Anti-CAPS2 antibody [EPR27004-72] - BSA and Azide free

11 product images

• 

  Western blot - Anti-CAPS2 antibody [EPR27004-72] - BSA and Azide free (ab307725)

  This data was developed using Anti-CAPS2 antibody [EPR27004-72] ab307724, the same antibody clone in a different buffer formulation.
  
  Blocking and diluting buffer and concentration: 5% NFDM/TBST.
  
  This antibody does not cross-react with human CAPS1.
  
  In Western blot, anti-His antibody (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution. Anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
  
  Exposure time: 6 seconds

  All lanes: Western blot - Anti-CAPS2 antibody [EPR27004-72] (Anti-CAPS2 antibody [EPR27004-72] ab307724) at 1/1000 dilution

  Lanes 1 - 2: HEK-293 transfected with a human CAPS2 (WT) expression vector containing a myc-His-tag® whole cell lysate at 20 µg

  Lane 3: HEK-293 transfected with a human CAPS1 (WT) expression vector containing a myc-His-tag® whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Observed band size: 150 kDa

  Exposure time: 6s

• 

  Western blot - Anti-CAPS2 antibody [EPR27004-72] - BSA and Azide free (ab307725)

  This data was developed using Anti-CAPS2 antibody [EPR27004-72] ab307724, the same antibody clone in a different buffer formulation.
  
  Blocking and diluting buffer and concentration: 5% NFDM/TBST
  
  The identity of the lower MW band at approximately 35 kDa is unknown.
  
  Exposure time:
  
  Lane 1: 103 seconds;
  Lane 2: 26 seconds.

  All lanes: Western blot - Anti-CAPS2 antibody [EPR27004-72] (Anti-CAPS2 antibody [EPR27004-72] ab307724) at 1/1000 dilution

  Lane 1: Human brain tissue lysate at 20 µg

  Lane 2: Human cerebellum tissue lysate at 20 µg

  Secondary

  All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

  Observed band size: 150 kDa

• 

  Western blot - Anti-CAPS2 antibody [EPR27004-72] - BSA and Azide free (ab307725)

  This data was developed using Anti-CAPS2 antibody [EPR27004-72] ab307724, the same antibody clone in a different buffer formulation.
  
  Blocking and diluting buffer and concentration: 5% NFDM/TBST
  
  Negative control: spleen (PMID: 14530279, 17428348).
  
  The identity of the lower MW band at approximately 35 kDa is unknown. In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
  
  Exposure time:
  Lanes 1-4: 37 seconds;
  Lanes 5-6: 92 seconds.

  All lanes: Western blot - Anti-CAPS2 antibody [EPR27004-72] (Anti-CAPS2 antibody [EPR27004-72] ab307724) at 1/1000 dilution

  Lane 1: Rat brain tissue lysate 20 μg at 20 µg

  Lane 2: Rat cerebellum tissue lysate 20 μg at 20 µg

  Lane 3: Rat spleen tissue lysate at 20 µg

  Lane 4: Mouse brain tissue lysate at 20 µg

  Lane 5: Mouse cerebellum tissue lysate at 20 µg

  Lane 6: Mouse spleen tissue lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  Observed band size: 150 kDa

• 

  Immunohistochemistry (Frozen sections) - Anti-CAPS2 antibody [EPR27004-72] - BSA and Azide free (ab307725)

  This data was developed using Anti-CAPS2 antibody [EPR27004-72] ab307724, the same antibody clone in a different buffer formulation.
  
  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat skeletal muscle (fresh) tissue labeling CAPS2 with Anti-CAPS2 antibody [EPR27004-72] ab307724 at 1/100 dilution (5.2 µg/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL) (Green).
  Negative control: confocal image showing no staining on rat skeletal muscle (PMID:14530279).
  The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-CAPS2 antibody [EPR27004-72] ab307724 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL).

• 

  Immunohistochemistry (Frozen sections) - Anti-CAPS2 antibody [EPR27004-72] - BSA and Azide free (ab307725)

  This data was developed using Anti-CAPS2 antibody [EPR27004-72] ab307724, the same antibody clone in a different buffer formulation.
  
  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat cardiac muscle (fresh) tissue labeling CAPS2 with Anti-CAPS2 antibody [EPR27004-72] ab307724 at 1/100 dilution (5.2 µg/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL) (Green).
  Negative control: confocal image showing no staining on rat cardiac muscle (PMID: 14530279).
  The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-CAPS2 antibody [EPR27004-72] ab307724 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL).

• 

  Immunohistochemistry (Frozen sections) - Anti-CAPS2 antibody [EPR27004-72] - BSA and Azide free (ab307725)

  This data was developed using Anti-CAPS2 antibody [EPR27004-72] ab307724, the same antibody clone in a different buffer formulation.
  
  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat spleen (fresh) tissue labeling CAPS2 with Anti-CAPS2 antibody [EPR27004-72] ab307724 at 1/100 dilution (5.2 µg/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL) (Green).
  Negative control: confocal image showing no staining on rat spleen (PMID: 14530279). 
  The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-CAPS2 antibody [EPR27004-72] ab307724 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL).

• 

  Immunohistochemistry (Frozen sections) - Anti-CAPS2 antibody [EPR27004-72] - BSA and Azide free (ab307725)

  This data was developed using Anti-CAPS2 antibody [EPR27004-72] ab307724, the same antibody clone in a different buffer formulation.
  
  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat cerebellum (fresh) tissue labeling CAPS2 with Anti-CAPS2 antibody [EPR27004-72] ab307724 at 1/100 dilution (5.2 µg/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL) (Green).
  Confocal image showing positive staining on rat cerebellum.
  The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-CAPS2 antibody [EPR27004-72] ab307724 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL).

• 

  Immunohistochemistry (Frozen sections) - Anti-CAPS2 antibody [EPR27004-72] - BSA and Azide free (ab307725)

  This data was developed using Anti-CAPS2 antibody [EPR27004-72] ab307724, the same antibody clone in a different buffer formulation.
  
  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse skeletal muscle (fresh) tissue labeling CAPS2 with Anti-CAPS2 antibody [EPR27004-72] ab307724 at 1/100 dilution (5.2 µg/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL) (Green).
  Negative control: confocal image showing no staining on mouse skeletal muscle (PMID:14530279).
  The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-CAPS2 antibody [EPR27004-72] ab307724 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL).

• 

  Immunohistochemistry (Frozen sections) - Anti-CAPS2 antibody [EPR27004-72] - BSA and Azide free (ab307725)

  This data was developed using Anti-CAPS2 antibody [EPR27004-72] ab307724, the same antibody clone in a different buffer formulation.
  
  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cardiac muscle (fresh) tissue labeling CAPS2 with Anti-CAPS2 antibody [EPR27004-72] ab307724 at 1/100 dilution (5.2 µg/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL) (Green).
  Negative control: confocal image showing no staining on mouse cardiac muscle (PMID: 14530279).
  The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-CAPS2 antibody [EPR27004-72] ab307724 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL).

• 

  Immunohistochemistry (Frozen sections) - Anti-CAPS2 antibody [EPR27004-72] - BSA and Azide free (ab307725)

  This data was developed using Anti-CAPS2 antibody [EPR27004-72] ab307724, the same antibody clone in a different buffer formulation.
  
  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse spleen (fresh) tissue labeling CAPS2 with Anti-CAPS2 antibody [EPR27004-72] ab307724 at 1/100 dilution (5.2 µg/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL) (Green).
  Negative control: confocal image showing no staining on mouse spleen (PMID: 14530279). 
  The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-CAPS2 antibody [EPR27004-72] ab307724 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL).

• 

  Immunohistochemistry (Frozen sections) - Anti-CAPS2 antibody [EPR27004-72] - BSA and Azide free (ab307725)

  This data was developed using Anti-CAPS2 antibody [EPR27004-72] ab307724, the same antibody clone in a different buffer formulation.
  
  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebellum (fresh) tissue labeling CAPS2 with Anti-CAPS2 antibody [EPR27004-72] ab307724 at 1/100 dilution (5.2 µg/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL) (Green).
  Confocal image showing positive staining on mouse cerebellum.
  The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-CAPS2 antibody [EPR27004-72] ab307724 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL).