Rabbit Recombinant Monoclonal Carbonic Anhydrase 9 antibody. Carrier free. Suitable for WB, ICC/IF, IHC-P and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human CA9.
Images
![Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody [HL1410] - BSA and Azide free (AB313848), expandable thumbnail](https://content.abcam.com/products/images/carbonic-anhydrase-9-ca9-antibody-hl1410-bsa-and-azide-free-ab313848--western-blot-img419090.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody [HL1410] - BSA and Azide free (AB313848)")
![Immunocytochemistry/ Immunofluorescence - Anti-Carbonic Anhydrase 9/CA9 antibody [HL1410] - BSA and Azide free (AB313848), expandable thumbnail](https://content.abcam.com/products/images/carbonic-anhydrase-9-ca9-antibody-hl1410-bsa-and-azide-free-ab313848--immunocytochemistry-immunofluorescence-img419088.png/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Carbonic Anhydrase 9/CA9 antibody [HL1410] - BSA and Azide free (AB313848)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [HL1410] - BSA and Azide free (AB313848), expandable thumbnail](https://content.abcam.com/products/images/carbonic-anhydrase-9-ca9-antibody-hl1410-bsa-and-azide-free-ab313848--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img419089.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [HL1410] - BSA and Azide free (AB313848)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human CA9. Database link Q16790
Reactivity data
Select an application| WB | ICC/IF | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Expected |
| Mouse | Expected | Expected | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000.00000 - 1/10000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Catalyzes the interconversion between carbon dioxide and water and the dissociated ions of carbonic acid (i.e. bicarbonate and hydrogen ions).
Additional Targets
Ca9
Alternative names
G250, MN, CA9, Carbonic anhydrase 9, Carbonate dehydratase IX, Carbonic anhydrase IX, Membrane antigen MN, P54/58N, Renal cell carcinoma-associated antigen G250, pMW1, CA-IX, CAIX, RCC-associated antigen G250
Recommended products
Rabbit Recombinant Monoclonal Carbonic Anhydrase 9 antibody. Carrier free. Suitable for WB, ICC/IF, IHC-P and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human CA9.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human CA9. Database link Q16790
- Carrier free
- Yes
- Clone number
- HL1410
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Aliquoting information
- Upon delivery aliquot
Notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody [HL1410] - BSA and Azide free (ab313848), expandable thumbnail](https://content.abcam.com/products/images/carbonic-anhydrase-9-ca9-antibody-hl1410-bsa-and-azide-free-ab313848--western-blot-img419090.jpg "Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody [HL1410] - BSA and Azide free (ab313848)")
Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody [HL1410] - BSA and Azide free (ab313848)
All lanes: Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody [HL1410] - BSA and Azide free (ab313848) at 1/10000 dilution
Lane 1: Whole cell extract from untreated HeLa (Human epithelial cell line from cervix adenocarcinoma) cells at 30 µg
Lane 2: Whole cell extract from HeLa cells treated with 500 uM CoCl2 for 24 hours at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Developed using the ECL technique.
Predicted band size: 49 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Carbonic Anhydrase 9/CA9 antibody [HL1410] - BSA and Azide free (ab313848), expandable thumbnail](https://content.abcam.com/products/images/carbonic-anhydrase-9-ca9-antibody-hl1410-bsa-and-azide-free-ab313848--immunocytochemistry-immunofluorescence-img419088.png "Immunocytochemistry/ Immunofluorescence - Anti-Carbonic Anhydrase 9/CA9 antibody [HL1410] - BSA and Azide free (ab313848)")
Immunocytochemistry/ Immunofluorescence - Anti-Carbonic Anhydrase 9/CA9 antibody [HL1410] - BSA and Azide free (ab313848)
Immunofluorescent analysis of un-treated and CoCl2 treated HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Carbonic Anhydrase 9/CA9 using ab313848 at a 1/500 dilution (green). Blue: Hoechst 33342 staining.
Cells were fixed in ice-cold MeOH for 5 min.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [HL1410] - BSA and Azide free (ab313848), expandable thumbnail](https://content.abcam.com/products/images/carbonic-anhydrase-9-ca9-antibody-hl1410-bsa-and-azide-free-ab313848--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img419089.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [HL1410] - BSA and Azide free (ab313848)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [HL1410] - BSA and Azide free (ab313848)
Immunohistochemical analysis of paraffin-embedded mouse intestine and urinary bladder tissue labeling Carbonic Anhydrase 9/CA9 using Anti-SARS-CoV-2 (COVID-19) nsp10 antibody [HL1381] - BSA and Azide free ab313847 at a 1/100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 mins.
Downloads
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- Visit the general protocols
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