AB175378

Anti-CAPZA2 antibody

Name: Anti-CAPZA2 antibody (ab175378) | Abcam
Brand: Abcam Limited
SKU: AB175378
Price: 520 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal CAPZA2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human F-actin-capping protein subunit alpha-2.

View Alternative Names

F-actin-capping protein subunit alpha-2, CapZ alpha-2, CAPZA2

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-CAPZA2 antibody (AB175378)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CAPZA2 antibody (AB175378)

Immunocytochemistry/Immunofluorescence analysis of HepG2 cells using ab175378. Blue DAPI for nuclear staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA2 antibody (AB175378)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA2 antibody (AB175378)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast cancer tissue labelling CAPZA2 with ab175378 at 1/200. Magnification : 400x.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA2 antibody (AB175378)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling CAPZA2 with ab175378 at 1/200. Magnification : 400x.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA2 antibody (AB175378)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung cancer tissue labelling CAPZA2 with ab175378 at 1/200. Magnification : 400x.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA2 antibody (AB175378)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue labelling CAPZA2 with ab175378 at 1/200. Magnification : 400x.

Supplier Data

Western blot - Anti-CAPZA2 antibody (AB175378)

All lanes:

Western blot - Anti-CAPZA2 antibody (ab175378) at 1/500 dilution

Lane 1:

HeLa lysate

Lane 2:

THP1 lysate

Lane 3:

22RV1 lysate

Lane 4:

Mouse brain lysate

Lane 5:

Mouse heart lysate

Predicted band size: 33 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human F-actin-capping protein subunit alpha-2.

P47755

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CAPZA2 also known as capping protein (actin filament) muscle Z-line alpha 2 is a protein that mechanically functions to regulate actin filament dynamics by binding to the barbed ends. This protein has an approximate molecular mass of 33 kDa. CAPZA2 is predominantly expressed in muscle tissues suggesting its significant role in muscle function. The protein forms part of the actin capping protein complex specifically interacting with the CAPZB subunit.

Biological function summary

CAPZA2 controls the growth of actin filaments an essential process for cell motility and stability. It is part of the capping protein complex which is necessary for the capping and stabilization of the fast-growing ends of actin filaments. This stabilization prevents further polymerization maintaining optimal filament length for cellular dynamics. CAPZA2 also plays a role in cytoskeletal organization important for muscle contraction and cell shape maintenance.

Pathways

The actin-regulating activity of CAPZA2 integrates into pathways involving actin cytoskeleton organization and dynamics. It is significantly involved in the actin-mediated processes interacting with proteins such as Arp2/3 complex which initiates actin branching. CAPZA2's role in cytoskeleton assembly also positions it within the signaling pathways related to cell growth and muscle function impacting interactions with proteins like tropomyosin which stabilizes actin filaments.

CAPZA2 shows a connection to muscular disorders including myopathy. Dysfunction in actin filament regulation can lead to issues in muscle fiber integrity and function contributing to muscle weakness or degenerative conditions. CAPZA2 is also linked to cardiac disorders due to its important role in muscle contractility interacting with proteins like myosin which is vital for heart muscle function. Understanding CAPZA2's roles can aid in developing therapeutic targets for muscle-related diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments.

See full target information F-actin-capping protein subunit alpha-2

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:1048 PubMed40659881

2025

Deciphering the role of CAPZA2 in neurodevelopmental disorders: insights from mouse models.

Applications

Unspecified application

Species

Unspecified reactive species

Mei Guo,Liming Liu,Xiao Mao,Manyu Xiao,Xiaobin He,Xing Pan,Yuewen Chen,Wanying Yi,Qibin Li,Xianglan Piao,Hua Wang,Yang Du,Yong Cheng

Cell death discovery 10:221 PubMed38719806

2024

Unique lipid composition maintained by extracellular blockade leads to prooncogenicity.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Kudo,Ryo Yanagiya,Masanori Hasegawa,Joaquim Carreras,Yoshimi Miki,Shunya Nakayama,Etsuko Nagashima,Yuji Miyatake,Kan Torii,Kiyoshi Ando,Naoya Nakamura,Akira Miyajima,Makoto Murakami,Ai Kotani

International journal of molecular sciences 20: PubMed31533312

2019

Freezing-Thawing Procedures Remodel the Proteome of Ram Sperm before and after In Vitro Capacitation.

Applications

Unspecified application

Species

Unspecified reactive species

Patricia Peris-Frau,Alicia Martín-Maestro,María Iniesta-Cuerda,Irene Sánchez-Ajofrín,Lourdes Mateos-Hernández,J Julián Garde,Margarita Villar,Ana Josefa Soler

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CAPZA2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-CAPZA2 antibody (AB175378)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA2 antibody (AB175378)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA2 antibody (AB175378)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA2 antibody (AB175378)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA2 antibody (AB175378)

Western blot - Anti-CAPZA2 antibody (AB175378)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Secondary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise