Rabbit Recombinant Monoclonal CAPZA1 antibody. Carrier free. Suitable for IHC-P, IP, WB and reacts with Human samples.
Images
![Western blot - Anti-CAPZA1 antibody [EPR11210-4R] - BSA and Azide free (AB249883), expandable thumbnail](https://content.abcam.com/products/images/capza1-antibody-epr11210-4r-bsa-and-azide-free-ab249883--western-blot-img116833.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CAPZA1 antibody [EPR11210-4R] - BSA and Azide free (AB249883)")
![Immunoprecipitation - Anti-CAPZA1 antibody [EPR11210-4R] - BSA and Azide free (AB249883), expandable thumbnail](https://content.abcam.com/products/images/capza1-antibody-epr11210-4r-bsa-and-azide-free-ab249883--immunoprecipitation-img77703.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-CAPZA1 antibody [EPR11210-4R] - BSA and Azide free (AB249883)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210-4R] - BSA and Azide free (AB249883), expandable thumbnail](https://content.abcam.com/products/images/capza1-antibody-epr11210-4r-bsa-and-azide-free-ab249883--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img138122.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210-4R] - BSA and Azide free (AB249883)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210-4R] - BSA and Azide free (AB249883), expandable thumbnail](https://content.abcam.com/products/images/capza1-antibody-epr11210-4r-bsa-and-azide-free-ab249883--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137085.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210-4R] - BSA and Azide free (AB249883)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | Flow Cyt | WB | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Tested | Not recommended |
| Mouse | Predicted | Predicted | Not recommended | Predicted | Not recommended |
| Rat | Predicted | Predicted | Not recommended | Predicted | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments. May play a role in the formation of epithelial cell junctions (PubMed:22891260). Forms, with CAPZB, the barbed end of the fast growing ends of actin filaments in the dynactin complex and stabilizes dynactin structure. The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity).
Alternative names
F-actin-capping protein subunit alpha-1, CapZ alpha-1, CAPZA1
Recommended products
Rabbit Recombinant Monoclonal CAPZA1 antibody. Carrier free. Suitable for IHC-P, IP, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR11210-4R
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab249883 is the carrier-free version of Anti-CAPZA1 antibody [EPR11210-4R] ab175233.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CAPZA1 also known as F-actin-capping protein subunit alpha-1 is a critical actin-binding protein with a molecular mass of approximately 33 kDa. It plays a role in the regulation of actin filament dynamics by capping the barbed ends of actin filaments effectively preventing further polymerization and depolymerization. CAPZA1 is expressed in a wide variety of tissues including skeletal muscle heart and brain indicating its importance in various cellular processes.
- Biological function summary
The function of CAPZA1 influences the regulation of actin cytoskeleton organization. It is a part of the heterodimeric complex known as the F-actin-capping protein which includes beta subunit counterparts like CAPZB. This complex helps maintain the stability of actin filaments which are critical for cell motility shape and intracellular transport. CAPZA1’s role in these cellular functions highlights its importance in processes ranging from muscle contraction to neuronal growth.
- Pathways
CAPZA1 contributes significantly to the actin cytoskeleton pathway partnering with proteins such as gelsolin and profilin. Its activity affects cytoskeletal rearrangements which are integral to fundamental actions like cellular signaling adhesion and migration. The protein interacts in the Wnt signaling pathway where it may influence cell proliferation and differentiation processes through its modulation of actin filament dynamics.
- Associated diseases and disorders
CAPZA1 is linked to conditions like cardiac hypertrophy and certain cancers owing to its regulatory effect on actin filament dynamics. The disruptions in CAPZA1 expression can lead to aberrant cytoskeletal organization and cellular activity. Interactions with proteins such as vinculin are noted in these pathologies particularly as they relate to cell adhesion and migration in cancer metastasis. Understanding CAPZA1's role could provide insights into therapeutic targets for these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-CAPZA1 antibody [EPR11210-4R] - BSA and Azide free (ab249883), expandable thumbnail](https://content.abcam.com/products/images/capza1-antibody-epr11210-4r-bsa-and-azide-free-ab249883--western-blot-img116833.jpg "Western blot - Anti-CAPZA1 antibody [EPR11210-4R] - BSA and Azide free (ab249883)")
Western blot - Anti-CAPZA1 antibody [EPR11210-4R] - BSA and Azide free (ab249883)
This data was developed using Anti-CAPZA1 antibody [EPR11210-4R] ab175233, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-CAPZA1 antibody [EPR11210-4R] (Anti-CAPZA1 antibody [EPR11210-4R] ab175233) at 1/10000 dilution
Lane 1: HeLa cell lysate at 10 µg
Lane 2: Jurkat cell lysate at 10 µg
Lane 3: 293T cell lysate at 10 µg
Lane 4: HepG2 cell lysate at 10 µg
Predicted band size: 33 kDa
![Immunoprecipitation - Anti-CAPZA1 antibody [EPR11210-4R] - BSA and Azide free (ab249883), expandable thumbnail](https://content.abcam.com/products/images/capza1-antibody-epr11210-4r-bsa-and-azide-free-ab249883--immunoprecipitation-img77703.jpg "Immunoprecipitation - Anti-CAPZA1 antibody [EPR11210-4R] - BSA and Azide free (ab249883)")
Immunoprecipitation - Anti-CAPZA1 antibody [EPR11210-4R] - BSA and Azide free (ab249883)
This data was developed using Anti-CAPZA1 antibody [EPR11210-4R] ab175233, the same antibody clone in a different buffer formulation.Immunoprecipitation of CAPZA1 from HepG2 cell lysate using Anti-CAPZA1 antibody [EPR11210-4R] ab175233 at 1/10.
All lanes: Immunoprecipitation - Anti-CAPZA1 antibody [EPR11210-4R] (Anti-CAPZA1 antibody [EPR11210-4R] ab175233)
Predicted band size: 33 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210-4R] - BSA and Azide free (ab249883), expandable thumbnail](https://content.abcam.com/products/images/capza1-antibody-epr11210-4r-bsa-and-azide-free-ab249883--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img138122.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210-4R] - BSA and Azide free (ab249883)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210-4R] - BSA and Azide free (ab249883)
This data was developed using Anti-CAPZA1 antibody [EPR11210-4R] ab175233, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human muscle tissue labeling CAPZA1 with Anti-CAPZA1 antibody [EPR11210-4R] ab175233 at 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210-4R] - BSA and Azide free (ab249883), expandable thumbnail](https://content.abcam.com/products/images/capza1-antibody-epr11210-4r-bsa-and-azide-free-ab249883--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137085.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210-4R] - BSA and Azide free (ab249883)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210-4R] - BSA and Azide free (ab249883)
This data was developed using Anti-CAPZA1 antibody [EPR11210-4R] ab175233, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human urinary bladder carcinoma tissue labeling CAPZA1 with Anti-CAPZA1 antibody [EPR11210-4R] ab175233 at 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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