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AB133558

Anti-CAP2 antibody [EPR6378]

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(1 Publication)

Rabbit Recombinant Monoclonal CAP2 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Adenylyl cyclase-associated protein 2, CAP 2, CAP2

3 Images
Flow Cytometry (Intracellular) - Anti-CAP2 antibody [EPR6378] (AB133558)
  • Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-CAP2 antibody [EPR6378] (AB133558)

Intracellular flow cytometric analysis of permeabilized Saos 2 cells labelling CAP2 with ab133558 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

Western blot - Anti-CAP2 antibody [EPR6378] (AB133558)
  • WB

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Western blot - Anti-CAP2 antibody [EPR6378] (AB133558)

All lanes:

Western blot - Anti-CAP2 antibody [EPR6378] (ab133558) at 1/1000 dilution

Lane 1:

Saos 2 cell lysate at 10 µg

Lane 2:

A673 cell lysate at 10 µg

Lane 3:

Human testis cell lysate at 10 µg

Lane 4:

Human heart cell lysate at 10 µg

Lane 5:

Human fetal muscle cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 53 kDa

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OI-RD Scanning - Anti-CAP2 antibody [EPR6378] (AB133558)
  • OI-RD Scanning

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OI-RD Scanning - Anti-CAP2 antibody [EPR6378] (AB133558)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6378

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CAP2 also known as Cyclase Associated Protein 2 is a protein playing an essential role in actin dynamics. It has a molecular mass of approximately 54 kDa. Researchers have detected its expression in various tissues including the heart skeletal muscle and brain. CAP2 influences the organization of the cytoskeleton by binding to actin filaments and regulating their polymerization and depolymerization. Its activity makes it an important player in cell motility and structure maintenance.
Biological function summary

CAP2 contributes to cellular processes by modulating actin filament turnover and facilitating actin recycling. It operates as part of a protein complex that includes other actin-binding proteins often working in tandem with profilin and cofilin. This complex formation is integral to the regulation of cytoskeletal organization impacting important cellular functions like cell division intracellular transport and signal transduction.

Pathways

CAP2 is an active participant in pathways controlling cell movement and structural integrity particularly the actin cytoskeleton signaling pathway. In this role CAP2 interacts with proteins such as cofilin which also modulates actin dynamics to maintain a balance of actin filament assembly and disassembly. Another pathway CAP2 is involved in is the cell adhesion pathway where it helps facilitate the dynamic remodeling necessary for cell attachment and migration.

CAP2 has been linked to cardiac diseases and cancer. Alterations in CAP2 expression have been associated with the development of dilated cardiomyopathy a condition marked by weakened heart muscle functionality. Furthermore CAP2 has demonstrated interactions with p53 a protein often mutated in cancers implicating it in processes that may contribute to tumor growth and metastasis. These connections reveal its importance not only in normal cellular function but also in pathological states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in the regulation of actin polymerization.
See full target information Adenylyl cyclase-associated protein 2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of cell biology 224: PubMed40304687

2025

Extracellular vesicles adhere to cells primarily by interactions of integrins and GM1 with laminin.

Applications

Unspecified application

Species

Unspecified reactive species

Tatsuki Isogai,Koichiro M Hirosawa,Miki Kanno,Ayano Sho,Rinshi S Kasai,Naoko Komura,Hiromune Ando,Keiko Furukawa,Yuhsuke Ohmi,Koichi Furukawa,Yasunari Yokota,Kenichi G N Suzuki
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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