Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (AB195233)

Immunohistochemical analysis of paraffin-embedded Human bladder transitional cell carcinoma tissue labeling CA12 with ab195233 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset : negative control).

Note : Cell membrane and weakly cytoplasm staining on human transitional cell carcinoma of bladder tissue was observed.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (AB195233)

Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling CA12 with ab195233 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset : negative control).

Note : Cell membrane and cytoplasm staining on human stomach tissue was observed.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (AB195233)

Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling CA12 with ab195233 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset : negative control).

Note : Cell membrane and weakly cytoplasm staining on human ovarian carcinoma tissue was observed.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (AB195233)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling CA12 with ab195233 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset : negative control).

Note : Cell membrane and cytoplasm staining on human colon tissue was observed.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (AB195233)

Intracellular flow cytometric analysis of SK-OV-3cells (paraformaldehyde-fixed, 2%) labeling CA12 with ab195233 at 1/130 dilution (red) or a Rabbit monoclonal IgG (negative) (black), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution. Unlabeled cells (Blue).

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (AB195233)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling CA12 with ab195233 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset : negative control).

Note : Cell membrane and weakly cytoplasm staining on human pancreas tissue was observed.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (AB195233)

Immunohistochemical analysis of paraffin-embedded Human renal adenocarcinoma tissue labeling CA12 with ab195233 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset : negative control).

Note : Cell membrane and cytoplasm staining on human renal adenocarcinoma tissue was observed.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• WB

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Western blot - Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (AB195233)

All lanes:

Western blot - Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (ab195233) at 1/10000 dilution

Lane 1:

MCF7 cell lysate at 10 µg

Lanes 2 and 5:

SKOV3 cell lysate at 10 µg

Lane 3:

293 cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Lane 6:

Human fetal kidney lysate at 10 µg

Lane 7:

Human ovary cancer lysate at 10 µg

Lane 8:

Human breast cancer lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 39 kDa

true

Exposure time: 10s

• WB

Lab

Western blot - Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (AB195233)

Lanes 1 - 3 : Merged signal (red and green). Green - ab195233 observed at 40 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.

ab195233 was shown to react with Carbonic Anhydrase 12/CA12 in wild-type A549 cells in Western blot with loss of signal observed in Ca12 knockout cell line ab273738 (knockout cell lysate ab273778). Wild-type A549 and Ca12 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween^®) before incubation with ab195233 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (ab195233) at 1/10000 dilution

Lane 1:

Wild-type A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

Ca12 knockout A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

Western blot - Human CA12 knockout A549 cell line (<a href='/en-us/products/cell-lines/human-ca12-knockout-a549-cell-line-ab273738'>ab273738</a>)

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Predicted band size: 39 kDa

Observed band size: 40 kDa

false