AB181464

Anti-Carbonic Anhydrase 9/CA9 antibody [10F7A8] - N-terminal

Name: Anti-Carbonic Anhydrase 9/CA9 antibody [10F7A8] - N-terminal (ab181464) | Abcam
Brand: Abcam Limited
SKU: AB181464
Price: 545 USD
Availability: InStock

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(1 Publication)

Mouse Monoclonal Carbonic Anhydrase 9 antibody. N-terminal. Suitable for Flow Cyt, WB and reacts with Human, Transfected cell lysate - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CA9 aa 1-200.

View Alternative Names

G250, MN, CA9, Carbonic anhydrase 9, Carbonate dehydratase IX, Carbonic anhydrase IX, Membrane antigen MN, P54/58N, Renal cell carcinoma-associated antigen G250, pMW1, CA-IX, CAIX, RCC-associated antigen G250

4 Images

Flow Cytometry - Anti-Carbonic Anhydrase 9/CA9 antibody [10F7A8] - N-terminal (AB181464)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-Carbonic Anhydrase 9/CA9 antibody [10F7A8] - N-terminal (AB181464)

Flow cytometric analysis of NTERA2 cells labeling Carbonic Anhydrase 9/CA9 with ab181464 at 1/200 dilution (green) and negative control (red).

Supplier Data

Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody [10F7A8] - N-terminal (AB181464)

All lanes:

Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody [10F7A8] - N-terminal (ab181464) at 1/500 dilution

Lane 1:

A431 cell line lysate

Lane 2:

SW620 cell line lysate

Predicted band size: 49 kDa

false

Supplier Data

Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody [10F7A8] - N-terminal (AB181464)

All lanes:

Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody [10F7A8] - N-terminal (ab181464) at 1/500 dilution

All lanes:

Carbonic Anhydrase 9/CA9 recombinant protein

Predicted band size: 49 kDa

false

Unknown

Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody [10F7A8] - N-terminal (AB181464)

All lanes:

Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody [10F7A8] - N-terminal (ab181464) at 1/500 dilution

Lane 1:

HEK293 cell lysate.

Lane 2:

Carbonic Anhydrase 9/CA9 (Amino acids: 37-186)-hIgGFc transfected HEK293 cell lysate

Predicted band size: 49 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

10F7A8

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

Flow Cyt, WB

applications

Immunogen

Recombinant Fragment Protein within Human CA9 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q16790

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "" }, "Transfected cell lysate - Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from tissue culture supernatant.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the interconversion between carbon dioxide and water and the dissociated ions of carbonic acid (i.e. bicarbonate and hydrogen ions).

See full target information CA9

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 7: PubMed35801590

2022

Abnormal lipid metabolism in epidermal Langerhans cells mediates psoriasis-like dermatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Xilin Zhang,Xiaorui Li,Yuanyuan Wang,Youdong Chen,Yijun Hu,Chunyuan Guo,Zengyang Yu,Peng Xu,Yangfeng Ding,Qing-Sheng Mi,Jianhua Wu,Jun Gu,Yuling Shi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Carbonic Anhydrase 9/CA9 antibody [10F7A8] - N-terminal

Flow Cytometry - Anti-Carbonic Anhydrase 9/CA9 antibody [10F7A8] - N-terminal (AB181464)

Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody [10F7A8] - N-terminal (AB181464)

Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody [10F7A8] - N-terminal (AB181464)

Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody [10F7A8] - N-terminal (AB181464)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

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