Rabbit Recombinant Monoclonal Carbonic Anhydrase 9 antibody. Suitable for IP, WB, IHC-P, IHC-Fr and reacts with Mouse, Rat, Human samples. Cited in 8 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (AB243660), expandable thumbnail](https://content.abcam.com/products/images/carbonic-anhydrase-9-ca9-antibody-epr23055-5-ab243660--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img58712.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (AB243660)")
![Immunohistochemistry (Frozen sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (AB243660), expandable thumbnail](https://content.abcam.com/products/images/carbonic-anhydrase-9-ca9-antibody-epr23055-5-ab243660--immunohistochemistry-frozen-sections-img64030.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (AB243660)")
![Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (AB243660), expandable thumbnail](https://content.abcam.com/products/images/carbonic-anhydrase-9-ca9-antibody-epr23055-5-ab243660--western-blot-img61287.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (AB243660)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (AB243660), expandable thumbnail](https://content.abcam.com/products/images/carbonic-anhydrase-9-ca9-antibody-epr23055-5-ab243660--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img117564.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (AB243660)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (AB243660), expandable thumbnail](https://content.abcam.com/products/images/carbonic-anhydrase-9-ca9-antibody-epr23055-5-ab243660--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img116113.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (AB243660)")
Publications
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- Clinical and translational medicine 13:e13832023Nme Cas9-mediated therapeutic editing in inhibiting angiogenesis after wet age-related macular degeneration onset.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSihui Hu,Yuxi Chen,Dongchun Xie,Kan Xu,Yunzhao Fu,Wei Chi,Haiying Liu,Junjiu HuangPubMed 37598400
- Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e22074902023Nanomedicines Reprogram Synovial Macrophages by Scavenging Nitric Oxide and Silencing CA9 in Progressive Osteoarthritis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi Yan,An Lu,Yun Dou,Zhen Zhang,Xiang-Yu Wang,Lin Zhai,Li-Ya Ai,Ming-Ze Du,Lin-Xia Jiang,Yuan-Jun Zhu,Yu-Jie Shi,Xiao-Yan Liu,Dong Jiang,Jian-Cheng WangPubMed 36748885
- Cell death & disease 14:302023Single-cell RNA-seq integrated with multi-omics reveals SERPINE2 as a target for metastasis in advanced renal cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWen-Jin Chen,Ke-Qin Dong,Xiu-Wu Pan,Si-Shun Gan,Da Xu,Jia-Xin Chen,Wei-Jie Chen,Wen-Yan Li,Yu-Qi Wang,Wang Zhou,Brian Rini,Xin-Gang CuiPubMed 36646679
- Cancer medicine 12:8415-84322022IGFL2-AS1-induced suppression of HIF-1α degradation promotes cell proliferation and invasion in colorectal cancer by upregulating CA9.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMengdi Qin,Qiang Liu,Wei Yang,Qiaofeng Wang,Zheng XiangPubMed 36537608
- Cancer biology & medicine 19:2022ENO1 expression and Erk phosphorylation in PDAC and their effects on tumor cell apoptosis in a hypoxic microenvironment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuizhi Sun,Jing Mo,Runfen Cheng,Fan Li,Yue Li,Yuhong Guo,Yanlei Li,Yanhui Zhang,Xiaoyu Bai,Yalei Wang,Xueyi Dong,Danfang Zhang,Jihui HaoPubMed 36476328
- Frontiers in physiology 13:8768902022Vitamin D Receptor-Dependent Protective Effect of Moderate Hypoxia in a Mouse Colitis Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZheng Wang,Hong Yang,Hong Lv,Changzhi Huang,Jiaming QianPubMed 35711312
- Pathology oncology research : POR 27:16098092021Clear Cell Papillary Renal Cell Carcinoma Shares Distinct Molecular Characteristics and may be Significantly Associated With Higher Risk of Developing Second Primary Malignancy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXi Tian,Wen-Hao Xu,Jun-Long Wu,Hua-Lei Gan,Hong-Kai Wang,Wei-Jie Gu,Yuan-Yuan Qu,Hai-Liang Zhang,Ding-Wei YePubMed 34512202
- Cell transplantation 30:96368972110344522021Taxifolin Protects Dental Pulp Stem Cells under Hypoxia and Inflammation Conditions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaohui Fu,Yimiao Feng,Bingyi Shao,Yanzhen ZhangPubMed 34292054
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | IHC-Fr | ICC | Flow Cyt | |
|---|---|---|---|---|---|---|
| Human | Expected | Not recommended | Tested | Expected | Not recommended | Not recommended |
| Mouse | Tested | Tested | Tested | Tested | Not recommended | Not recommended |
| Rat | Expected | Tested | Tested | Tested | Not recommended | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/30 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/2000 | Notes - |
Species Rat | Dilution info 1/2000 | Notes - |
Species Human | Dilution info 1/2000 | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 | Notes - |
Species Rat | Dilution info 1/100 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia.
Alternative names
Carbonic anhydrase 9, Carbonate dehydratase IX, Carbonic anhydrase IX, Membrane antigen MN, P54/58N, Renal cell carcinoma-associated antigen G250, pMW1, CA-IX, CAIX, RCC-associated antigen G250, CA9, G250, MN
Recommended products
Rabbit Recombinant Monoclonal Carbonic Anhydrase 9 antibody. Suitable for IP, WB, IHC-P, IHC-Fr and reacts with Mouse, Rat, Human samples. Cited in 8 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR23055-5
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
9 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (ab243660), expandable thumbnail](https://content.abcam.com/products/images/carbonic-anhydrase-9-ca9-antibody-epr23055-5-ab243660--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img58712.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (ab243660)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (ab243660)
Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling Carbonic Anhydrase 9/CA9 with ab243660 at 1/2000 dilution (0.262 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Membranous staining on human stomach (PMID: 15951534) is observed. The section was incubated with ab243660 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
![Immunohistochemistry (Frozen sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (ab243660), expandable thumbnail](https://content.abcam.com/products/images/carbonic-anhydrase-9-ca9-antibody-epr23055-5-ab243660--immunohistochemistry-frozen-sections-img64030.jpg "Immunohistochemistry (Frozen sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (ab243660)")
Immunohistochemistry (Frozen sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (ab243660)
Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen Rat stomach tissue labeling Carbonic Anhydrase 9/CA9 with ab243660 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution. The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution.
![Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (ab243660), expandable thumbnail](https://content.abcam.com/products/images/carbonic-anhydrase-9-ca9-antibody-epr23055-5-ab243660--western-blot-img61287.jpg "Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (ab243660)")
Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (ab243660)
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 15 seconds.
All lanes: Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (ab243660) at 1/1000 dilution
Lane 1: Mouse colon tissue lysate at 20 µg
Lane 2: Mouse brain tissue lysate at 20 µg
Lane 3: Mouse spleen tissue lysate at 20 µg
Lane 4: Rat colon tissue lysate at 20 µg
Lane 5: Rat brain tissue lysate at 20 µg
Lane 6: Rat spleen tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 31 kDa, 49 kDa
Exposure time: 15s
Blocking/diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 15 second
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (ab243660), expandable thumbnail](https://content.abcam.com/products/images/carbonic-anhydrase-9-ca9-antibody-epr23055-5-ab243660--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img117564.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (ab243660)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (ab243660)
Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling Carbonic Anhydrase 9/CA9 with ab243660 at 1/2000 dilution (0.262 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Membranous staining on rat stomach is observed. The section was incubated with ab243660 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (ab243660), expandable thumbnail](https://content.abcam.com/products/images/carbonic-anhydrase-9-ca9-antibody-epr23055-5-ab243660--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img116113.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (ab243660)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (ab243660)
Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling Carbonic Anhydrase 9/CA9 with ab243660 at 1/2000 dilution (0.262 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Membranous staining on mouse stomach is observed. The section was incubated with ab243660 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
![Immunoprecipitation - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (ab243660), expandable thumbnail](https://content.abcam.com/products/images/carbonic-anhydrase-9-ca9-antibody-epr23055-5-ab243660--immunoprecipitation-img3014.jpg "Immunoprecipitation - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (ab243660)")
Immunoprecipitation - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (ab243660)
Carbonic Anhydrase 9/CA9 was immunoprecipitated from 0.35 mg 4T1 (mouse mammary gland carcinoma epithelial cell) whole cell lysate with ab243660 at 1/30 dilution. Western blot was performed on the immunoprecipitate using 243660 at 1/1000 dilution (0.52 μg/ml). VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: 4T1 (mouse mammary gland carcinoma epithelial cell) whole cell lysate 10 μg
Lane 2: ab243660 IP in 4T1 whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab243660 in 4T1 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 48
All lanes: Immunoprecipitation - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (ab243660)
Predicted band size: 49 kDa
![Immunohistochemistry (Frozen sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (ab243660), expandable thumbnail](https://content.abcam.com/products/images/carbonic-anhydrase-9-ca9-antibody-epr23055-5-ab243660--immunohistochemistry-frozen-sections-img125178.jpg "Immunohistochemistry (Frozen sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (ab243660)")
Immunohistochemistry (Frozen sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (ab243660)
Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen Mouse stomach tissue labeling Carbonic Anhydrase 9/CA9 with ab243660 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution. The nuclear counterstain was DAPI (Blue). Positive staining on mouse stomach tissue is observed.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1:1000 (2 μg/ml) dilution.
![Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (ab243660), expandable thumbnail](https://content.abcam.com/products/images/carbonic-anhydrase-9-ca9-antibody-epr23055-5-ab243660--western-blot-img54649.jpg "Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (ab243660)")
Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (ab243660)
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 1 second.
All lanes: Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (ab243660) at 1/1000 dilution
Lane 1: L-929 ( mouse connective tissue fibroblast) whole cell lysate at 20 µg
Lane 2: A20 ( mouse reticulum sarcoma b lymphocyte) whole cell lysate at 20 µg
Lane 3: 4T1 ( mouse mammary gland carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 4: PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Predicted band size: 31 kDa, 49 kDa
Exposure time: 1s
Blocking/diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 1 second
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (ab243660), expandable thumbnail](https://content.abcam.com/products/images/carbonic-anhydrase-9-ca9-antibody-epr23055-5-ab243660--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img418523.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (ab243660)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (ab243660)
Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling Carbonic Anhydrase 9/CA9 with ab243660 at 1/2000 dilution (0.262 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). The immunostaining was performed on a Leica Biosystems BOND® RX instrument counterstained with Hematoxylin. Positive staining on human colon carcinoma. The section was incubated with ab243660 for 10 mins at room temperature.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunoprecipitation - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (ab243660), expandable thumbnail](https://content.abcam.com/products/images/carbonic-anhydrase-9-ca9-antibody-epr23055-5-ab243660--immunoprecipitation-img3014.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (ab243660)")
![Immunohistochemistry (Frozen sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (ab243660), expandable thumbnail](https://content.abcam.com/products/images/carbonic-anhydrase-9-ca9-antibody-epr23055-5-ab243660--immunohistochemistry-frozen-sections-img125178.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (ab243660)")
![Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (ab243660), expandable thumbnail](https://content.abcam.com/products/images/carbonic-anhydrase-9-ca9-antibody-epr23055-5-ab243660--western-blot-img54649.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (ab243660)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (ab243660), expandable thumbnail](https://content.abcam.com/products/images/carbonic-anhydrase-9-ca9-antibody-epr23055-5-ab243660--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img418523.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (ab243660)")
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