Anti-Cannabinoid Receptor II antibody (ab3561) is a rabbit polyclonal antibody that is used to detect Cannabinoid Receptor II in Western Blot, IHC-P. Suitable for Human, Rat, samples.
- Over 80 publications
- Trusted since 2003
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- Nutrients 15:2023Administration of Linoleoylethanolamide Reduced Weight Gain, Dyslipidemia, and Inflammation Associated with High-Fat-Diet-Induced Obesity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRubén Tovar,Marialuisa de Ceglia,Massimo Ubaldi,Miguel Rodríguez-Pozo,Laura Soverchia,Carlo Cifani,Gema Rojo,Ana Gavito,Laura Hernandez-Folgado,Nadine Jagerovic,Roberto Ciccocioppo,Elena Baixeras,Fernando Rodríguez de Fonseca,Juan DecaraPubMed 37892524
- Frontiers in endocrinology 14:12693342023The interplay between kisspeptin and endocannabinoid systems modulates male hypothalamic and gonadic control of reproduction .Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarianna Marino,Raffaella D'Auria,Elena Mele,Grazia Maria Giovanna Pastorino,Paola Di Pietro,Stefania D'Angelo,Natalia Della Rocca,Francesca Felicia Operto,Carmine Vecchione,Silvia Fasano,Riccardo Pierantoni,Andrea Viggiano,Rosaria Meccariello,Antonietta SantoroPubMed 37900144
- Biology 12:2023The Expression of the Endocannabinoid Receptors CB2 and GPR55 Is Highly Increased during the Progression of Alzheimer's Disease in Knock-In Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDina Medina-Vera,Hongjing Zhao,Erika Bereczki,Cristina Rosell-Valle,Makoto Shimozawa,Gefei Chen,Fernando Rodríguez de Fonseca,Per Nilsson,Simone TambaroPubMed 37372090
- Frontiers in chemistry 10:9840692022Design, synthesis and biological evaluation of novel orthosteric-allosteric ligands of the cannabinoid receptor type 2 (CBR).Applications:Unspecified applicationReactive species:Unspecified reactive speciesRebecca Ferrisi,Francesca Gado,Beatrice Polini,Caterina Ricardi,Kawthar A Mohamed,Lesley A Stevenson,Gabriella Ortore,Simona Rapposelli,Giuseppe Saccomanni,Roger G Pertwee,Robert B Laprairie,Clementina Manera,Grazia ChielliniPubMed 36238097
- Frontiers in cellular neuroscience 16:9022782022-acylethanolamide metabolizing enzymes are upregulated in human neural progenitor-derived neurons exposed to sub-lethal oxidative stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesR Scott Duncan,Sean M Riordan,Conner W Hall,Andrew J Payne,Kent D Chapman,Peter KoulenPubMed 36003139
- Cell death discovery 8:2732022Cannabinoid Receptor-1 suppresses M2 macrophage polarization in colorectal cancer by downregulating EGFR.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYou-Ming Deng,Cheng Zhao,Lei Wu,Zhan Qu,Xin-Yu WangPubMed 35641479
- Molecules (Basel, Switzerland) 27:2022Cannabidiol Improves Antioxidant Capacity and Reduces Inflammation in the Lungs of Rats with Monocrotaline-Induced Pulmonary Hypertension.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnna Krzyżewska,Marta Baranowska-Kuczko,Anna Jastrząb,Irena Kasacka,Hanna KozłowskaPubMed 35630804
- Aging and disease 13:552-5672022CB2R Attenuates Intervertebral Disc Degeneration by Delaying Nucleus Pulposus Cell Senescence through AMPK/GSK3β Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiacheng Du,Menglei Xu,Fanchen Kong,Pengfei Zhu,Yubo Mao,Yijie Liu,Hong Zhou,Zhongchen Dong,Zilin Yu,Tong Du,Ye Gu,Xiexing Wu,Dechun Geng,Haiqing MaoPubMed 35371598
- Frontiers in pharmacology 13:8617992022Electroacupuncture Reduces Visceral Pain Via Cannabinoid CB2 Receptors in a Mouse Model of Inflammatory Bowel Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHong Zhang,Wei He,Xue-Fei Hu,Yan-Zhen Li,Yong-Min Liu,Wen-Qiang Ge,Ou-Yang Zhanmu,Chao Chen,Yu-Ye Lan,Yang-Shuai Su,Xiang-Hong Jing,Bing Zhu,Hui-Lin Pan,Ling-Ling Yu,Man LiPubMed 35401205
- Cell death discovery 8:1102022New AKT-dependent mechanisms of anti-COVID-19 action of high-CBD Cannabis sativa extracts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBo Wang,Dongping Li,Anna Fiselier,Igor Kovalchuk,Olga KovalchukPubMed 35277472
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Rat Cnr2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link Q9QZN9
Reactivity data
Select an application| ICC | WB | IHC-P | |
|---|---|---|---|
| Human | Expected | Tested | Tested |
| Rat | Expected | Tested | Expected |
| Transfected cell line - Rat | Tested | Not recommended | Not recommended |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Rat | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/200 | Notes - |
Species Human | Dilution info 1/50.00000 - 1/500.00000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10.00000 - 1/100.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Heterotrimeric G protein-coupled receptor for endocannabinoid 2-arachidonoylglycerol mediating inhibition of adenylate cyclase. May function in inflammatory response, nociceptive transmission and bone homeostasis.
Alternative names
CB2A, CB2B, CNR2, Cannabinoid receptor 2, CB-2, CB2, hCB2, CX5
Recommended products
Anti-Cannabinoid Receptor II antibody (ab3561) is a rabbit polyclonal antibody that is used to detect Cannabinoid Receptor II in Western Blot, IHC-P. Suitable for Human, Rat, samples.
- Over 80 publications
- Trusted since 2003
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Rat Cnr2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link Q9QZN9
- Purification technique
- Affinity purification Immunogen
- Specificity
We have had mixed results for use of this antibody in mouse. Thus, we are removing mouse as a guaranteed application and welcome any feedback from customers who have used this antibody in mouse.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-Cannabinoid Receptor II antibody (ab3561) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), in Human, Rat, samples.
What is the molecular weight of Cannabinoid Receptor II?
Anti-Cannabinoid Receptor II (ab3561) specifically detects a band for Cannabinoid Receptor II (UniProt: Q9QZN9) at a molecular weight of 40kDa.
Recommended positive controls
WB: HT29, C6, rat colon cell lysate;IHC: human tonsil tissue, skin tissue;ICC: AtT20 cells transfected with rat CB2 gene
Trusted by the scientific community
Anti-Cannabinoid Receptor II (ab3561) was first used in a scientific publication in 2003 and has been cited over 80 times in peer-reviewed journals.
Reviewed by scientists
Anti-Cannabinoid Receptor II (ab3561) has over 15 independent reviews from customers.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cannabinoid Receptor II antibody (ab3561)
ab3561 labelling Cannabinoid Receptor II in the cytoplasm of Human tonsil tissue (right) compared with a negative control (left) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:20 in 3% BSA-PBS) overnight at 4°C. A anti-rabbit HRP Goat Anti-Rabbit IgG H&L (HRP) ab6721 was used as the secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cannabinoid Receptor II antibody (ab3561)
ab3561 labelling Cannabinoid Receptor II in the cytoplasm of Human skin tissue (right) compared with a negative control (left) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tssiue sections were incubated with the primary antibody (1:20 in 3% BSA-PBS) overnight at 4°C. A anti-rabbit HRP (Goat Anti-Rabbit IgG H&L (HRP) ab6721) was used as the secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
Western blot - Anti-Cannabinoid Receptor II antibody (ab3561)
All lanes: Western blot - Anti-Cannabinoid Receptor II antibody (ab3561) at 1/200 dilution
Lane 1: HT29 cell lysate at 25 µg
Lane 2: C6 cell lysate at 25 µg
Lane 3: Rat colon cell lysate at 25 µg
Predicted band size: 40 kDa
Observed band size: 40 kDa
Immunocytochemistry - Anti-Cannabinoid Receptor II antibody (ab3561)
Immunocytochemistry/immunofluorescence analysis of AtT20 cells transfect with the rat CB2 gene labeling Cannabinoid Receptor II with ab3561.
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