JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB236134

Anti-CAPON/NOS1AP antibody [EPR14519(B)] - BSA and Azide free

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Recombinant Monoclonal CAPON/NOS1AP antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 2 publications.

View Alternative Names

CAPON, KIAA0464, NOS1AP, Carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein, C-terminal PDZ ligand of neuronal nitric oxide synthase protein, Nitric oxide synthase 1 adaptor protein

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-CAPON/NOS1AP antibody [EPR14519(B)] - BSA and Azide free (AB236134)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CAPON/NOS1AP antibody [EPR14519(B)] - BSA and Azide free (AB236134)

Immunofluorescence analysis of 4% paraformaldehyde-fixed SH-SY5Y cells labeling CAPON/NOS1AP with ab190686 at 1/50 dilution. Goat anti-rabbit IgG (Alexa Fluor® 555) at 1/200 dilution was used as the secondary antibody (red). Slide is counter stained with Dapi (blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab190686).

Flow Cytometry (Intracellular) - Anti-CAPON/NOS1AP antibody [EPR14519(B)] - BSA and Azide free (AB236134)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-CAPON/NOS1AP antibody [EPR14519(B)] - BSA and Azide free (AB236134)

Intracellular flow cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling CAPON/NOS1AP with ab190686 at 1/130 dilution (red) compared with a rabbit monoclonal IgG control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab190686).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14519(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB171938

Anti-SRGAP1 antibody [EPR8760(2)]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-SRGAP1 antibody [EPR8760(2)] (AB171938)

  • 0
  • 0
Primary Antibodies

AB7800

Anti-Cytokeratin 14 antibody [LL002]

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 14 antibody [LL002] (AB7800)

  • 5
  • 27
Primary Antibodies

AB134130

Anti-CLSTN1 antibody [EPR2963]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-CLSTN1 antibody [EPR2963] (AB134130)

  • 5
  • 2
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
style
left-column

Product details

ab236134 is the carrier-free version of ab190686.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CAPON also known as NOS1AP (nitric oxide synthase 1 adaptor protein) is an adaptor protein with a molecular weight of about 55 kDa. This protein interacts primarily with neuronal nitric oxide synthase (nNOS) modulating its activity. CAPON is expressed in the brain particularly in regions like the hippocampus and cerebral cortex where it plays roles in neuronal signaling. Its involvement in the brain implies its importance in neural functions and cognitive processes.
Biological function summary

CAPON influences signal transduction processes involving nitric oxide. As part of a molecular complex it binds with nNOS which leads to the modulation of nitric oxide production. This regulation impacts synaptic plasticity and neurotransmission hence affecting learning and memory. By interacting with other signaling proteins CAPON can affect the cellular response to various stimuli making it a significant player in neuronal communication.

Pathways

CAPON’s function fits into the nitric oxide signaling pathway which is important for neuron communication and vasodilation processes. It associates with the ERK/MAPK pathway which plays a role in cognition and neural development. Through nitric oxide modulation it influences other proteins such as PSD95 a postsynaptic density protein which impacts synaptic organization and function. This involvement showcases its role in maintaining neuronal integrity and function within these pathways.

CAPON has ties to neurologic and psychiatric conditions specifically schizophrenia and bipolar disorder. Alterations in its interaction with nNOS have been linked to the pathophysiology of these conditions. Additionally CAPON's connection with proteins like PSD95 suggests it may impact synaptic organization which can be disrupted in such mental health disorders. Understanding CAPON’s role could contribute to therapeutic approaches in these diseases.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Adapter protein involved in neuronal nitric-oxide (NO) synthesis regulation via its association with nNOS/NOS1. The complex formed with NOS1 and synapsins is necessary for specific NO and synapsin functions at a presynaptic level. Mediates an indirect interaction between NOS1 and RASD1 leading to enhance the ability of NOS1 to activate RASD1. Competes with DLG4 for interaction with NOS1, possibly affecting NOS1 activity by regulating the interaction between NOS1 and DLG4 (By similarity). In kidney podocytes, plays a role in podosomes and filopodia formation through CDC42 activation (PubMed : 33523862).
See full target information NOS1AP
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Open life sciences 20:20221016 PubMed40059873

2025

SIK1 inhibits IL-1β-stimulated cartilage apoptosis and inflammation through the CRTC2/CREB1 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Mangmang Chen,Luyou Ye,Shenglei Lin

Inflammatory bowel diseases 30:2259-2270 PubMed38944815

2024

Solute Transporter OCTN1/Slc22a4 Affects Disease Severity and Response to Infliximab in Experimental Colitis: Role of Gut Microbiota and Immune Modulation.

Applications

Unspecified application

Species

Unspecified reactive species

Federica Del Chierico,Letizia Masi,Valentina Petito,Valerio Baldelli,Pierluigi Puca,Roberta Benvenuto,Marco Fidaleo,Ivana Palucci,Loris Riccardo Lopetuso,Maria Emiliana Caristo,Cinzia Carrozza,Maria Cristina Giustiniani,Noritaka Nakamichi,Yukio Kato,Lorenza Putignani,Antonio Gasbarrini,Giovambattista Pani,Franco Scaldaferri
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com