AB96618

Anti-CAPZB antibody

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(2 Publications)

Rabbit Polyclonal CAPZB antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human F-actin-capping protein subunit beta aa 1 to C-terminus.

View Alternative Names

F-actin-capping protein subunit beta, CapZ beta, CAPZB

1 Images

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Western blot - Anti-CAPZB antibody (AB96618)

12% SDS PAGE

All lanes:

Western blot - Anti-CAPZB antibody (ab96618) at 1/1000 dilution

All lanes:

H1299 whole cell lysate at 30 µg

Predicted band size: 31 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Recombinant Fragment Protein within Human F-actin-capping protein subunit beta aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P47756

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CAPZB also known as the capping protein beta subunit is an essential component of the actin cytoskeleton. It has a molecular mass of about 31 kDa and is expressed ubiquitously across various human tissues. CAPZB partners with CAPZA to form a heterodimeric complex called the capping protein which binds to the barbed ends of actin filaments. This binding is essential for regulating actin filament growth and stability by preventing the addition and loss of actin monomers at these ends.

Biological function summary

The capping of actin filaments by CAPZB helps maintain cellular structures and enables extensive processes like cell motility. CAPZB is an important part of the actin-capping activity within the capping protein complex. It directly affects processes such as muscle contraction cell migration and cell adhesion by modulating actin dynamics. Through these functions CAPZB impacts various cell types including muscle cells and neurons by ensuring proper assembly and function of actin networks.

Pathways

CAPZB plays a prominent role in the regulation of actin-associated pathways including the Arp2/3 complex pathway and the WAVE regulatory complex pathway. These pathways are involved in the control of actin nucleation and branching which are vital for reshaping the actin cytoskeleton in response to cellular signals. CAPZB interacts with proteins such as profilin and cofilin which are important for actin polymerization and depolymerization dynamics in these pathways.

CAPZB has shown links to cardiac hypertrophy and neurological disorders like Alzheimer's disease. Aberrations in CAPZB function can lead to disrupted actin dynamics affecting cardiac tissue structure and neuron morphology. In cardiac hypertrophy CAPZB's interactions with sarcomeric proteins like troponin are important while in Alzheimer's disease altered CAPZB function may connect with tau protein modifications impacting the cytoskeletal network within neurons.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments. Plays a role in the regulation of cell morphology and cytoskeletal organization. Forms, with CAPZB, the barbed end of the fast growing ends of actin filaments in the dynactin complex and stabilizes dynactin structure. The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity).

See full target information F-actin-capping protein subunit beta

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Animal bioscience 37:1558-1567 PubMed38754842

2024

Effect of cholesterol-loaded cyclodextrin treatment on boar sperm cryopreservation.

Applications

Unspecified application

Species

Unspecified reactive species

Silong Zhang,Hanbing Zhang,Kexiong Liu,Xiaoling Xu,Yusheng Qin,Linli Xiao,Chunmei Zhou,Jianliang Wu,Yan Liu,Jiahua Bai

International journal of molecular sciences 24: PubMed38139175

2023

PAICS/DYRK3 Multienzyme Interactions as Coregulators of Purinosome Formation and Metabolism on Radioresistance in Oral Squamous Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Chin-Sheng Huang,Ming-Shou Hsieh,Vijesh Kumar Yadav,Yang-Che Wu,Shao-Cheng Liu,Chi-Tai Yeh,Mao-Suan Huang

View all publications

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Product promise

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Anti-CAPZB antibody

Western blot - Anti-CAPZB antibody (AB96618)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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