Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5]

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (AB243660)

Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling Carbonic Anhydrase 9/CA9 with ab243660 at 1/2000 dilution (0.262 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on human stomach (PMID : 15951534) is observed. The section was incubated with ab243660 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (AB243660)

Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling Carbonic Anhydrase 9/CA9 with ab243660 at 1/2000 dilution (0.262 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The immunostaining was performed on a Leica Biosystems BOND® RX instrument counterstained with Hematoxylin. Positive staining on human colon carcinoma. The section was incubated with ab243660 for 10 mins at room temperature. Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (AB243660)

Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen Rat stomach tissue labeling Carbonic Anhydrase 9/CA9 with ab243660 at 1/100 dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution. The nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (AB243660)

Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling Carbonic Anhydrase 9/CA9 with ab243660 at 1/2000 dilution (0.262 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on mouse stomach is observed. The section was incubated with ab243660 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (AB243660)

Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen Mouse stomach tissue labeling Carbonic Anhydrase 9/CA9 with ab243660 at 1/100 dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution. The nuclear counterstain was DAPI (Blue). Positive staining on mouse stomach tissue is observed.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1 : 1000 (2 μg/ml) dilution.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (AB243660)

Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling Carbonic Anhydrase 9/CA9 with ab243660 at 1/2000 dilution (0.262 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on rat stomach is observed. The section was incubated with ab243660 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

• IP

Lab

Immunoprecipitation - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (AB243660)

Carbonic Anhydrase 9/CA9 was immunoprecipitated from 0.35 mg 4T1 (mouse mammary gland carcinoma epithelial cell) whole cell lysate with ab243660 at 1/30 dilution. Western blot was performed on the immunoprecipitate using 243660 at 1/1000 dilution (0.52 μg/ml). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : 4T1 (mouse mammary gland carcinoma epithelial cell) whole cell lysate 10 μg

Lane 2 : ab243660 IP in 4T1 whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab243660 in 4T1 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 48

All lanes:

Immunoprecipitation - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (ab243660)

Predicted band size: 49 kDa

false

• WB

Lab

Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (AB243660)

Blocking/Diluting buffer and concentration : 5% NFDM/TBST

Exposure time : 1 second.

All lanes:

Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (ab243660) at 1/1000 dilution

Lane 1:

L-929 ( mouse connective tissue fibroblast) whole cell lysate at 20 µg

Lane 2:

A20 ( mouse reticulum sarcoma b lymphocyte) whole cell lysate at 20 µg

Lane 3:

4T1 ( mouse mammary gland carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 31 kDa,49 kDa

false

Exposure time: 1s

• WB

Lab

Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (AB243660)

Blocking/Diluting buffer and concentration : 5% NFDM/TBST

Exposure time : 15 seconds.

All lanes:

Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (ab243660) at 1/1000 dilution

Lane 1:

Mouse colon tissue lysate at 20 µg

Lane 2:

Mouse brain tissue lysate at 20 µg

Lane 3:

Mouse spleen tissue lysate at 20 µg

Lane 4:

Rat colon tissue lysate at 20 µg

Lane 5:

Rat brain tissue lysate at 20 µg

Lane 6:

Rat spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 31 kDa,49 kDa

false

Exposure time: 15s