Rabbit Recombinant Monoclonal CAND1 antibody. C-terminal. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples.
IgG
Rabbit
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
IP | WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
---|---|---|---|---|---|
Human | Tested | Tested | Tested | Tested | Tested |
Mouse | Predicted | Predicted | Predicted | Predicted | Predicted |
Rat | Predicted | Predicted | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/40 - 1/60 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/10000 - 1/50000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/20 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/50 - 1/100 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
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Key assembly factor of SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complexes that promotes the exchange of the substrate-recognition F-box subunit in SCF complexes, thereby playing a key role in the cellular repertoire of SCF complexes. Acts as a F-box protein exchange factor. The exchange activity of CAND1 is coupled with cycles of neddylation conjugation: in the deneddylated state, cullin-binding CAND1 binds CUL1-RBX1, increasing dissociation of the SCF complex and promoting exchange of the F-box protein. Probably plays a similar role in other cullin-RING E3 ubiquitin ligase complexes.
KIAA0829, TIP120, TIP120A, CAND1, Cullin-associated NEDD8-dissociated protein 1, Cullin-associated and neddylation-dissociated protein 1, TBP-interacting protein of 120 kDa A, p120 CAND1, TBP-interacting protein 120A
Rabbit Recombinant Monoclonal CAND1 antibody. C-terminal. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples.
IgG
Rabbit
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR14242(B)
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This supplementary information is collated from multiple sources and compiled automatically.
CAND1 also known as cullin-associated and neddylation-dissociated 1 is a scaffolding protein with a molecular mass of approximately 130 kDa. It is found in the cytoplasm and nucleus of eukaryotic cells. CAND1 plays a critical role in regulating the activity of cullin-RING ubiquitin ligases (CRLs) which are important for protein degradation processes. By interacting with neddylated cullin proteins CAND1 modulates the assembly and disassembly of these CRL complexes therefore influencing their activity.
CAND1 mediates the dynamics of CRL complexes acting as a regulator of substrate specificity and turnover. CAND1 does not function alone; it is integral to a complex that includes cullin proteins and various CRL adaptors. This interaction ensures the right substrates are targeted for ubiquitination leading to proteasomal degradation. The ability of CAND1 to bind to cullin substrates is influenced by cullin neddylation and deneddylation cycles making it essential for the proper function of the ubiquitin-proteasome system.
Studies have shown that CAND1 is important in the ubiquitin-proteasome pathway along with the neddylation pathway. Within these pathways it primarily interacts with proteins such as cullins 1 3 and 4 which are central to CRLs. By regulating these interactions CAND1 ensures that protein turnover is controlled which is important for processes like cell cycle progression and signal transduction.
Research suggests a connection between CAND1 dysregulation and certain cancers as well as neurodegenerative disorders. In cancer aberrant regulation of CAND1 can lead to uncontrolled cell proliferation due to faulty protein degradation. In neurodegenerative diseases ineffective CAND1-mediated pathways may contribute to the accumulation of damaged proteins. Understanding CAND1's relationship with key proteins in these conditions such as p53 in cancer highlights its potential as a therapeutic target.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Immunofluorescent analysis of HeLa cells (-20°C Acetone-fixed) labeling CAND1 with ab181216 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 dilution and counterstained with Dapi (blue).
All lanes: Western blot - Anti-CAND1 antibody [EPR14242(B)] - C-terminal (ab181216) at 1/50000 dilution
Lane 1: 293T cell lysate at 20 µg
Lane 2: HeLa cell lysate at 20 µg
Lane 3: Jurkat cell lysate at 20 µg
Lane 4: Saos-2 cell lysate at 20 µg
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 136 kDa
Western blot analysis of immunoprecipitation pellet from Hela cell lysate immunoprecipitated using ab181216 at 1/50 dilution.
Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
All lanes: Immunoprecipitation - Anti-CAND1 antibody [EPR14242(B)] - C-terminal (ab181216)
Predicted band size: 136 kDa
Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue labeling CAND1 with ab181216 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Intracellular flow cytometric analysis of Jurkat cells (2% paraformaldehyde-fixed) labeling CAND1 with ab181216 at 1/20 dilution (red)or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
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