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AB190686

Anti-CAPON/NOS1AP antibody [EPR14519(B)]

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(5 Publications)

Rabbit Recombinant Monoclonal CAPON/NOS1AP antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 5 publications.

View Alternative Names

CAPON, KIAA0464, NOS1AP, Carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein, C-terminal PDZ ligand of neuronal nitric oxide synthase protein, Nitric oxide synthase 1 adaptor protein

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-CAPON/NOS1AP antibody [EPR14519(B)] (AB190686)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CAPON/NOS1AP antibody [EPR14519(B)] (AB190686)

Immunofluorescence analysis of 4% paraformaldehyde-fixed SH-SY5Y cells labeling CAPON/NOS1AP with ab190686 at 1/50 dilution. Goat anti-rabbit IgG (Alexa Fluor® 555) at 1/200 dilution was used as the secondary antibody (red). Slide is counter stained with Dapi (blue).

Flow Cytometry (Intracellular) - Anti-CAPON/NOS1AP antibody [EPR14519(B)] (AB190686)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-CAPON/NOS1AP antibody [EPR14519(B)] (AB190686)

Intracellular flow cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling CAPON/NOS1AP with ab190686 at 1/130 dilution (red) compared with a rabbit monoclonal IgG control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

Western blot - Anti-CAPON/NOS1AP antibody [EPR14519(B)] (AB190686)
  • WB

Supplier Data

Western blot - Anti-CAPON/NOS1AP antibody [EPR14519(B)] (AB190686)

All lanes:

Western blot - Anti-CAPON/NOS1AP antibody [EPR14519(B)] (ab190686) at 1/10000 dilution

All lanes:

Human fetal brain lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 56 kDa

false

Western blot - Anti-CAPON/NOS1AP antibody [EPR14519(B)] (AB190686)
  • WB

Supplier Data

Western blot - Anti-CAPON/NOS1AP antibody [EPR14519(B)] (AB190686)

All lanes:

Western blot - Anti-CAPON/NOS1AP antibody [EPR14519(B)] (ab190686) at 1/1000 dilution

Lane 1:

Human fetal brain lysates at 20 µg

Lane 2:

Human cerebellum brain lysates at 20 µg

Lane 3:

MCF-7 cell lysates at 20 µg

Lane 4:

HeLa cell lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 56 kDa

false

Western blot - Anti-CAPON/NOS1AP antibody [EPR14519(B)] (AB190686)
  • WB

Supplier Data

Western blot - Anti-CAPON/NOS1AP antibody [EPR14519(B)] (AB190686)

All lanes:

Western blot - Anti-CAPON/NOS1AP antibody [EPR14519(B)] (ab190686) at 1/1000 dilution

Lane 1:

Mouse brain lysates at 10 µg

Lane 2:

Rat brain lysates at 10 µg

Lane 3:

C6 cell lysates at 10 µg

Lane 4:

Raw 264.7 cell lysates at 10 µg

Lane 5:

PC-12 cell lysates at 10 µg

Lane 6:

NIH/3T3 cell lysates at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 56 kDa

false

  • Carrier free

    Anti-CAPON/NOS1AP antibody [EPR14519(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14519(B)

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/130", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CAPON also known as NOS1AP (nitric oxide synthase 1 adaptor protein) is an adaptor protein with a molecular weight of about 55 kDa. This protein interacts primarily with neuronal nitric oxide synthase (nNOS) modulating its activity. CAPON is expressed in the brain particularly in regions like the hippocampus and cerebral cortex where it plays roles in neuronal signaling. Its involvement in the brain implies its importance in neural functions and cognitive processes.
Biological function summary

CAPON influences signal transduction processes involving nitric oxide. As part of a molecular complex it binds with nNOS which leads to the modulation of nitric oxide production. This regulation impacts synaptic plasticity and neurotransmission hence affecting learning and memory. By interacting with other signaling proteins CAPON can affect the cellular response to various stimuli making it a significant player in neuronal communication.

Pathways

CAPON’s function fits into the nitric oxide signaling pathway which is important for neuron communication and vasodilation processes. It associates with the ERK/MAPK pathway which plays a role in cognition and neural development. Through nitric oxide modulation it influences other proteins such as PSD95 a postsynaptic density protein which impacts synaptic organization and function. This involvement showcases its role in maintaining neuronal integrity and function within these pathways.

CAPON has ties to neurologic and psychiatric conditions specifically schizophrenia and bipolar disorder. Alterations in its interaction with nNOS have been linked to the pathophysiology of these conditions. Additionally CAPON's connection with proteins like PSD95 suggests it may impact synaptic organization which can be disrupted in such mental health disorders. Understanding CAPON’s role could contribute to therapeutic approaches in these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Adapter protein involved in neuronal nitric-oxide (NO) synthesis regulation via its association with nNOS/NOS1. The complex formed with NOS1 and synapsins is necessary for specific NO and synapsin functions at a presynaptic level. Mediates an indirect interaction between NOS1 and RASD1 leading to enhance the ability of NOS1 to activate RASD1. Competes with DLG4 for interaction with NOS1, possibly affecting NOS1 activity by regulating the interaction between NOS1 and DLG4 (By similarity). In kidney podocytes, plays a role in podosomes and filopodia formation through CDC42 activation (PubMed : 33523862).
See full target information NOS1AP
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eado9751 PubMed39919185

2025

Inflammatory responses to acute carbon monoxide poisoning and the role of plasma gelsolin.

Applications

Unspecified application

Species

Unspecified reactive species

Awadhesh K Arya,Kinjal Sethuraman,Jaylyn Waddell,Yong Sung Cha,Yuanyuan Liang,Veena M Bhopale,Abid R Bhat,Zuha Imtiyaz,Alik Dakessian,Yoonsuk Lee,Stephen R Thom

ImmunoHorizons 7:71-80 PubMed36645851

2023

Blood-Borne Microparticles Are an Inflammatory Stimulus in Type 2 Diabetes Mellitus.

Applications

Unspecified application

Species

Unspecified reactive species

Stephen R Thom,Veena M Bhopale,Awadhesh K Arya,Deepa Ruhela,Abid R Bhat,Nandita Mitra,Ole Hoffstad,D Scot Malay,Ziad K Mirza,John C Lantis,Hadar A Lev-Tov,Robert S Kirsner,Ru-Ching Hsia,Susan L Levinson,Mark J DiNubile,David J Margolis

Theranostics 12:3656-3675 PubMed35664081

2022

Requirement of hippocampal DG nNOS-CAPON dissociation for the anxiolytic and antidepressant effects of fluoxetine.

Applications

Unspecified application

Species

Unspecified reactive species

Hu-Jiang Shi,Dan-Lian Wu,Rong Chen,Na Li,Li-Juan Zhu

British journal of pharmacology 177:3674-3690 PubMed32343840

2020

nNOS-CAPON blockers produce anxiolytic effects by promoting synaptogenesis in chronic stress-induced animal models of anxiety.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Juan Zhu,Hu-Jiang Shi,Lei Chang,Cheng Cheng Zhang,Meng Si,Na Li,Dong-Ya Zhu

International journal of clinical and experimental pathology 8:12748-56 PubMed26722464

2015

Phosphorylated neuronal nitric oxide synthase in neuropathic pain in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Zhidong Zhou,Yingping Liang,Fumou Deng,Yong Cheng,Jing Sun,Lian Guo,Guohai Xu
View all publications
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