Anti-Carbonic Anhydrase 4/CA4 antibody [EPR29192-88]

6 product images

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 4/CA4 antibody [EPR29192-88] (ab323257)

  Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Carbonic Anhydrase 4/CA4 with ab323257 at 1/2000 (0.25 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Negative control: No staining on human liver (PMID: 16260723).
  
  The section was incubated with ab323257 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 4/CA4 antibody [EPR29192-88] (ab323257)

  Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Carbonic Anhydrase 4/CA4 with ab323257 at 1/2000 (0.25 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on epithelium and endothelial cells of human colon (PMID: 18680595).
  
  The section was incubated with ab323257 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 4/CA4 antibody [EPR29192-88] (ab323257)

  Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Carbonic Anhydrase 4/CA4 with ab323257 at 1/2000 (0.25 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on endothelial cells of human cerebrum.
  
  The section was incubated with ab323257 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 4/CA4 antibody [EPR29192-88] (ab323257)

  Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling Carbonic Anhydrase 4/CA4 with ab323257 at 1/2000 (0.25 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on endothelial cells of human lung (PMID: 38314803).
  
  The section was incubated with ab323257 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Western blot - Anti-Carbonic Anhydrase 4/CA4 antibody [EPR29192-88] (ab323257)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  Low expression: liver(PMID: 16260723).

  The identity of the higher MW band at approximately 75 kDa (in lane 3) is unknown.

  Lanes 3-5 were developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

  In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

  Exposure time: Lanes 1-2 : 48 seconds; Lane 3: 114 seconds; Lanes 4-5: 48 seconds.

  All lanes: Western blot - Anti-Carbonic Anhydrase 4/CA4 antibody [EPR29192-88] (ab323257) at 1/1000 dilution

  Lane 1: Human lung tissue lysate at 30 µg

  Lane 2: Human liver tissue lysate at 30 µg

  Lane 3: Human colon tissue lysate at 30 µg

  Lane 4: Human tonsil tissue lysate at 20 µg

  Lane 5: Human kidney tissue lysate at 20 µg

  Secondary

  All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

  Observed band size: 35 kDa, 36 kDa

• 

  Western blot - Anti-Carbonic Anhydrase 4/CA4 antibody [EPR29192-88] (ab323257)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  Low expression: DLD-1(PMID: 26071132).

  Negative control: Jurkat.

  A band around 39 kDa in MCF7 and Jurkat was observed.

  The identity of the higher MW bands at approximately 50 kDa (in lane 5) are unknown.

  Lane 5 was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

  In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

  Exposure time: Lanes 1-4: 81 seconds; Lane 5: 59 seconds.

  All lanes: Western blot - Anti-Carbonic Anhydrase 4/CA4 antibody [EPR29192-88] (ab323257) at 1/1000 dilution

  Lane 1: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 30 µg

  Lane 2: HUVEC (human umbilical vein endothelial cell) whole cell lysate at 30 µg

  Lane 3: DLD-1 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 30 µg

  Lane 4: Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate at 30 µg

  Lane 5: Capan-1 (human pancreatic adenocarcinoma epithelial cell) whole cell lysate at 30 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  Observed band size: 35 kDa, 36 kDa