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AB313547

Anti-Caprin-1 antibody [RP23040261]

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Rabbit Recombinant Multiclonal Caprin-1 antibody. Suitable for ICC/IF, RIP and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CAPRIN1.

View Alternative Names

GPIAP1, GPIP137, M11S1, RNG105, CAPRIN1, Caprin-1, Cell cycle-associated protein 1, Cytoplasmic activation- and proliferation-associated protein 1, GPI-anchored membrane protein 1, GPI-anchored protein p137, Membrane component chromosome 11 surface marker 1, RNA granule protein 105, GPI-p137, p137GPI

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Caprin-1 antibody [RP23040261] (AB313547)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Caprin-1 antibody [RP23040261] (AB313547)

Immunofluorescence analysis of A549 cells (untransfected, panel a-d), transfected with GPIP137 specific siRNA (panel i-l) or non-specific scrambled siRNA (panels e-h) fixed using 4% formaldehyde (reconstituted in 1X PBS) for 10 min at room temperature and permeabilized using 0.1 % Triton X-100 in PBS for 15 min at room temperature labeling GPIP137 with ab313547 at 1/100 dilution followed by Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor? 488 conjugate (Product # A27034, 1/2000). Nuclei (blue) were stained using DAPI, and Rhodamine Phalloidin at 1/300 dilution was used for cytoskeletal F-actin (red) staining. Significant reduction of the signal was observed upon siRNA mediated knockdown (panel i-l) confirming the specificity of the antibody to GPIP137 green). The images were captured at 60X magnification.

Immunocytochemistry/ Immunofluorescence - Anti-Caprin-1 antibody [RP23040261] (AB313547)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Caprin-1 antibody [RP23040261] (AB313547)

Immunofluorescent analysis of MDA-MB-231 cells fixed using 4% formaldehyde (reconstituted in 1X PBS) for 10 min at room temperature and permeabilized using 0.1 % Triton X-100 in PBS for 15 min at room temperature labeling GPIP137 with ab313547 at 1/100 dilution followed by Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor? 488 conjugate at 1/2000 dilution. Panel a) shows representative cells that were stained for detection and localization of GPIP137 protein (green), Panel b) is stained for nuclei (blue) using DAPI. Panel c) represents cytoskeletal F-actin staining using Rhodamine Phalloidin at 1/300 dilution. Panel d) is a composite image of Panels a, b and c clearly demonstrating cytoplasmic localization of GPIP137. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

RIP - Anti-Caprin-1 antibody [RP23040261] (AB313547)
  • RIP

Supplier Data

RIP - Anti-Caprin-1 antibody [RP23040261] (AB313547)

GPIP137 was immunoprecipitated from whole cell lysates from 2 million HCT 116 cells with ab313547 at 4 µg. Normal Rabbit IgG was used as a negative IP control. RNA purified with a RNA Purification Kit was analyzed by RT-PCR using the Power SYBR? Green RNA-to-CT™ 1-Step Kit with RIP primer pairs over MYC, SIRT1, MAP1B, CCND2 mRNA (positive) and 18s rRNA (negative). Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

Key facts

Host species

Rabbit

Clonality

Multiclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

RIP, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human CAPRIN1.

Q14444

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "RIP" : {"fullname" : "RIP", "shortname":"RIP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "RIP-species-checked": "testedAndGuaranteed", "RIP-species-dilution-info": "2 µg for 10^6 Cells", "RIP-species-notes": "<p></p>" } } }
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Product details

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.09% Sodium azide Constituents: 99.91% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

mRNA-binding protein that acts as a regulator of mRNAs transport, translation and/or stability, and which is involved in neurogenesis, synaptic plasticity in neurons and cell proliferation and migration in multiple cell types (PubMed : 17210633, PubMed : 31439799, PubMed : 35979925). Plays an essential role in cytoplasmic stress granule formation (PubMed : 35977029). Acts as an mRNA regulator by mediating formation of some phase-separated membraneless compartment : undergoes liquid-liquid phase separation upon binding to target mRNAs, leading to assemble mRNAs into cytoplasmic ribonucleoprotein granules that concentrate mRNAs with associated regulatory factors (PubMed : 31439799, PubMed : 32302570, PubMed : 32302571, PubMed : 32302572, PubMed : 34074792, PubMed : 36040869, PubMed : 36279435). Undergoes liquid-liquid phase separation following phosphorylation and interaction with FMR1, promoting formation of cytoplasmic ribonucleoprotein granules that concentrate mRNAs with factors that inhibit translation and mediate deadenylation of target mRNAs (PubMed : 31439799). In these cytoplasmic ribonucleoprotein granules, CAPRIN1 mediates recruitment of CNOT7 deadenylase, leading to mRNA deadenylation and degradation (PubMed : 31439799). Binds directly and selectively to MYC and CCND2 mRNAs (PubMed : 17210633). In neuronal cells, directly binds to several mRNAs associated with RNA granules, including BDNF, CAMK2A, CREB1, MAP2, NTRK2 mRNAs, as well as to GRIN1 and KPNB1 mRNAs, but not to rRNAs (PubMed : 17210633).
See full target information CAPRIN1
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Product promise

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