AB184006

Anti-Carbonic Anhydrase 9/CA9 antibody

Name: Anti-Carbonic Anhydrase 9/CA9 antibody (ab184006) | Abcam
Brand: Abcam Limited
SKU: AB184006
Rating: 4 (1 reviews)

(1 Review)

(19 Publications)

Rabbit Polyclonal Carbonic Anhydrase 9 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 19 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CA9 aa 1-200.

View Alternative Names

G250, MN, CA9, Carbonic anhydrase 9, Carbonate dehydratase IX, Carbonic anhydrase IX, Membrane antigen MN, P54/58N, Renal cell carcinoma-associated antigen G250, pMW1, CA-IX, CAIX, RCC-associated antigen G250

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody (AB184006)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody (AB184006)

Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling Carbonic Anhydrase 9/CA9 with ab184006 at 1/500 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody (AB184006)

Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue stained for Carbonic Anhydrase IX protein at cell membrane in human renal cell carcinoma with ab184006 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min.

Immunocytochemistry/ Immunofluorescence - Anti-Carbonic Anhydrase 9/CA9 antibody (AB184006)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Carbonic Anhydrase 9/CA9 antibody (AB184006)

Immunofluorescence analysis of A431 cells fixed in ice-cold MeOH for 5 min labelling Carbonic Anhydrase IX protein at cell membrane using ab184006 at a 1/500 dilution (Green). DAPI was used for nuclear staining (Blue).

Supplier Data

Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody (AB184006)

Samples were separated by 10 % SDS.

All lanes:

Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody (ab184006) at 1/1000 dilution

Lane 1:

Untreated HeLa whole cell extracts at 30 µg

Lane 2:

Treated HeLa whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 50 kDa

false

Supplier Data

Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody (AB184006)

Samples were separated by 7.5 % SDS.

All lanes:

Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody (ab184006) at 1/5000 dilution

Lane 1:

Untreated HeLa whole cell extracts at 30 µg

Lane 2:

Treated HeLa whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 50 kDa

false

Supplier Data

Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody (AB184006)

Samples were separated by 10 % SDS.

All lanes:

Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody (ab184006) at 1/1000 dilution

Lane 1:

Untreated HeLa whole cell extracts at 30 µg

Lane 2:

Treated HeLa whole cell extracts treated with 1% O2 for 4 hr at 30 µg

Lane 3:

HeLa whole cell extracts treated with 1% O2 for 24 hr at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 50 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human CA9 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q16790

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the interconversion between carbon dioxide and water and the dissociated ions of carbonic acid (i.e. bicarbonate and hydrogen ions).

See full target information CA9

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Publications (19)

Recent publications for all applications. Explore the full list and refine your search

International journal of medical sciences 22:2583-2593 PubMed40520896

2025

The combination of PF-429242 and chloroquine triggers pH-dependent cell death in hepatocellular carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jiunn-Chang Lin,Tun-Sung Huang,Yan-Bin Chen,Pao-Shu Wu,Tsang-Pai Liu,Pei-Ming Yang

Cancer science 115:1405-1416 PubMed38413363

2024

Hypoxia-related carbonic anhydrase 9 induces serpinB9 expression in cancer cells and apoptosis in T cells via acidosis.

Applications

Unspecified application

Species

Unspecified reactive species

Mamoru Harada,Hitoshi Kotani,Yuichi Iida,Ryosuke Tanino,Takafumi Minami,Yoshihiro Komohara,Kazuhiro Yoshikawa,Hirotsugu Uemura

iScience 27:108797 PubMed38303694

2024

A tumor endothelial cell-specific microRNA replacement therapy for hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hideki Iwamoto,Hiroyuki Suzuki,Atsutaka Masuda,Takahiko Sakaue,Toru Nakamura,Toshimitsu Tanaka,Miwa Sakai,Yasuko Imamura,Hirohisa Yano,Takuji Torimura,Hironori Koga,Kaori Yasuda,Masakatsu Tsurusaki,Takahiro Seki,Takumi Kawaguchi

Cell communication and signaling : CCS 22:15 PubMed38183060

2024

Hypoxia-induced ZEB1 promotes cervical cancer immune evasion by strengthening the CD47-SIRPα axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Jing Chen,Chu-Hong Guo,Zi-Ci Wang,Yang Yang,Yu-Hua Pan,Jie-Ying Liang,Mei-Ge Sun,Liang-Sheng Fan,Li Liang,Wei Wang

Frontiers in immunology 14:1245708 PubMed37795103

2023

Novel miRNA-based drug CD5-2 reduces liver tumor growth in diethylnitrosamine-treated mice by normalizing tumor vasculature and altering immune infiltrate.

Applications

Unspecified application

Species

Unspecified reactive species

Ken Liu,Jinbiao Chen,Yang Zhao,Jade Boland,Ka Ka Ting,Glen Lockwood,Catriona McKenzie,James Kench,Mathew A Vadas,Jennifer R Gamble,Geoffrey W McCaughan

iScience 26:106804 PubMed37235058

2023

Hypoxia-induced loss of SRSF2-dependent DNA methylation promotes CTCF-mediated alternative splicing of VEGFA in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Pooja Yadav,Anchala Pandey,Parik Kakani,Srinivas Abhishek Mutnuru,Atul Samaiya,Jharna Mishra,Sanjeev Shukla

Cancers 14: PubMed35892853

2022

Repositioning of Old Drugs for Novel Cancer Therapies: Continuous Therapeutic Perfusion of Aspirin and Oseltamivir Phosphate with Gemcitabine Treatment Disables Tumor Progression, Chemoresistance, and Metastases.

Applications

Unspecified application

Species

Unspecified reactive species

Bessi Qorri,Reza Bayat Mokhtari,William W Harless,Myron R Szewczuk

BMC immunology 23:20 PubMed35461243

2022

Macrophage-targeted anti-CCL2 immunotherapy enhances tumor sensitivity to 5-fluorouracil in a Balb/c-CT26 murine colon carcinoma model measured using diffuse reflectance spectroscopy.

Applications

Unspecified application

Species

Unspecified reactive species

Shelby N Bess,Gage J Greening,Narasimhan Rajaram,Timothy J Muldoon

Translational oncology 14:101201 PubMed34388691

2021

Efficacy and tolerability of Sorafenib plus metronomic chemotherapy S-1 for advanced hepatocellular carcinoma in preclinical and clinical assessments.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroyuki Suzuki,Hideki Iwamoto,Masahito Nakano,Toru Nakamura,Atsutaka Masuda,Takahiko Sakaue,Toshimitsu Tanaka,Dan Nakano,Ryoko Kuromatsu,Takashi Niizeki,Shusuke Okamura,Shigeo Shimose,Tomotake Shirono,Yu Noda,Naoki Kamachi,Hirohisa Yano,Atsushi Kawaguchi,Hironori Koga,Takuji Torimura

International journal of radiation oncology, biology, physics 110:1222-1233 PubMed33587991

2021

Tumor Oxygenation by Myo-Inositol Trispyrophosphate Enhances Radiation Response.

Applications

Unspecified application

Species

Unspecified reactive species

Ivo Grgic,Fabienne Tschanz,Nathalie Borgeaud,Anurag Gupta,Pierre-Alain Clavien,Matthias Guckenberger,Rolf Graf,Martin Pruschy

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Carbonic Anhydrase 9/CA9 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody (AB184006)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody (AB184006)

Immunocytochemistry/ Immunofluorescence - Anti-Carbonic Anhydrase 9/CA9 antibody (AB184006)

Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody (AB184006)

Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody (AB184006)

Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody (AB184006)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (19)

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