AB101451

Anti-CAPZA2 antibody

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(1 Publication)

Rabbit Polyclonal CAPZA2 antibody. Suitable for WB and reacts with Mouse, Zebrafish, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human F-actin-capping protein subunit alpha-2 aa 100 to C-terminus.

View Alternative Names

F-actin-capping protein subunit alpha-2, CapZ alpha-2, CAPZA2

3 Images

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Western blot - Anti-CAPZA2 antibody (AB101451)

12% SDS PAGE

All lanes:

Western blot - Anti-CAPZA2 antibody (ab101451) at 1/1000 dilution

Lane 1:

HepG2 whole cell lysate at 30 µg

Lane 2:

HCT116 whole cell lysate at 30 µg

Predicted band size: 33 kDa

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Unknown

Western blot - Anti-CAPZA2 antibody (AB101451)

12% SDS PAGE

All lanes:

Western blot - Anti-CAPZA2 antibody (ab101451) at 1/1000 dilution

All lanes:

NIH-3T3 whole cell lysate at 30 µg

Predicted band size: 33 kDa

false

Unknown

Western blot - Anti-CAPZA2 antibody (AB101451)

12% SDS PAGE

All lanes:

Western blot - Anti-CAPZA2 antibody (ab101451) at 1/1000 dilution

All lanes:

Adult zebrafish whole cell lysate at 10 µg

Predicted band size: 112 kDa,29 kDa,33 kDa,34 kDa,35 kDa,94 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human, Zebrafish

Applications

applications

Immunogen

Recombinant Fragment Protein within Human F-actin-capping protein subunit alpha-2 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P47755

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CAPZA2 also known as capping protein (actin filament) muscle Z-line alpha 2 is a protein that mechanically functions to regulate actin filament dynamics by binding to the barbed ends. This protein has an approximate molecular mass of 33 kDa. CAPZA2 is predominantly expressed in muscle tissues suggesting its significant role in muscle function. The protein forms part of the actin capping protein complex specifically interacting with the CAPZB subunit.

Biological function summary

CAPZA2 controls the growth of actin filaments an essential process for cell motility and stability. It is part of the capping protein complex which is necessary for the capping and stabilization of the fast-growing ends of actin filaments. This stabilization prevents further polymerization maintaining optimal filament length for cellular dynamics. CAPZA2 also plays a role in cytoskeletal organization important for muscle contraction and cell shape maintenance.

Pathways

The actin-regulating activity of CAPZA2 integrates into pathways involving actin cytoskeleton organization and dynamics. It is significantly involved in the actin-mediated processes interacting with proteins such as Arp2/3 complex which initiates actin branching. CAPZA2's role in cytoskeleton assembly also positions it within the signaling pathways related to cell growth and muscle function impacting interactions with proteins like tropomyosin which stabilizes actin filaments.

CAPZA2 shows a connection to muscular disorders including myopathy. Dysfunction in actin filament regulation can lead to issues in muscle fiber integrity and function contributing to muscle weakness or degenerative conditions. CAPZA2 is also linked to cardiac disorders due to its important role in muscle contractility interacting with proteins like myosin which is vital for heart muscle function. Understanding CAPZA2's roles can aid in developing therapeutic targets for muscle-related diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments.

See full target information F-actin-capping protein subunit alpha-2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 30:3996-4002.e4 PubMed32209463

2020

Structural Basis for EPC1-Mediated Recruitment of MBTD1 into the NuA4/TIP60 Acetyltransferase Complex.

Applications

Unspecified application

Species

Unspecified reactive species

Heng Zhang,Maëva Devoucoux,Xiaosheng Song,Li Li,Gamze Ayaz,Harry Cheng,Wolfram Tempel,Cheng Dong,Peter Loppnau,Jacques Côté,Jinrong Min

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CAPZA2 antibody

Western blot - Anti-CAPZA2 antibody (AB101451)

Western blot - Anti-CAPZA2 antibody (AB101451)

Western blot - Anti-CAPZA2 antibody (AB101451)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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