AB249174

Anti-CAP1 antibody [EPR8339(B)] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal CAP1 antibody. Carrier free. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

CAP, CAP1, Adenylyl cyclase-associated protein 1, CAP 1

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAP1 antibody [EPR8339(B)] - BSA and Azide free (AB249174)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAP1 antibody [EPR8339(B)] - BSA and Azide free (AB249174)

This data was developed using ab155079, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue using ab155079 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAP1 antibody [EPR8339(B)] - BSA and Azide free (AB249174)

This data was developed using ab155079, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human normal liver tissue using ab155079 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAP1 antibody [EPR8339(B)] - BSA and Azide free (AB249174)

This data was developed using ab155079, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human lung adenocarcinoma tissue using ab155079 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAP1 antibody [EPR8339(B)] - BSA and Azide free (AB249174)

This data was developed using ab155079, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human gastric adenocarcinoma tissue using ab155079 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-CAP1 antibody [EPR8339(B)] - BSA and Azide free (AB249174)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-CAP1 antibody [EPR8339(B)] - BSA and Azide free (AB249174)

This data was developed using ab155079, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized 293 cells, labeling CAP1 with ab155079 at 1/10 dilution (red) or Rabiit IgG negative control (green).

Unknown

Western blot - Anti-CAP1 antibody [EPR8339(B)] - BSA and Azide free (AB249174)

This data was developed using ab155079, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-CAP1 antibody [EPR8339(B)] (<a href='/en-us/products/primary-antibodies/cap1-antibody-epr8339b-ab155079'>ab155079</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

A431 cell lysate at 10 µg

Lane 4:

HepG2 cell lysate at 10 µg

Lane 5:

Caco-2 cell lysate at 10 µg

Predicted band size: 52 kDa

false

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAP1 antibody [EPR8339(B)] - BSA and Azide free (AB249174)

This data was developed using ab155079, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded prostate tissue, labeling CAP1 with ab155079 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unconjugated

Anti-CAP1 antibody [EPR8339(B)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8339(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

ab249174 is the carrier-free version of ab155079.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The CAP1 protein also known as Cyclase-associated protein 1 is a dynamic actin-binding protein found in various tissues throughout the body. CAP1 has a mass of approximately 53 kDa. It is predominantly expressed in immune cells and muscle tissue. Mechanically CAP1 interacts with actin filaments promoting their disassembly and turnover therefore facilitating cytoskeletal restructuring critical for cell motility and shape changes.

Biological function summary

CAP1 plays a significant role in cellular signaling and organization. It does not exist as an isolated entity but instead forms complexes with actin and other cytoskeletal proteins to exert its functions. By promoting actin turnover CAP1 influences processes like intracellular trafficking and cell migration. This protein also contributes to the regulation of signal transduction pathways connected to cell proliferation and differentiation.

Pathways

CAP1 is involved in the reorganization of the actin cytoskeleton and the Wnt signaling pathway. In actin reorganization CAP1 works closely with cofilin another actin-binding protein to enhance actin dynamics. Through the Wnt pathway CAP1 modulates transcriptional activity and cellular responses to growth signals. These interactions highlight CAP1's integration into important pathways that regulate cell behavior.

CAP1 is relevant in cancer progression and rheumatoid arthritis. In cancer abnormal regulation of CAP1 can lead to enhanced cell migration and invasion which are hallmarks of metastasis. Its interaction with proteins such as cofilin in cytoskeletal rearrangement impacts tumor cell dynamics. In rheumatoid arthritis altered CAP1 activity affects immune cell function and joint degradation. These connections highlight CAP1's importance in pathological conditions where actin dynamics and immune responses play critical roles.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Directly regulates filament dynamics and has been implicated in a number of complex developmental and morphological processes, including mRNA localization and the establishment of cell polarity.

See full target information CAP1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CAP1 antibody [EPR8339(B)] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAP1 antibody [EPR8339(B)] - BSA and Azide free (AB249174)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAP1 antibody [EPR8339(B)] - BSA and Azide free (AB249174)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAP1 antibody [EPR8339(B)] - BSA and Azide free (AB249174)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAP1 antibody [EPR8339(B)] - BSA and Azide free (AB249174)

Flow Cytometry (Intracellular) - Anti-CAP1 antibody [EPR8339(B)] - BSA and Azide free (AB249174)

Western blot - Anti-CAP1 antibody [EPR8339(B)] - BSA and Azide free (AB249174)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAP1 antibody [EPR8339(B)] - BSA and Azide free (AB249174)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise