Rabbit Recombinant Monoclonal CAH8 antibody. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CA8.
Images
![Western blot - Anti-Carbonic Anhydrase 8/CA8 antibody [005] (AB275656), expandable thumbnail](https://content.abcam.com/products/images/carbonic-anhydrase-8-ca8-antibody-005-ab275656--western-blot-img157560.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Carbonic Anhydrase 8/CA8 antibody [005] (AB275656)")
![Immunoprecipitation - Anti-Carbonic Anhydrase 8/CA8 antibody [005] (AB275656), expandable thumbnail](https://content.abcam.com/products/images/carbonic-anhydrase-8-ca8-antibody-005-ab275656--immunoprecipitation-img143533.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Carbonic Anhydrase 8/CA8 antibody [005] (AB275656)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human CA8. Database link P35219
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200 | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes - |
Target data
Function
Does not have a carbonic anhydrase catalytic activity.
Alternative names
CALS, CA8, Carbonic anhydrase-related protein, CARP, Carbonic anhydrase VIII, CA-VIII
Recommended products
Rabbit Recombinant Monoclonal CAH8 antibody. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CA8.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human CA8. Database link P35219
- Clone number
- 005
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-Carbonic Anhydrase 8/CA8 antibody [005] (ab275656), expandable thumbnail](https://content.abcam.com/products/images/carbonic-anhydrase-8-ca8-antibody-005-ab275656--western-blot-img157560.jpg "Western blot - Anti-Carbonic Anhydrase 8/CA8 antibody [005] (ab275656)")
Western blot - Anti-Carbonic Anhydrase 8/CA8 antibody [005] (ab275656)
Developed using the Odyssey technique.
All lanes: Western blot - Anti-Carbonic Anhydrase 8/CA8 antibody [005] (ab275656) at 1/500 dilution
Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg
Lane 2: A549 (human lung carcinoma cell line) whole cell lysate at 30 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 33 kDa
![Immunoprecipitation - Anti-Carbonic Anhydrase 8/CA8 antibody [005] (ab275656), expandable thumbnail](https://content.abcam.com/products/images/carbonic-anhydrase-8-ca8-antibody-005-ab275656--immunoprecipitation-img143533.jpg "Immunoprecipitation - Anti-Carbonic Anhydrase 8/CA8 antibody [005] (ab275656)")
Immunoprecipitation - Anti-Carbonic Anhydrase 8/CA8 antibody [005] (ab275656)
Carbonic Anhydrase 8 was immunoprecipitated from 0.5 mg of HEK-293T whole cell lysate (Lane A) or A549 whole cell lysate (Lane B) with 2 μL of ab275656 and 15 μL of 50% Protein G agarose. Western blot was performed from the immunoprecipitate using ab275656 at 1/200 dilution. Rabbit IgG (H+L) Dylight 800-labeled antibody, was used as secondary antibody at 1/5000 dilution.
Developed using the Odssey technique.All lanes: Immunoprecipitation - Anti-Carbonic Anhydrase 8/CA8 antibody [005] (ab275656)
Performed under reducing conditions.
Predicted band size: 33 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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