Name: Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal
SKU: ab195233
Rating: 4 (1 reviews)

Rabbit Recombinant Monoclonal CAH12 antibody. C-terminal. Suitable for WB, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 5 publications.

Images

Western blot - Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (AB195233), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	Flow Cyt (Intra)	IHC-P
Human	Tested	Tested	Tested

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/10000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/130	Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/50 - 1/100	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

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3 products for Alternative Product

Target data

See full target information Carbonic anhydrase 12

Function

Reversible hydration of carbon dioxide.

Alternative names

Carbonic anhydrase 12, Carbonate dehydratase XII, Carbonic anhydrase XII, Tumor antigen HOM-RCC-3.1.3, CA-XII, CA12

Recommended products

Rabbit Recombinant Monoclonal CAH12 antibody. C-terminal. Suitable for WB, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 5 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR14861
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

Western blot - Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (ab195233)
Lanes 1 - 3: Merged signal (red and green). Green - ab195233 observed at 40 kDa. Red - loading control Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.
ab195233 was shown to react with Carbonic Anhydrase 12/CA12 in wild-type A549 cells in Western blot with loss of signal observed in Ca12 knockout cell line Human CA12 knockout A549 cell line ab273738 (knockout cell lysate Human CA12 knockout A549 cell lysate ab273778). Wild-type A549 and Ca12 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween^®) before incubation with ab195233 and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
All lanes: Western blot - Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (ab195233) at 1/10000 dilution
Lane 1: Wild-type A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg
Lane 2: Ca12 knockout A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg
Lane 2: Western blot - Human CA12 knockout A549 cell line (Human CA12 knockout A549 cell line ab273738)
Lane 3: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 39 kDa
Observed band size: 40 kDa
Western blot - Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (ab195233)
All lanes: Western blot - Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (ab195233) at 1/10000 dilution
Lane 1: MCF7 cell lysate at 10 µg
Lanes 2 and 5: SKOV3 cell lysate at 10 µg
Lane 3: 293 cell lysate at 10 µg
Lane 4: HeLa cell lysate at 10 µg
Lane 6: Human fetal kidney lysate at 10 µg
Lane 7: Human ovary cancer lysate at 10 µg
Lane 8: Human breast cancer lysate at 10 µg
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 39 kDa
Exposure time: 10s
Flow Cytometry (Intracellular) - Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (ab195233)
Intracellular flow cytometric analysis of SK-OV-3cells (paraformaldehyde-fixed, 2%) labeling CA12 with ab195233 at 1/130 dilution (red) or a Rabbit monoclonal IgG (negative) (black), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution. Unlabeled cells (Blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (ab195233)
Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling CA12 with ab195233 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control).
Note: Cell membrane and cytoplasm staining on human stomach tissue was observed.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (ab195233)
Immunohistochemical analysis of paraffin-embedded human colon tissue labeling CA12 with ab195233 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control).
Note: Cell membrane and cytoplasm staining on human colon tissue was observed.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (ab195233)
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling CA12 with ab195233 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control).
Note: Cell membrane and weakly cytoplasm staining on human pancreas tissue was observed.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (ab195233)
Immunohistochemical analysis of paraffin-embedded Human bladder transitional cell carcinoma tissue labeling CA12 with ab195233 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control).
Note: Cell membrane and weakly cytoplasm staining on human transitional cell carcinoma of bladder tissue was observed.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (ab195233)
Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling CA12 with ab195233 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control).
Note: Cell membrane and weakly cytoplasm staining on human ovarian carcinoma tissue was observed.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (ab195233)
Immunohistochemical analysis of paraffin-embedded Human renal adenocarcinoma tissue labeling CA12 with ab195233 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control).
Note: Cell membrane and cytoplasm staining on human renal adenocarcinoma tissue was observed.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.