AB88446

Anti-CAP1 antibody

Name: Anti-CAP1 antibody (ab88446) | Abcam
Brand: Abcam Limited
SKU: AB88446
Price: 535 USD
Availability: InStock

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(2 Publications)

Mouse Polyclonal CAP1 antibody. Carrier free. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CAP1.

View Alternative Names

CAP, CAP1, Adenylyl cyclase-associated protein 1, CAP 1

6 Images

Flow Cytometry - Anti-CAP1 antibody (AB88446)

Flow Cyt

Unknown

Flow Cytometry - Anti-CAP1 antibody (AB88446)

FACS analysis of (a) negative control unstained, (b) negative control goat anti-mouse-FITC stained and (c) ab88446 stained methanol-treated human promonocytic THP-1 cells.

Flow Cyt

Unknown

Flow Cytometry - Anti-CAP1 antibody (AB88446)

FACS analysis of (a) negative control unstained, (b) negative control goat anti-mouse-FITC stained and (c) ab88446 stained methanol-treated human HeLa cells.

Immunocytochemistry/ Immunofluorescence - Anti-CAP1 antibody (AB88446)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CAP1 antibody (AB88446)

Immunofluorescence image staining human CAP1 in HeLa cells, using ab88446 at 10μg/ml.

Unknown

Western blot - Anti-CAP1 antibody (AB88446)

All lanes:

Western blot - Anti-CAP1 antibody (ab88446) at 1 µg/mL

All lanes:

A-431 cell lysate at 50 µg

Secondary

All lanes:

Goat Anti-Mouse IgG at 1/2500 dilution

Predicted band size: 52 kDa

Observed band size: 52 kDa

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Western blot - Anti-CAP1 antibody (AB88446)

All lanes:

Western blot - Anti-CAP1 antibody (ab88446) at 1 µg/mL

All lanes:

Human colon tissue at 50 µg

Secondary

All lanes:

Goat Anti-Mouse IgG at 1/2500 dilution

Predicted band size: 52 kDa

Observed band size: 52 kDa

false

Unknown

Western blot - Anti-CAP1 antibody (AB88446)

All lanes:

Western blot - Anti-CAP1 antibody (ab88446) at 1 µg/mL

Lane 1:

Transfected 293T cell line at 50 µg

Lane 2:

Non-transfected cell line at 50 µg

Secondary

All lanes:

Goat Anti-Mouse IgG at 1/2500 dilution

Predicted band size: 52 kDa

Observed band size: 52 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, Flow Cyt, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CAP1.

Q01518

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg-isotype-control-ab37355'>ab37355</a> - Mouse polyclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The CAP1 protein also known as Cyclase-associated protein 1 is a dynamic actin-binding protein found in various tissues throughout the body. CAP1 has a mass of approximately 53 kDa. It is predominantly expressed in immune cells and muscle tissue. Mechanically CAP1 interacts with actin filaments promoting their disassembly and turnover therefore facilitating cytoskeletal restructuring critical for cell motility and shape changes.

Biological function summary

CAP1 plays a significant role in cellular signaling and organization. It does not exist as an isolated entity but instead forms complexes with actin and other cytoskeletal proteins to exert its functions. By promoting actin turnover CAP1 influences processes like intracellular trafficking and cell migration. This protein also contributes to the regulation of signal transduction pathways connected to cell proliferation and differentiation.

Pathways

CAP1 is involved in the reorganization of the actin cytoskeleton and the Wnt signaling pathway. In actin reorganization CAP1 works closely with cofilin another actin-binding protein to enhance actin dynamics. Through the Wnt pathway CAP1 modulates transcriptional activity and cellular responses to growth signals. These interactions highlight CAP1's integration into important pathways that regulate cell behavior.

CAP1 is relevant in cancer progression and rheumatoid arthritis. In cancer abnormal regulation of CAP1 can lead to enhanced cell migration and invasion which are hallmarks of metastasis. Its interaction with proteins such as cofilin in cytoskeletal rearrangement impacts tumor cell dynamics. In rheumatoid arthritis altered CAP1 activity affects immune cell function and joint degradation. These connections highlight CAP1's importance in pathological conditions where actin dynamics and immune responses play critical roles.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Directly regulates filament dynamics and has been implicated in a number of complex developmental and morphological processes, including mRNA localization and the establishment of cell polarity.

See full target information CAP1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 34:11292-11306 PubMed32602619

2020

AIP1 and Cofilin control the actin dynamics to modulate the asymmetric division and cytokinesis in mouse oocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Zhe-Long Jin,Xue-Rui Yao,Liu Wen,Guo Hao,Jeong-Woo Kwon,Jiang Hao,Nam-Hyung Kim

Journal of cell science 131: PubMed30404832

2018

CAP1-mediated actin cycling via ADF/cofilin proteins is essential for asymmetric division in mouse oocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Zhe-Long Jin,Yu-Jin Jo,Suk Namgoong,Nam-Hyung Kim

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CAP1 antibody

Flow Cytometry - Anti-CAP1 antibody (AB88446)

Flow Cytometry - Anti-CAP1 antibody (AB88446)

Immunocytochemistry/ Immunofluorescence - Anti-CAP1 antibody (AB88446)

Western blot - Anti-CAP1 antibody (AB88446)

Western blot - Anti-CAP1 antibody (AB88446)

Western blot - Anti-CAP1 antibody (AB88446)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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