AB234836

Anti-CAPZA1 antibody

Name: Anti-CAPZA1 antibody (ab234836) | Abcam
Brand: Abcam Limited
SKU: AB234836
Price: 525 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal CAPZA1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human F-actin-capping protein subunit alpha-1 aa 200 to C-terminus.

View Alternative Names

F-actin-capping protein subunit alpha-1, CapZ alpha-1, CAPZA1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody (AB234836)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody (AB234836)

Paraffin-embedded human testis tissue stained for CAPZA1 using ab234836 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-CAPZA1 antibody (AB234836)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CAPZA1 antibody (AB234836)

HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for CAPZA1 (Green) using ab234836 at 1/100 dilution in ICC/IF. Secondary used is an Alexa-Fluor^®488-conjugated Goat Anti-Rabbit IgG (H+L).

Supplier Data

Western blot - Anti-CAPZA1 antibody (AB234836)

All lanes:

Western blot - Anti-CAPZA1 antibody (ab234836) at 1/500 dilution

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 2:

HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate

Lane 3:

NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate

Lane 4:

THP-1 (Human monocytic leukemia cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 33 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human F-actin-capping protein subunit alpha-1 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P52907

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purity >95%.

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CAPZA1 also known as F-actin-capping protein subunit alpha-1 is a critical actin-binding protein with a molecular mass of approximately 33 kDa. It plays a role in the regulation of actin filament dynamics by capping the barbed ends of actin filaments effectively preventing further polymerization and depolymerization. CAPZA1 is expressed in a wide variety of tissues including skeletal muscle heart and brain indicating its importance in various cellular processes.

Biological function summary

The function of CAPZA1 influences the regulation of actin cytoskeleton organization. It is a part of the heterodimeric complex known as the F-actin-capping protein which includes beta subunit counterparts like CAPZB. This complex helps maintain the stability of actin filaments which are critical for cell motility shape and intracellular transport. CAPZA1's role in these cellular functions highlights its importance in processes ranging from muscle contraction to neuronal growth.

Pathways

CAPZA1 contributes significantly to the actin cytoskeleton pathway partnering with proteins such as gelsolin and profilin. Its activity affects cytoskeletal rearrangements which are integral to fundamental actions like cellular signaling adhesion and migration. The protein interacts in the Wnt signaling pathway where it may influence cell proliferation and differentiation processes through its modulation of actin filament dynamics.

CAPZA1 is linked to conditions like cardiac hypertrophy and certain cancers owing to its regulatory effect on actin filament dynamics. The disruptions in CAPZA1 expression can lead to aberrant cytoskeletal organization and cellular activity. Interactions with proteins such as vinculin are noted in these pathologies particularly as they relate to cell adhesion and migration in cancer metastasis. Understanding CAPZA1's role could provide insights into therapeutic targets for these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments. May play a role in the formation of epithelial cell junctions (PubMed : 22891260). Forms, with CAPZB, the barbed end of the fast growing ends of actin filaments in the dynactin complex and stabilizes dynactin structure. The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity).

See full target information F-actin-capping protein subunit alpha-1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The American journal of pathology 193:1156-1169 PubMed37263345

2023

Development of Scaffold-Free Three-Dimensional Cholangiocyte Organoids to Study the Progression of Primary Sclerosing Cholangitis.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjun Zhang,Konstantina Kyritsi,Abdulkadir Isidan,Yujin Park,Ping Li,Arthur A Cross-Najafi,Kevin Lopez,Lindsey Kennedy,Keisaku Sato,Shannon Glaser,Heather Francis,Gianfranco Alpini,Burcin Ekser

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CAPZA1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody (AB234836)

Immunocytochemistry/ Immunofluorescence - Anti-CAPZA1 antibody (AB234836)

Western blot - Anti-CAPZA1 antibody (AB234836)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise