AB205377

Anti-Caprin-1 antibody

Name: Anti-Caprin-1 antibody (ab205377) | Abcam
Brand: Abcam Limited
SKU: AB205377
Price: 535 USD
Availability: InStock

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(3 Publications)

Goat Polyclonal Caprin-1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human CAPRIN1 aa 450-500.

View Alternative Names

GPIAP1, GPIP137, M11S1, RNG105, CAPRIN1, Caprin-1, Cell cycle-associated protein 1, Cytoplasmic activation- and proliferation-associated protein 1, GPI-anchored membrane protein 1, GPI-anchored protein p137, Membrane component chromosome 11 surface marker 1, RNA granule protein 105, GPI-p137, p137GPI

1 Images

Supplier Data

Western blot - Anti-Caprin-1 antibody (AB205377)

The ~140 kDa band corresponds to the reported p137 protein (Ellis and Luzio, J Biol Chem. 1995 Sep 1;270(35) : 20717-23.PMID : 7657653).

All lanes:

Western blot - Anti-Caprin-1 antibody (ab205377) at 0.3 µg/mL

Lane 1:

Jurkat cell lysate (in RIPA buffer) at 35 µg

Lane 2:

HeLa cell lysate (in RIPA buffer) at 35 µg

Predicted band size: 78 kDa

Observed band size: 140 kDa,80 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human CAPRIN1 aa 450-500. The exact immunogen used to generate this antibody is proprietary information.

Q14444

Specificity

ab205377 is expected to recognize both reported isoforms (NP_005889.3; NP_976240.1).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-1 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab205377 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

mRNA-binding protein that acts as a regulator of mRNAs transport, translation and/or stability, and which is involved in neurogenesis, synaptic plasticity in neurons and cell proliferation and migration in multiple cell types (PubMed : 17210633, PubMed : 31439799, PubMed : 35979925). Plays an essential role in cytoplasmic stress granule formation (PubMed : 35977029). Acts as an mRNA regulator by mediating formation of some phase-separated membraneless compartment : undergoes liquid-liquid phase separation upon binding to target mRNAs, leading to assemble mRNAs into cytoplasmic ribonucleoprotein granules that concentrate mRNAs with associated regulatory factors (PubMed : 31439799, PubMed : 32302570, PubMed : 32302571, PubMed : 32302572, PubMed : 34074792, PubMed : 36040869, PubMed : 36279435). Undergoes liquid-liquid phase separation following phosphorylation and interaction with FMR1, promoting formation of cytoplasmic ribonucleoprotein granules that concentrate mRNAs with factors that inhibit translation and mediate deadenylation of target mRNAs (PubMed : 31439799). In these cytoplasmic ribonucleoprotein granules, CAPRIN1 mediates recruitment of CNOT7 deadenylase, leading to mRNA deadenylation and degradation (PubMed : 31439799). Binds directly and selectively to MYC and CCND2 mRNAs (PubMed : 17210633). In neuronal cells, directly binds to several mRNAs associated with RNA granules, including BDNF, CAMK2A, CREB1, MAP2, NTRK2 mRNAs, as well as to GRIN1 and KPNB1 mRNAs, but not to rRNAs (PubMed : 17210633).

See full target information CAPRIN1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 301:110418 PubMed40570961

2025

Functional investigation of the RNA helicase MOV10 with respect to its interplay with factors involved in nonsense-mediated mRNA decay.

Applications

Unspecified application

Species

Unspecified reactive species

Guangpu Xue,Gabriel P Faber,Lea S Pommerening,Megha Mallick,Aditi Gupta,Markus C Wahl,Yaron Shav-Tal,Sutapa Chakrabarti

Nucleic acids research 52:5356-5375 PubMed38366783

2024

Nuclear RNA-related processes modulate the assembly of cytoplasmic RNA granules.

Applications

Unspecified application

Species

Unspecified reactive species

Mor Angel,Eden Fleshler,Mohammad Khaled Atrash,Noa Kinor,Jennifer I C Benichou,Yaron Shav-Tal

Neoplasma 69:832-840 PubMed35532296

2022

LIMD2 promotes tumor proliferation, invasion, and epithelial-mesenchymal transition in clear cell renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Qi-Yu Zhong,Ying-Wei Xie,Zhi-Liang Chen,Yu-Qing Chen,Yue-Xin Liu,Wen-Lian Xie,Wei Yan

View all publications

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Product promise

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Anti-Caprin-1 antibody

Western blot - Anti-Caprin-1 antibody (AB205377)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (3)

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