Anti-Cannabinoid Receptor II antibody

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cannabinoid Receptor II antibody (AB3561)

ab3561 labelling Cannabinoid Receptor II in the cytoplasm of Human tonsil tissue (right) compared with a negative control (left) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1 : 20 in 3% BSA-PBS) overnight at 4°C. A anti-rabbit HRP ab6721 was used as the secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cannabinoid Receptor II antibody (AB3561)

ab3561 labelling Cannabinoid Receptor II in the cytoplasm of Human skin tissue (right) compared with a negative control (left) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tssiue sections were incubated with the primary antibody (1 : 20 in 3% BSA-PBS) overnight at 4°C. A anti-rabbit HRP (ab6721) was used as the secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

• ICC

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Immunocytochemistry - Anti-Cannabinoid Receptor II antibody (AB3561)

Immunocytochemistry/immunofluorescence analysis of AtT20 cells transfect with the rat CB2 gene labeling Cannabinoid Receptor II with ab3561.

• WB

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Western blot - Anti-Cannabinoid Receptor II antibody (AB3561)

All lanes:

Western blot - Anti-Cannabinoid Receptor II antibody (ab3561) at 1/200 dilution

Lane 1:

HT29 cell lysate at 25 µg

Lane 2:

C6 cell lysate at 25 µg

Lane 3:

Rat colon cell lysate at 25 µg

Predicted band size: 40 kDa

Observed band size: 40 kDa

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