Anti-Carbonic Anhydrase 4/CA4 antibody

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Carbonic Anhydrase 4/CA4 antibody (AB236315)

Immunofluorescence staining of Hela cells with CSB-PA004372LA01HU at 1 : 66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 4/CA4 antibody (AB236315)

Paraffin-embedded human liver cancer tissue stained for Carbonic Anhydrase 4/CA4 using ab236315 at 1/100 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.