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Rabbit Recombinant Monoclonal CAND1 antibody. C-terminal. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples.


Images

Immunocytochemistry/ Immunofluorescence - Anti-CAND1 antibody [EPR14242(B)] - C-terminal (AB181216), expandable thumbnail
  • Western blot - Anti-CAND1 antibody [EPR14242(B)] - C-terminal (AB181216), expandable thumbnail
  • Immunoprecipitation - Anti-CAND1 antibody [EPR14242(B)] - C-terminal (AB181216), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAND1 antibody [EPR14242(B)] - C-terminal (AB181216), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-CAND1 antibody [EPR14242(B)] - C-terminal (AB181216), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Predicted
Predicted
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/40 - 1/60

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/10000 - 1/50000

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100 - 1/250

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/20

Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/50 - 1/100

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Key assembly factor of SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complexes that promotes the exchange of the substrate-recognition F-box subunit in SCF complexes, thereby playing a key role in the cellular repertoire of SCF complexes. Acts as a F-box protein exchange factor. The exchange activity of CAND1 is coupled with cycles of neddylation conjugation: in the deneddylated state, cullin-binding CAND1 binds CUL1-RBX1, increasing dissociation of the SCF complex and promoting exchange of the F-box protein. Probably plays a similar role in other cullin-RING E3 ubiquitin ligase complexes.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal CAND1 antibody. C-terminal. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR14242(B)

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CAND1 also known as cullin-associated and neddylation-dissociated 1 is a scaffolding protein with a molecular mass of approximately 130 kDa. It is found in the cytoplasm and nucleus of eukaryotic cells. CAND1 plays a critical role in regulating the activity of cullin-RING ubiquitin ligases (CRLs) which are important for protein degradation processes. By interacting with neddylated cullin proteins CAND1 modulates the assembly and disassembly of these CRL complexes therefore influencing their activity.

Biological function summary

CAND1 mediates the dynamics of CRL complexes acting as a regulator of substrate specificity and turnover. CAND1 does not function alone; it is integral to a complex that includes cullin proteins and various CRL adaptors. This interaction ensures the right substrates are targeted for ubiquitination leading to proteasomal degradation. The ability of CAND1 to bind to cullin substrates is influenced by cullin neddylation and deneddylation cycles making it essential for the proper function of the ubiquitin-proteasome system.

Pathways

Studies have shown that CAND1 is important in the ubiquitin-proteasome pathway along with the neddylation pathway. Within these pathways it primarily interacts with proteins such as cullins 1 3 and 4 which are central to CRLs. By regulating these interactions CAND1 ensures that protein turnover is controlled which is important for processes like cell cycle progression and signal transduction.

Associated diseases and disorders

Research suggests a connection between CAND1 dysregulation and certain cancers as well as neurodegenerative disorders. In cancer aberrant regulation of CAND1 can lead to uncontrolled cell proliferation due to faulty protein degradation. In neurodegenerative diseases ineffective CAND1-mediated pathways may contribute to the accumulation of damaged proteins. Understanding CAND1's relationship with key proteins in these conditions such as p53 in cancer highlights its potential as a therapeutic target.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-CAND1 antibody [EPR14242(B)] - C-terminal (ab181216), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CAND1 antibody [EPR14242(B)] - C-terminal (ab181216)

    Immunofluorescent analysis of HeLa cells (-20°C Acetone-fixed) labeling CAND1 with ab181216 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 dilution and counterstained with Dapi (blue).

  • Western blot - Anti-CAND1 antibody [EPR14242(B)] - C-terminal (ab181216), expandable thumbnail

    Western blot - Anti-CAND1 antibody [EPR14242(B)] - C-terminal (ab181216)

    All lanes: Western blot - Anti-CAND1 antibody [EPR14242(B)] - C-terminal (ab181216) at 1/50000 dilution

    Lane 1: 293T cell lysate at 20 µg

    Lane 2: HeLa cell lysate at 20 µg

    Lane 3: Jurkat cell lysate at 20 µg

    Lane 4: Saos-2 cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 136 kDa

  • Immunoprecipitation - Anti-CAND1 antibody [EPR14242(B)] - C-terminal (ab181216), expandable thumbnail

    Immunoprecipitation - Anti-CAND1 antibody [EPR14242(B)] - C-terminal (ab181216)

    Western blot analysis of immunoprecipitation pellet from Hela cell lysate immunoprecipitated using ab181216 at 1/50 dilution.
    Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

    All lanes: Immunoprecipitation - Anti-CAND1 antibody [EPR14242(B)] - C-terminal (ab181216)

    Predicted band size: 136 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAND1 antibody [EPR14242(B)] - C-terminal (ab181216), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAND1 antibody [EPR14242(B)] - C-terminal (ab181216)

    Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue labeling CAND1 with ab181216 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-CAND1 antibody [EPR14242(B)] - C-terminal (ab181216), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-CAND1 antibody [EPR14242(B)] - C-terminal (ab181216)

    Intracellular flow cytometric analysis of Jurkat cells (2% paraformaldehyde-fixed) labeling CAND1 with ab181216 at 1/20 dilution (red)or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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