AB270401

Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] - BSA and Azide free

Name: Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] - BSA and Azide free (ab270401) | Abcam
Brand: Abcam Limited
SKU: AB270401
Price: 905 USD
Availability: InStock
Rating: 4 (1 reviews)

BOND RX™ Validated
RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Carbonic Anhydrase 9 antibody. Carrier free. Suitable for IP, WB, IHC-P, IHC-Fr and reacts with Mouse, Human, Rat samples.

View Alternative Names

G250, MN, CA9, Carbonic anhydrase 9, Carbonate dehydratase IX, Carbonic anhydrase IX, Membrane antigen MN, P54/58N, Renal cell carcinoma-associated antigen G250, pMW1, CA-IX, CAIX, RCC-associated antigen G250

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] - BSA and Azide free (AB270401)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] - BSA and Azide free (AB270401)

Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling Carbonic Anhydrase 9/CA9 with ab243660 at 1/2000 dilution (0.262 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on human stomach (PMID : 15951534) is observed. The section was incubated with ab243660 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] - BSA and Azide free (AB270401)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243660). Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling Carbonic Anhydrase 9/CA9 with ab243660 at 1/2000 dilution (0.262 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The immunostaining was performed on a Leica Biosystems BOND® RX instrument counterstained with Hematoxylin. Positive staining on human colon carcinoma. The section was incubated with ab243660 for 10 mins at room temperature. Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Frozen sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] - BSA and Azide free (AB270401)

IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] - BSA and Azide free (AB270401)

Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen Rat stomach tissue labeling Carbonic Anhydrase 9/CA9 with ab243660 at 1/100 dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution. The nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] - BSA and Azide free (AB270401)

Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling Carbonic Anhydrase 9/CA9 with ab243660 at 1/2000 dilution (0.262 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on rat stomach is observed. The section was incubated with ab243660 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243660).

IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] - BSA and Azide free (AB270401)

Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen Mouse stomach tissue labeling Carbonic Anhydrase 9/CA9 with ab243660 at 1/100 dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution. The nuclear counterstain was DAPI (Blue). Positive staining on mouse stomach tissue is observed.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1 : 1000 (2 μg/ml) dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243660).

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] - BSA and Azide free (AB270401)

Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling Carbonic Anhydrase 9/CA9 with ab243660 at 1/2000 dilution (0.262 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on mouse stomach is observed. The section was incubated with ab243660 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243660).

Lab

Immunoprecipitation - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] - BSA and Azide free (AB270401)

Carbonic Anhydrase 9/CA9 was immunoprecipitated from 0.35 mg 4T1 (mouse mammary gland carcinoma epithelial cell) whole cell lysate with ab243660 at 1/30 dilution. Western blot was performed on the immunoprecipitate using 243660 at 1/1000 dilution (0.52 μg/ml). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : 4T1 (mouse mammary gland carcinoma epithelial cell) whole cell lysate 10 μg

Lane 2 : ab243660 IP in 4T1 whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab243660 in 4T1 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 48

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243660).

All lanes:

Immunoprecipitation - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] (<a href='/en-us/products/primary-antibodies/carbonic-anhydrase-9-ca9-antibody-epr23055-5-ab243660'>ab243660</a>)

Predicted band size: 49 kDa

false

Unconjugated

Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23055-5

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IHC-P, IHC-Fr, IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

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Product details

ab270401 is the carrier-free version of ab243660.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the interconversion between carbon dioxide and water and the dissociated ions of carbonic acid (i.e. bicarbonate and hydrogen ions).

See full target information CA9

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] - BSA and Azide free (AB270401)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] - BSA and Azide free (AB270401)

Immunohistochemistry (Frozen sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] - BSA and Azide free (AB270401)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] - BSA and Azide free (AB270401)

Immunohistochemistry (Frozen sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] - BSA and Azide free (AB270401)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] - BSA and Azide free (AB270401)

Immunoprecipitation - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR23055-5] - BSA and Azide free (AB270401)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Product promise