AB249376

Anti-CAPZA1 antibody [EPR11210] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal CAPZA1 antibody. Carrier free. Suitable for IHC-P, IP, WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

F-actin-capping protein subunit alpha-1, CapZ alpha-1, CAPZA1

9 Images

Flow Cytometry (Intracellular) - Anti-CAPZA1 antibody [EPR11210] - BSA and Azide free (AB249376)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-CAPZA1 antibody [EPR11210] - BSA and Azide free (AB249376)

This data was developed using ab166892, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized Jurkat cells using ab166892 at a dilution of 1/10 (red) compared to a rabbit IgG (negative) (green).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210] - BSA and Azide free (AB249376)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210] - BSA and Azide free (AB249376)

This data was developed using ab166892, the same antibody clone in a different buffer formulation.

ab166892 showing +ve staining in Human urinary bladder carcinoma.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210] - BSA and Azide free (AB249376)

This data was developed using ab166892, the same antibody clone in a different buffer formulation.

ab166892 showing +ve staining in Human thyroid gland carcinoma.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210] - BSA and Azide free (AB249376)

This data was developed using ab166892, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human muscle tissue labeling CAPZA1 with ab166892 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210] - BSA and Azide free (AB249376)

This data was developed using ab166892, the same antibody clone in a different buffer formulation.

ab166892 showing +ve staining in Human colonic adenocarcinoma.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210] - BSA and Azide free (AB249376)

This data was developed using ab166892, the same antibody clone in a different buffer formulation.

ab166892 showing +ve staining in Human normal brain.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210] - BSA and Azide free (AB249376)

This data was developed using ab166892, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue labeling CAPZA1 with ab166892 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Immunoprecipitation - Anti-CAPZA1 antibody [EPR11210] - BSA and Azide free (AB249376)

This data was developed using ab166892, the same antibody clone in a different buffer formulation.ab166892, at 1/1000 dilution, staining CAPZA1 in HepG2 cell lysate immunoprecipitated using ab166892 at 1/10.

All lanes:

Immunoprecipitation - Anti-CAPZA1 + CAPZA2 antibody [EPR11210] (<a href='/en-us/products/primary-antibodies/capza1-antibody-epr11210-ab166892'>ab166892</a>)

Predicted band size: 33 kDa

false

Unknown

Western blot - Anti-CAPZA1 antibody [EPR11210] - BSA and Azide free (AB249376)

This data was developed using ab166892, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-CAPZA1 + CAPZA2 antibody [EPR11210] (<a href='/en-us/products/primary-antibodies/capza1-antibody-epr11210-ab166892'>ab166892</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Lane 4:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 33 kDa

false

Unconjugated

Anti-CAPZA1 + CAPZA2 antibody [EPR11210]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11210

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Flow Cyt (Intra), IP, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

ab249376 is the carrier-free version of ab166892.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CAPZA1 also known as F-actin-capping protein subunit alpha-1 is a critical actin-binding protein with a molecular mass of approximately 33 kDa. It plays a role in the regulation of actin filament dynamics by capping the barbed ends of actin filaments effectively preventing further polymerization and depolymerization. CAPZA1 is expressed in a wide variety of tissues including skeletal muscle heart and brain indicating its importance in various cellular processes.

Biological function summary

The function of CAPZA1 influences the regulation of actin cytoskeleton organization. It is a part of the heterodimeric complex known as the F-actin-capping protein which includes beta subunit counterparts like CAPZB. This complex helps maintain the stability of actin filaments which are critical for cell motility shape and intracellular transport. CAPZA1’s role in these cellular functions highlights its importance in processes ranging from muscle contraction to neuronal growth.

Pathways

CAPZA1 contributes significantly to the actin cytoskeleton pathway partnering with proteins such as gelsolin and profilin. Its activity affects cytoskeletal rearrangements which are integral to fundamental actions like cellular signaling adhesion and migration. The protein interacts in the Wnt signaling pathway where it may influence cell proliferation and differentiation processes through its modulation of actin filament dynamics.

CAPZA1 is linked to conditions like cardiac hypertrophy and certain cancers owing to its regulatory effect on actin filament dynamics. The disruptions in CAPZA1 expression can lead to aberrant cytoskeletal organization and cellular activity. Interactions with proteins such as vinculin are noted in these pathologies particularly as they relate to cell adhesion and migration in cancer metastasis. Understanding CAPZA1's role could provide insights into therapeutic targets for these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments. May play a role in the formation of epithelial cell junctions (PubMed : 22891260). Forms, with CAPZB, the barbed end of the fast growing ends of actin filaments in the dynactin complex and stabilizes dynactin structure. The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity).

See full target information F-actin-capping protein subunit alpha-1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CAPZA1 antibody [EPR11210] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-CAPZA1 antibody [EPR11210] - BSA and Azide free (AB249376)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210] - BSA and Azide free (AB249376)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210] - BSA and Azide free (AB249376)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210] - BSA and Azide free (AB249376)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210] - BSA and Azide free (AB249376)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210] - BSA and Azide free (AB249376)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210] - BSA and Azide free (AB249376)

Immunoprecipitation - Anti-CAPZA1 antibody [EPR11210] - BSA and Azide free (AB249376)

Western blot - Anti-CAPZA1 antibody [EPR11210] - BSA and Azide free (AB249376)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise