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AB244309

Anti-Carbonic Anhydrase 12/CA12 antibody

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(1 Publication)

Rabbit Polyclonal CAH12 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CA12 aa 150-300.

View Alternative Names

Carbonic anhydrase 12, Carbonate dehydratase XII, Carbonic anhydrase XII, Tumor antigen HOM-RCC-3.1.3, CA-XII, CA12

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 12/CA12 antibody (AB244309)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 12/CA12 antibody (AB244309)

Formalin-fixed, paraffin-embedded human kidney tissue stained for Carbonic Anhydrase 12/CA12 using ab244309 at 1/200 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-Carbonic Anhydrase 12/CA12 antibody (AB244309)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Carbonic Anhydrase 12/CA12 antibody (AB244309)

PFA-fixed, Triton X-100 permeabilized RT4 (human urinary bladder cancer cell line) cells stained for Carbonic Anhydrase 12/CA12 (green) using ab244309 at 4 μg/ml in ICC/IF.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human CA12 aa 150-300. The exact immunogen used to generate this antibody is proprietary information.

O43570

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Reversible hydration of carbon dioxide.
See full target information CA12
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 25:11039-11052 PubMed34791807

2021

Pharmacological inhibition of Carbonic Anhydrase IX and XII to enhance targeting of acute myeloid leukaemia cells under hypoxic conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Fangli Chen,Emilia Licarete,Xue Wu,Daniela Petrusca,Callista Maguire,Max Jacobsen,Austyn Colter,George E Sandusky,Magdalena Czader,Maegan L Capitano,James P Ropa,H Scott Boswell,Fabrizio Carta,Claudiu T Supuran,Brian Parkin,Melissa L Fishel,Heiko Konig
View all publications
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Product promise

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