associated protein 1-like protein 1

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAMSAP2 antibody (AB251766)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAMSAP2 antibody (AB251766)

Paraffin-embedded human prostate tissue stained for CAMSAP2 using ab251766 at 1/200 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-CAMSAP2 antibody (AB251766)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CAMSAP2 antibody (AB251766)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for CAMSAP2 (green) using ab251766 at 4 μg/ml in ICC/IF.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAMSAP2 antibody (AB251766)

Paraffin-embedded human testis tissue stained for CAMSAP2 using ab251766 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAMSAP2 antibody (AB251766)

Paraffin-embedded human placenta tissue stained for CAMSAP2 using ab251766 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAMSAP2 antibody (AB251766)

Paraffin-embedded human cerebral cortex tissue stained for CAMSAP2 using ab251766 at 1/200 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human CAMSAP2 aa 600-700. The exact immunogen used to generate this antibody is proprietary information.

Q08AD1

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200.00000 - 1/500.00000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25000-2.00000 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CAMSAP2 also known as calmodulin-regulated spectrin-associated protein family member 2 is a microtubule-stabilizing protein with a molecular mass of approximately 198 kDa. It binds to the minus ends of microtubules stabilizing them and regulating their dynamics. CAMSAP2 is expressed in various tissues with a significant presence in neuronal tissue. The protein plays a vital role in stabilizing the microtubule network which influences cellular architecture and intracellular transport.

Biological function summary

The regulation of microtubule dynamics by CAMSAP2 ensures proper neuronal development and function. CAMSAP2 is part of a protein complex that is essential for maintaining microtubule integrity and organization. It interacts with other proteins such as CAMSAP1 and CAMSAP3 each playing distinct roles in microtubule arrangement. These interactions help in maintaining cellular structures which are necessary for processes like cellular division and differentiation.

Pathways

CAMSAP2 is involved in microtubule-associated pathways critical for cell stability and signaling. The protein is part of the axon guidance pathway where it functions alongside other proteins including PTEN and CRMP2 to ensure correct neural network formation. CAMSAP2 also engages in the regulation of cytoskeleton organization pathways where it collaborates with microtubule-associated proteins to support cellular morphology and dynamics.

CAMSAP2 plays a part in conditions affecting the nervous system such as neurodevelopmental disorders and certain neurodegenerative diseases. Mutations or dysregulation of CAMSAP2 have been linked to intellectual disabilities due to its role in neuronal structure and signaling. This protein also interacts with tau a protein commonly associated with neurodegenerative disorders like Alzheimer's disease suggesting a potential link between CAMSAP2 function and tauopathies.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Key microtubule-organizing protein that specifically binds the minus-end of non-centrosomal microtubules and regulates their dynamics and organization (PubMed : 23169647, PubMed : 24486153, PubMed : 24706919). Specifically recognizes growing microtubule minus-ends and autonomously decorates and stabilizes microtubule lattice formed by microtubule minus-end polymerization (PubMed : 24486153, PubMed : 24706919). Acts on free microtubule minus-ends that are not capped by microtubule-nucleating proteins or other factors and protects microtubule minus-ends from depolymerization (PubMed : 24486153, PubMed : 24706919). In addition, it also reduces the velocity of microtubule polymerization (PubMed : 24486153, PubMed : 24706919). Through the microtubule cytoskeleton, also regulates the organization of cellular organelles including the Golgi and the early endosomes (PubMed : 27666745). Essential for the tethering, but not for nucleation of non-centrosomal microtubules at the Golgi : together with Golgi-associated proteins AKAP9 and PDE4DIP, required to tether non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement (PubMed : 27666745). Also acts as a regulator of neuronal polarity and development : localizes to non-centrosomal microtubule minus-ends in neurons and stabilizes non-centrosomal microtubules, which is required for neuronal polarity, axon specification and dendritic branch formation (PubMed : 24908486). Through the microtubule cytoskeleton, regulates the autophagosome transport (PubMed : 28726242).

See full target information CAMSAP2

style

left-column

style

left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

style

left-column

style

right-column

style

bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com