Anti-Carboxymethyl Lysine antibody [CML26]
Be the first to review this product! Submit a review
|
(20 Publications)
- Over 10 publications
- Flow Cyt
Unknown
Flow Cytometry - Anti-Carboxymethyl Lysine antibody [CML26] (AB125145)
Overlay histogram showing HepG2 cells stained with ab125145 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab125145, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Reactivity data
Product details
Anti-Carboxymethyl Lysine antibody [CML26] (ab125145) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA. Trusted by the scientific community
Anti-Carboxymethyl Lysine [CML26] (ab125145) was first used in a scientific publication in 2012 and has been cited over 10 times in peer-reviewed journals.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
Target data
Publications (20)
Recent publications for all applications. Explore the full list and refine your search
Scientific reports 15:35226 PubMed41068344
2025
Applications
Unspecified application
Species
Unspecified reactive species
International journal of nanomedicine 20:11417-11434 PubMed40989892
2025
Applications
Unspecified application
Species
Unspecified reactive species
Arteriosclerosis, thrombosis, and vascular biology 45:424-442 PubMed39817328
2025
Applications
Unspecified application
Species
Unspecified reactive species
International journal of biological sciences 20:880-896 PubMed38250151
2024
Applications
Unspecified application
Species
Unspecified reactive species
Cells 12: PubMed38067186
2023
Applications
Unspecified application
Species
Unspecified reactive species
Cells 12: PubMed37174618
2023
Applications
Unspecified application
Species
Unspecified reactive species
World journal of diabetes 14:222-233 PubMed37035231
2023
Applications
Unspecified application
Species
Unspecified reactive species
Biomolecules 13: PubMed36979358
2023
Applications
Unspecified application
Species
Unspecified reactive species
World journal of diabetes 13:972-985 PubMed36437860
2022
Applications
Unspecified application
Species
Unspecified reactive species
International journal of molecular sciences 23: PubMed35742982
2022
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com