AB183748

Anti-CAND1 antibody [EPR14241] - N-terminal

RabMAb
Recombinant
What is this?

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(4 Publications)

Rabbit Recombinant Monoclonal CAND1 antibody. N-terminal. Suitable for IP, WB, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 4 publications.

View Alternative Names

KIAA0829, TIP120, TIP120A, CAND1, Cullin-associated NEDD8-dissociated protein 1, Cullin-associated and neddylation-dissociated protein 1, TBP-interacting protein of 120 kDa A, p120 CAND1, TBP-interacting protein 120A

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAND1 antibody [EPR14241] - N-terminal (AB183748)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAND1 antibody [EPR14241] - N-terminal (AB183748)

Immunohistochemical analysis of Paraffin-embedded Human pancreas tissue labeling CAND1 with ab183748 at 1/100 dilution. HRP conjugated Rabbit IgG was used as a secondary antibody. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-CAND1 antibody [EPR14241] - N-terminal (AB183748)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-CAND1 antibody [EPR14241] - N-terminal (AB183748)

Intracellular flow cytometric analysis of 293T cells (fixed in 2% paraformaldehyde) labeling CAND1 with ab183748at 1/220 dilution (red).

Isotype control Rabbit monoclonal IgG (green). Secondary antibody Goat anti rabbit IgG (FITC) at a 1/150 dilution.

Supplier Data

Immunoprecipitation - Anti-CAND1 antibody [EPR14241] - N-terminal (AB183748)

Western blot analysis of CAND1 in immunoprecipitation pellets from Jurkat lysate. ab183748 used at 1/50 dilution. Secondary antibody Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000.

All lanes:

Immunoprecipitation - Anti-CAND1 antibody [EPR14241] - N-terminal (ab183748)

Predicted band size: 136 kDa

false

Supplier Data

Western blot - Anti-CAND1 antibody [EPR14241] - N-terminal (AB183748)

All lanes:

Western blot - Anti-CAND1 antibody [EPR14241] - N-terminal (ab183748) at 1/20000 dilution

Lane 1:

293T whole cell lysate at 20 µg

Lane 2:

Jurkat whole cell lysate at 20 µg

Lane 3:

Saos-2 whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 136 kDa

false

Carrier free

Anti-CAND1 antibody [EPR14241] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14241

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, IHC-P, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50 - 1/70", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/220", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CAND1 also known as cullin-associated and neddylation-dissociated 1 is a scaffolding protein with a molecular mass of approximately 130 kDa. It is found in the cytoplasm and nucleus of eukaryotic cells. CAND1 plays a critical role in regulating the activity of cullin-RING ubiquitin ligases (CRLs) which are important for protein degradation processes. By interacting with neddylated cullin proteins CAND1 modulates the assembly and disassembly of these CRL complexes therefore influencing their activity.

Biological function summary

CAND1 mediates the dynamics of CRL complexes acting as a regulator of substrate specificity and turnover. CAND1 does not function alone; it is integral to a complex that includes cullin proteins and various CRL adaptors. This interaction ensures the right substrates are targeted for ubiquitination leading to proteasomal degradation. The ability of CAND1 to bind to cullin substrates is influenced by cullin neddylation and deneddylation cycles making it essential for the proper function of the ubiquitin-proteasome system.

Pathways

Studies have shown that CAND1 is important in the ubiquitin-proteasome pathway along with the neddylation pathway. Within these pathways it primarily interacts with proteins such as cullins 1 3 and 4 which are central to CRLs. By regulating these interactions CAND1 ensures that protein turnover is controlled which is important for processes like cell cycle progression and signal transduction.

Research suggests a connection between CAND1 dysregulation and certain cancers as well as neurodegenerative disorders. In cancer aberrant regulation of CAND1 can lead to uncontrolled cell proliferation due to faulty protein degradation. In neurodegenerative diseases ineffective CAND1-mediated pathways may contribute to the accumulation of damaged proteins. Understanding CAND1's relationship with key proteins in these conditions such as p53 in cancer highlights its potential as a therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Key assembly factor of SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complexes that promotes the exchange of the substrate-recognition F-box subunit in SCF complexes, thereby playing a key role in the cellular repertoire of SCF complexes. Acts as a F-box protein exchange factor. The exchange activity of CAND1 is coupled with cycles of neddylation conjugation : in the deneddylated state, cullin-binding CAND1 binds CUL1-RBX1, increasing dissociation of the SCF complex and promoting exchange of the F-box protein. Probably plays a similar role in other cullin-RING E3 ubiquitin ligase complexes.

See full target information CAND1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:3890 PubMed38719850

2024

The Shigella kinase effector OspG modulates host ubiquitin signaling to escape septin-cage entrapment.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Xian,Jiaqi Fu,Qinxin Zhang,Chuang Li,Yan-Bo Zhao,Zhiheng Tang,Yi Yuan,Ying Wang,Yan Zhou,Peter S Brzoic,Ning Zheng,Songying Ouyang,Zhao-Qing Luo,Xiaoyun Liu

Cellular & molecular biology letters 29:36 PubMed38486141

2024

Obesity-induced downregulation of miR-192 exacerbates lipopolysaccharide-induced acute lung injury by promoting macrophage activation.

Applications

Unspecified application

Species

Unspecified reactive species

Siqi Wu,Wenjing Tang,Ling Liu,Ke Wei,Yin Tang,Jingyue Ma,Hongbin Li,Yichan Ao

Clinical and translational medicine 13:e1443 PubMed37837399

2023

Cullin-associated and neddylation-dissociated 1 regulate reprogramming of lipid metabolism through SKP1-Cullin-1-F-box -mediated heterogeneous nuclear ribonucleoprotein A2/B1 ubiquitination and promote hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Zhang,Peng Xia,Zhangshuo Yang,Jie Liu,Yimin Zhu,Zan Huang,Zhonglin Zhang,Yufeng Yuan

American journal of physiology. Renal physiology 324:F315-F328 PubMed36727946

2023

Ubiquitination of NKCC2 by the cullin-RING E3 ubiquitin ligase family in the thick ascending limb of the loop of Henle.

Applications

Unspecified application

Species

Unspecified reactive species

Gustavo R Ares

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CAND1 antibody [EPR14241] - N-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAND1 antibody [EPR14241] - N-terminal (AB183748)

Flow Cytometry (Intracellular) - Anti-CAND1 antibody [EPR14241] - N-terminal (AB183748)

Immunoprecipitation - Anti-CAND1 antibody [EPR14241] - N-terminal (AB183748)

Western blot - Anti-CAND1 antibody [EPR14241] - N-terminal (AB183748)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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