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AB96354

Anti-CAP1 antibody

4

(1 Review)

|

(2 Publications)

Rabbit Polyclonal CAP1 antibody. Suitable for WB, IHC-P and reacts with Human, Rat samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CAP1 aa 250 to C-terminus.

View Alternative Names

CAP, CAP1, Adenylyl cyclase-associated protein 1, CAP 1

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAP1 antibody (AB96354)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAP1 antibody (AB96354)

ab96354 at 1/500 dilution staining paraffin-embedded DLD1 xenograft

Western blot - Anti-CAP1 antibody (AB96354)
  • WB

Supplier Data

Western blot - Anti-CAP1 antibody (AB96354)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-CAP1 antibody (ab96354) at 1/3000 dilution

Lane 1:

Neuro2a whole cell extracts at 30 µg

Lane 2:

C8D30 whole cell extracts at 30 µg

Lane 3:

NIH-3T3 whole cell extracts at 30 µg

Lane 4:

Raw264.7 whole cell extracts at 30 µg

Lane 5:

C2C12 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 52 kDa

false

Western blot - Anti-CAP1 antibody (AB96354)
  • WB

Supplier Data

Western blot - Anti-CAP1 antibody (AB96354)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-CAP1 antibody (ab96354) at 1/3000 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

A431 whole cell extracts at 30 µg

Lane 3:

HeLa whole cell extracts at 30 µg

Lane 4:

HepG2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 52 kDa

false

Western blot - Anti-CAP1 antibody (AB96354)
  • WB

Supplier Data

Western blot - Anti-CAP1 antibody (AB96354)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-CAP1 antibody (ab96354) at 1/3000 dilution

Lane 1:

PC12 whole cell extracts at 30 µg

Lane 2:

Rat2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 52 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human CAP1 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q01518

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The CAP1 protein also known as Cyclase-associated protein 1 is a dynamic actin-binding protein found in various tissues throughout the body. CAP1 has a mass of approximately 53 kDa. It is predominantly expressed in immune cells and muscle tissue. Mechanically CAP1 interacts with actin filaments promoting their disassembly and turnover therefore facilitating cytoskeletal restructuring critical for cell motility and shape changes.
Biological function summary

CAP1 plays a significant role in cellular signaling and organization. It does not exist as an isolated entity but instead forms complexes with actin and other cytoskeletal proteins to exert its functions. By promoting actin turnover CAP1 influences processes like intracellular trafficking and cell migration. This protein also contributes to the regulation of signal transduction pathways connected to cell proliferation and differentiation.

Pathways

CAP1 is involved in the reorganization of the actin cytoskeleton and the Wnt signaling pathway. In actin reorganization CAP1 works closely with cofilin another actin-binding protein to enhance actin dynamics. Through the Wnt pathway CAP1 modulates transcriptional activity and cellular responses to growth signals. These interactions highlight CAP1's integration into important pathways that regulate cell behavior.

CAP1 is relevant in cancer progression and rheumatoid arthritis. In cancer abnormal regulation of CAP1 can lead to enhanced cell migration and invasion which are hallmarks of metastasis. Its interaction with proteins such as cofilin in cytoskeletal rearrangement impacts tumor cell dynamics. In rheumatoid arthritis altered CAP1 activity affects immune cell function and joint degradation. These connections highlight CAP1's importance in pathological conditions where actin dynamics and immune responses play critical roles.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Directly regulates filament dynamics and has been implicated in a number of complex developmental and morphological processes, including mRNA localization and the establishment of cell polarity.
See full target information CAP1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature cell biology 21:592-602 PubMed30962575

2019

A complex containing lysine-acetylated actin inhibits the formin INF2.

Applications

Unspecified application

Species

Unspecified reactive species

Mu A,Tak Shun Fung,Arminja N Kettenbach,Rajarshi Chakrabarti,Henry N Higgs

Gene 692:185-194 PubMed30682386

2019

Various exercise intensities differentially regulate GAP-43 and CAP-1 expression in the rat hippocampus.

Applications

Unspecified application

Species

Unspecified reactive species

Masoud Rahmati,Abdolreza Kazemi
View all publications
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Product promise

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