Rabbit Polyclonal CAPZA2 antibody. Suitable for WB and reacts with Mouse, Zebrafish, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human F-actin-capping protein subunit alpha-2 aa 100 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human F-actin-capping protein subunit alpha-2 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P47755
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Tested |
| Rat | Predicted |
| Cow | Predicted |
| Dog | Predicted |
| Monkey | Predicted |
| Zebrafish | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Zebrafish | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow, Dog, Monkey | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments.
Alternative names
F-actin-capping protein subunit alpha-2, CapZ alpha-2, CAPZA2
Recommended products
Rabbit Polyclonal CAPZA2 antibody. Suitable for WB and reacts with Mouse, Zebrafish, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human F-actin-capping protein subunit alpha-2 aa 100 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human F-actin-capping protein subunit alpha-2 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P47755
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CAPZA2 also known as capping protein (actin filament) muscle Z-line alpha 2 is a protein that mechanically functions to regulate actin filament dynamics by binding to the barbed ends. This protein has an approximate molecular mass of 33 kDa. CAPZA2 is predominantly expressed in muscle tissues suggesting its significant role in muscle function. The protein forms part of the actin capping protein complex specifically interacting with the CAPZB subunit.
- Biological function summary
CAPZA2 controls the growth of actin filaments an essential process for cell motility and stability. It is part of the capping protein complex which is necessary for the capping and stabilization of the fast-growing ends of actin filaments. This stabilization prevents further polymerization maintaining optimal filament length for cellular dynamics. CAPZA2 also plays a role in cytoskeletal organization important for muscle contraction and cell shape maintenance.
- Pathways
The actin-regulating activity of CAPZA2 integrates into pathways involving actin cytoskeleton organization and dynamics. It is significantly involved in the actin-mediated processes interacting with proteins such as Arp2/3 complex which initiates actin branching. CAPZA2's role in cytoskeleton assembly also positions it within the signaling pathways related to cell growth and muscle function impacting interactions with proteins like tropomyosin which stabilizes actin filaments.
- Associated diseases and disorders
CAPZA2 shows a connection to muscular disorders including myopathy. Dysfunction in actin filament regulation can lead to issues in muscle fiber integrity and function contributing to muscle weakness or degenerative conditions. CAPZA2 is also linked to cardiac disorders due to its important role in muscle contractility interacting with proteins like myosin which is vital for heart muscle function. Understanding CAPZA2's roles can aid in developing therapeutic targets for muscle-related diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-CAPZA2 antibody (ab101451)
12% SDS PAGE
All lanes: Western blot - Anti-CAPZA2 antibody (ab101451) at 1/1000 dilution
Lane 1: HepG2 whole cell lysate at 30 µg
Lane 2: HCT116 whole cell lysate at 30 µg
Predicted band size: 33 kDa
Western blot - Anti-CAPZA2 antibody (ab101451)
12% SDS PAGE
All lanes: Western blot - Anti-CAPZA2 antibody (ab101451) at 1/1000 dilution
All lanes: Adult zebrafish whole cell lysate at 10 µg
Predicted band size: 112 kDa, 29 kDa, 33 kDa, 34 kDa, 35 kDa, 94 kDa
Western blot - Anti-CAPZA2 antibody (ab101451)
12% SDS PAGE
All lanes: Western blot - Anti-CAPZA2 antibody (ab101451) at 1/1000 dilution
All lanes: NIH-3T3 whole cell lysate at 30 µg
Predicted band size: 33 kDa
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