Rabbit Polyclonal CAPZB antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CAPZB aa 1 to C-terminus.
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Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 59.99% PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human CAPZB aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P47756
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Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Cow | Predicted |
| Pig | Predicted |
| Zebrafish | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow, Pig, Zebrafish | Dilution info - | Notes - |
Associated Products
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Target data
Function
F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments. Plays a role in the regulation of cell morphology and cytoskeletal organization.
Alternative names
F-actin-capping protein subunit beta, CapZ beta, CAPZB
Recommended products
Rabbit Polyclonal CAPZB antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CAPZB aa 1 to C-terminus.
Alternative names
F-actin-capping protein subunit beta, CapZ beta, CAPZB
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 59.99% PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human CAPZB aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P47756
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- Activity summary
CAPZB also known as the capping protein beta subunit is an essential component of the actin cytoskeleton. It has a molecular mass of about 31 kDa and is expressed ubiquitously across various human tissues. CAPZB partners with CAPZA to form a heterodimeric complex called the capping protein which binds to the barbed ends of actin filaments. This binding is essential for regulating actin filament growth and stability by preventing the addition and loss of actin monomers at these ends.
- Biological function summary
The capping of actin filaments by CAPZB helps maintain cellular structures and enables extensive processes like cell motility. CAPZB is an important part of the actin-capping activity within the capping protein complex. It directly affects processes such as muscle contraction cell migration and cell adhesion by modulating actin dynamics. Through these functions CAPZB impacts various cell types including muscle cells and neurons by ensuring proper assembly and function of actin networks.
- Pathways
CAPZB plays a prominent role in the regulation of actin-associated pathways including the Arp2/3 complex pathway and the WAVE regulatory complex pathway. These pathways are involved in the control of actin nucleation and branching which are vital for reshaping the actin cytoskeleton in response to cellular signals. CAPZB interacts with proteins such as profilin and cofilin which are important for actin polymerization and depolymerization dynamics in these pathways.
- Associated diseases and disorders
CAPZB has shown links to cardiac hypertrophy and neurological disorders like Alzheimer's disease. Aberrations in CAPZB function can lead to disrupted actin dynamics affecting cardiac tissue structure and neuron morphology. In cardiac hypertrophy CAPZB's interactions with sarcomeric proteins like troponin are important while in Alzheimer's disease altered CAPZB function may connect with tau protein modifications impacting the cytoskeletal network within neurons.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-CAPZB antibody (ab96618)
12% SDS PAGE
All lanes: Western blot - Anti-CAPZB antibody (ab96618) at 1/1000 dilution
All lanes: H1299 whole cell lysate at 30 µg
Predicted band size: 31 kDa
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