Anti-Carbonic anhydrase 2/CA2 antibody [EPR5195]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic anhydrase 2/CA2 antibody [EPR5195] (AB124687)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human clear cell carcinoma tissue sections labeling Carbonic anhydrase 2/CA2 with purified ab124687 at 1/1600 (0.063 μg/ml). Heat mediated antigen retrieval was performed Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic anhydrase 2/CA2 antibody [EPR5195] (AB124687)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labeling Carbonic anhydrase 2/CA2 with purified ab124687 at 1/1600 (0.063 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic anhydrase 2/CA2 antibody [EPR5195] (AB124687)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kindey tissue sections labeling Carbonic anhydrase 2/CA2 with purified ab124687 at 1/1600 (0.063 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic anhydrase 2/CA2 antibody [EPR5195] (AB124687)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling Carbonic anhydrase 2/CA2 with Purified ab124687 at 1/1600 (0.063 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

• WB

Lab

Western blot - Anti-Carbonic anhydrase 2/CA2 antibody [EPR5195] (AB124687)

Lanes 1-4 : Merged signal (red and green). Green - ab124687 observed at 29 kDa. Red - loading control ab8245 observed at 36 kDa.

ab124687 Anti-Carbonic anhydrase 2/CA2 antibody [EPR5195] was shown to specifically react with Carbonic anhydrase 2/CA2 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab265072 (knockout cell lysate ab257084) was used. Wild-type and Carbonic anhydrase 2/CA2 knockout samples were subjected to SDS-PAGE. ab124687 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Carbonic anhydrase 2/CA2 antibody [EPR5195] (ab124687) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

CA2 knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human CA2 (Carbonic anhydrase 2) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-ca2-carbonic-anhydrase-2-knockout-hek-293t-cell-line-ab265072'>ab265072</a>)

Lane 3:

A431 cell lysate at 20 µg

Lane 4:

Caco-2 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 29 kDa

Observed band size: 29 kDa

false

• WB

Supplier Data

Western blot - Anti-Carbonic anhydrase 2/CA2 antibody [EPR5195] (AB124687)

All lanes:

Western blot - Anti-Carbonic anhydrase 2/CA2 antibody [EPR5195] (ab124687) at 1/5000 dilution

Lane 1:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 2:

Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

Human heart lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 29 kDa

Observed band size: 29 kDa

false

• WB

Lab

Western blot - Anti-Carbonic anhydrase 2/CA2 antibody [EPR5195] (AB124687)

Lane 1 : Wild-type HAP1 cell lysate (40 μg)
Lane 2 : Carbonic anhydrase 2/CA2 knockout HAP1 cell lysate (40 μg)
Lane 3 : A431 cell lysate (40 μg)
Lane 4 : HEK293 cell lysate (40 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab124687 observed at 32 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab124687 was shown to specifically react with Carbonic anhydrase 2/CA2 when Carbonic anhydrase 2/CA2 knockout samples were used. Wild-type and Carbonic anhydrase 2/CA2 knockout samples were subjected to SDS-PAGE. ab124687 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with IRDye^® 800CW Goat anti-Rabbit IgG (H + L) and IRDye® 680 Goat anti-Mouse IgG (H + L) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Carbonic anhydrase 2/CA2 antibody [EPR5195] (ab124687)

Predicted band size: 29 kDa

false

• WB

Supplier Data

Western blot - Anti-Carbonic anhydrase 2/CA2 antibody [EPR5195] (AB124687)

All lanes:

Western blot - Anti-Carbonic anhydrase 2/CA2 antibody [EPR5195] (ab124687) at 1/5000 dilution

Lane 1:

Mouse brain lysate at 20 µg

Lane 2:

Mouse heart lysate at 20 µg

Lane 3:

Rat brain lysate at 20 µg

Lane 4:

Rat spleen lysate at 20 µg

Lane 5:

Rat kidney lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 29 kDa

Observed band size: 29 kDa

false