Rabbit Recombinant Monoclonal CAH6 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.
Images
![Western blot - Anti-Carbonic Anhydrase 6/CA6 antibody [EPR13954(2)] - BSA and Azide free (AB251516), expandable thumbnail](https://content.abcam.com/products/images/carbonic-anhydrase-6-ca6-antibody-epr139542-bsa-and-azide-free-ab251516--western-blot-img72609.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Carbonic Anhydrase 6/CA6 antibody [EPR13954(2)] - BSA and Azide free (AB251516)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 6/CA6 antibody [EPR13954(2)] - BSA and Azide free (AB251516), expandable thumbnail](https://content.abcam.com/products/images/carbonic-anhydrase-6-ca6-antibody-epr139542-bsa-and-azide-free-ab251516--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img99212.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 6/CA6 antibody [EPR13954(2)] - BSA and Azide free (AB251516)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 6/CA6 antibody [EPR13954(2)] - BSA and Azide free (AB251516), expandable thumbnail](https://content.abcam.com/products/images/carbonic-anhydrase-6-ca6-antibody-epr139542-bsa-and-azide-free-ab251516--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img115689.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 6/CA6 antibody [EPR13954(2)] - BSA and Azide free (AB251516)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target data
Function
Reversible hydration of carbon dioxide. Its role in saliva is unknown.
Alternative names
Carbonic anhydrase 6, Carbonate dehydratase VI, Carbonic anhydrase VI, Salivary carbonic anhydrase, Secreted carbonic anhydrase, CA-VI, CA6
Recommended products
Rabbit Recombinant Monoclonal CAH6 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR13954(2)
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab251516 is the carrier-free version of Anti-Carbonic Anhydrase 6/CA6 antibody [EPR13954(2)] ab210406.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-Carbonic Anhydrase 6/CA6 antibody [EPR13954(2)] - BSA and Azide free (ab251516), expandable thumbnail](https://content.abcam.com/products/images/carbonic-anhydrase-6-ca6-antibody-epr139542-bsa-and-azide-free-ab251516--western-blot-img72609.jpg "Western blot - Anti-Carbonic Anhydrase 6/CA6 antibody [EPR13954(2)] - BSA and Azide free (ab251516)")
Western blot - Anti-Carbonic Anhydrase 6/CA6 antibody [EPR13954(2)] - BSA and Azide free (ab251516)
This data was developed using Anti-Carbonic Anhydrase 6/CA6 antibody [EPR13954(2)] ab210406, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Carbonic Anhydrase 6/CA6 antibody [EPR13954(2)] (Anti-Carbonic Anhydrase 6/CA6 antibody [EPR13954(2)] ab210406) at 1/1000 dilution
Lane 1: Human parotid gland lysate at 10 µg
Lane 2: Human fetal kidney tissue lysate at 10 µg
Lane 3: Human fetal liver tissue lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 35 kDa
Observed band size: 35 kDa
Exposure time: 30s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 6/CA6 antibody [EPR13954(2)] - BSA and Azide free (ab251516), expandable thumbnail](https://content.abcam.com/products/images/carbonic-anhydrase-6-ca6-antibody-epr139542-bsa-and-azide-free-ab251516--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img99212.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 6/CA6 antibody [EPR13954(2)] - BSA and Azide free (ab251516)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 6/CA6 antibody [EPR13954(2)] - BSA and Azide free (ab251516)
This data was developed using Anti-Carbonic Anhydrase 6/CA6 antibody [EPR13954(2)] ab210406, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human parotid gland tissue labeling Carbonic Anhydrase 6/CA6 with Anti-Carbonic Anhydrase 6/CA6 antibody [EPR13954(2)] ab210406 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasmic staining on Human parotid gland is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 6/CA6 antibody [EPR13954(2)] - BSA and Azide free (ab251516), expandable thumbnail](https://content.abcam.com/products/images/carbonic-anhydrase-6-ca6-antibody-epr139542-bsa-and-azide-free-ab251516--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img115689.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 6/CA6 antibody [EPR13954(2)] - BSA and Azide free (ab251516)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 6/CA6 antibody [EPR13954(2)] - BSA and Azide free (ab251516)
This data was developed using Anti-Carbonic Anhydrase 6/CA6 antibody [EPR13954(2)] ab210406, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Carbonic Anhydrase 6/CA6 with Anti-Carbonic Anhydrase 6/CA6 antibody [EPR13954(2)] ab210406 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Negative staining on Human kidney. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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