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Rabbit Recombinant Monoclonal CANT1 antibody. Suitable for WB and reacts with Human samples.

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Images

Western blot - Anti-CANT1 antibody [EPR29983-518] (AB323618), expandable thumbnail
  • Western blot - Anti-CANT1 antibody [EPR29983-518] (AB323618), expandable thumbnail
  • Western blot - Anti-CANT1 antibody [EPR29983-518] (AB323618), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Not recommended
Mouse
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse
Dilution info
-
Notes

-

Target data

Function

Calcium-dependent nucleotidase with a preference for UDP. The order of activity with different substrates is UDP > GDP > UTP > GTP. Has very low activity towards ADP and even lower activity towards ATP. Does not hydrolyze AMP and GMP (PubMed:12234496, PubMed:15006348, PubMed:15248776, PubMed:16835225). Involved in proteoglycan synthesis (PubMed:22539336).

Alternative names

SHAPY, CANT1, Soluble calcium-activated nucleotidase 1, SCAN-1, Apyrase homolog, Putative MAPK-activating protein PM09, Putative NF-kappa-B-activating protein 107

Rabbit Recombinant Monoclonal CANT1 antibody. Suitable for WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR29983-518
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-CANT1 antibody [EPR29983-518] (ab323618), expandable thumbnail

    Western blot - Anti-CANT1 antibody [EPR29983-518] (ab323618)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-CANT1 antibody [EPR29983-518] (ab323618) at 1/1000 dilution

    Lane 1: Huh7 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 40 µg

    Lane 2: LNCaP (human prostate carcinoma epithelial cell) whole cell lysate at 40 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 40-45 kDa, 36 kDa

    Exposure time: 180s

  • Western blot - Anti-CANT1 antibody [EPR29983-518] (ab323618), expandable thumbnail

    Western blot - Anti-CANT1 antibody [EPR29983-518] (ab323618)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: cerebellum, liver (PMID: 12234496)

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 22451650).

    Exposure time: Lane 1: 70 seconds, lane 2-3: 180 seconds

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-CANT1 antibody [EPR29983-518] (ab323618) at 1/1000 dilution

    Lane 1: Human colon tissue lysate at 40 µg

    Lane 2: Human testis tissue lysate at 40 µg

    Lane 3: Human small intestine tissue lysate at 40 µg

    Lane 4: Human cerebellum tissue lysate at 40 µg

    Lane 5: Human liver tissue lysate at 40 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 40-45 kDa, 36 kDa

  • Western blot - Anti-CANT1 antibody [EPR29983-518] (ab323618), expandable thumbnail

    Western blot - Anti-CANT1 antibody [EPR29983-518] (ab323618)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 22451650).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-CANT1 antibody [EPR29983-518] (ab323618) at 1/1000 dilution

    Lane 1: A431 (human epidermoid carcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

    Lane 2: A431 transfected with siRNA specifically targeting CANT1 whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 40-45 kDa, 36 kDa

    Exposure time: 145s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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