AB107257

Anti-Carbonic Anhydrase 9/CA9 antibody [2D3]

Name: Anti-Carbonic Anhydrase 9/CA9 antibody [2D3] (ab107257) | Abcam
Brand: Abcam Limited
SKU: AB107257
Price: 405 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(15 Publications)

Mouse Monoclonal Carbonic Anhydrase 9 antibody. Suitable for Flow Cyt, WB, IHC-P and reacts with Human samples. Cited in 15 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CA9.

View Alternative Names

G250, MN, CA9, Carbonic anhydrase 9, Carbonate dehydratase IX, Carbonic anhydrase IX, Membrane antigen MN, P54/58N, Renal cell carcinoma-associated antigen G250, pMW1, CA-IX, CAIX, RCC-associated antigen G250

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [2D3] (AB107257)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [2D3] (AB107257)

Immunohistochemical analysis of paraffin-embedded lung tissues (left) and colonic tissues (right) using 1/200 ab107257 with DAB staining.

Flow Cytometry - Anti-Carbonic Anhydrase 9/CA9 antibody [2D3] (AB107257)

Flow Cyt

Unknown

Flow Cytometry - Anti-Carbonic Anhydrase 9/CA9 antibody [2D3] (AB107257)

Flow cytometric analysis of NTERA-2 cells using Carbonic Anhydrase 9/CA9 mouse ab107257 in green and negative control in purple.

Unknown

Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody [2D3] (AB107257)

All lanes:

Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody [2D3] (ab107257) at 1/500 dilution

Lane 1:

Hela Cell lysate

Lane 2:

A549 Cell Lysate

Predicted band size: 49 kDa

false

Unconjugated

Anti-Hepatitis B Virus pre S2 Antigen antibody [2D3]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2D3

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

Flow Cyt, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human CA9. The exact immunogen used to generate this antibody is proprietary information.

Q16790

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "" } } }

style

left-column

Product details

This product was changed from ascites to supernatant. Lot no's high than GR311638-1 are from Tissue Culture Supernatant

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from tissue culture supernatant.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Catalyzes the interconversion between carbon dioxide and water and the dissociated ions of carbonic acid (i.e. bicarbonate and hydrogen ions).

See full target information CA9

style

left-column

Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Integrative biology : quantitative biosciences from nano to macro 15: PubMed37874173

2023

Human enteroids as a tool to study conventional and ultra-high dose rate radiation.

Applications

Unspecified application

Species

Unspecified reactive species

Katarina C Klett,Briana C Martin-Villa,Victoria S Villarreal,Stavros Melemenidis,Vignesh Viswanathan,Rakesh Manjappa,M Ramish Ashraf,Luis Soto,Brianna Lau,Suparna Dutt,Erinn B Rankin,Billy W Loo,Sarah C Heilshorn

Cancer letters 571:216338 PubMed37549770

2023

The CAIX inhibitor SLC-0111 exerts anti-cancer activity on gastric cancer cell lines and resensitizes resistant cells to 5-Fluorouracil, taxane-derived, and platinum-based drugs.

Applications

Unspecified application

Species

Unspecified reactive species

Elena Andreucci,Alessio Biagioni,Sara Peri,Giampaolo Versienti,Fabio Cianchi,Fabio Staderini,Lorenzo Antonuzzo,Claudiu T Supuran,Erika Olivo,Elisa Pasqualini,Luca Messerini,Daniela Massi,Matteo Lulli,Jessica Ruzzolini,Silvia Peppicelli,Francesca Bianchini,Nicola Schiavone,Lido Calorini,Lucia Magnelli,Laura Papucci

BMC biology 21:98 PubMed37106386

2023

Osteosarcoma tumors maintain intra-tumoral transcriptional heterogeneity during bone and lung colonization.

Applications

Unspecified application

Species

Unspecified reactive species

Sanjana Rajan,Emily M Franz,Camille A McAloney,Tatyana A Vetter,Maren Cam,Amy C Gross,Cenny Taslim,Meng Wang,Matthew V Cannon,Alexander Oles,Ryan D Roberts

Frontiers in oncology 13:1116648 PubMed36816933

2023

Clinical and pathological heterogeneity of four common fusion subtypes in Xp11.2 translocation renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Guo,Yiqi Zhu,Xiaohong Pu,Hongqian Guo,Weidong Gan

ACS omega 8:5787-5807 PubMed36816648

2023

Tail-Approach-Based Design and Synthesis of Coumarin-Monoterpenes as Carbonic Anhydrase Inhibitors and Anticancer Agents.

Applications

Unspecified application

Species

Unspecified reactive species

Belma Zengin Kurt,Gulsen Celebi,Dilek Ozturk Civelek,Andrea Angeli,Atilla Akdemir,Fatih Sonmez,Claudiu T Supuran

Cancer letters 528:76-84 PubMed34973392

2022

Proteolysis of a histone acetyl reader, ATAD2, induces chemoresistance of cancer cells under severe hypoxia by inhibiting cell cycle progression in S phase.

Applications

Unspecified application

Species

Unspecified reactive species

Takao Haitani,Minoru Kobayashi,Sho Koyasu,Shusuke Akamatsu,Tatsuya Suwa,Yasuhito Onodera,Jin-Min Nam,Phuong Thi Lien Nguyen,Toshi Menju,Hiroshi Date,Osamu Ogawa,Hiroshi Harada

Journal of enzyme inhibition and medicinal chemistry 36:954-963 PubMed33947294

2021

Anti-breast cancer action of carbonic anhydrase IX inhibitor 4-[4-(4-Benzo[1,3]dioxol-5-ylmethyl-piperazin-1-yl)-benzylidene-hydrazinocarbonyl]-benzenesulfonamide (BSM-0004): and studies.

Applications

Unspecified application

Species

Unspecified reactive species

Chandra Bhushan Mishra,Raj Kumar Mongre,Amresh Prakash,Raok Jeon,Claudiu T Supuran,Myeong-Sok Lee

Oncology reports 43:817-826 PubMed32020234

2020

Identification of anaplastic lymphoma kinase fusions in clear cell renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Chen,Wei Li,Bing Bai,Huafeng Wei

Cell reports 29:4086-4098.e6 PubMed31851935

2019

Mesothelial Cell HIF1α Expression Is Metabolically Downregulated by Metformin to Prevent Oncogenic Tumor-Stromal Crosstalk.

Applications

Unspecified application

Species

Unspecified reactive species

Peter C Hart,Hilary A Kenny,Niklas Grassl,Karen M Watters,Lacey M Litchfield,Fabian Coscia,Ivana Blaženović,Lisa Ploetzky,Oliver Fiehn,Matthias Mann,Ernst Lengyel,Iris L Romero

Experimental dermatology 28:976-980 PubMed31125456

2019

Carbonic anhydrases in human keratinocytes and their regulation by all-trans retinoic acid and 1α,25-dihydroxyvitamin D.

Applications

Unspecified application

Species

Unspecified reactive species

Bani Kaur Suri,Artur Schmidtchen,Navin Kumar Verma

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Carbonic Anhydrase 9/CA9 antibody [2D3]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [2D3] (AB107257)

Flow Cytometry - Anti-Carbonic Anhydrase 9/CA9 antibody [2D3] (AB107257)

Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody [2D3] (AB107257)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Product protocols

Target data

Publications (15)

Product promise