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AB125145

Anti-Carboxymethyl Lysine antibody [CML26]

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(20 Publications)

Anti-Carboxymethyl Lysine antibody [CML26] (ab125145) is a mouse monoclonal antibody detecting Carboxymethyl Lysine in Western Blot, Flow Cytometry, IHC-P, IHC-Fr, ICC/IF, ELISA.

- Over 10 publications
1 Images
Flow Cytometry - Anti-Carboxymethyl Lysine antibody [CML26] (AB125145)
  • Flow Cyt

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Flow Cytometry - Anti-Carboxymethyl Lysine antibody [CML26] (AB125145)

Overlay histogram showing HepG2 cells stained with ab125145 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab125145, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CML26

Isotype

IgG1

Carrier free

No

Applications

Flow Cyt, ICC/IF, IHC-Fr, ELISA, WB, IHC-P

applications

Reactivity data

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Product details

What is this antibody validated in?
Anti-Carboxymethyl Lysine antibody [CML26] (ab125145) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA. Trusted by the scientific community
Anti-Carboxymethyl Lysine [CML26] (ab125145) was first used in a scientific publication in 2012 and has been cited over 10 times in peer-reviewed journals.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Publications (20)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:35226 PubMed41068344

2025

Dual-targeting antioxidant and anti-glycation strategy inhibits melanogenesis through clinical and mechanistic study.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Ye,Jian Mu,Simin Lin,Tiantian Zhang,Fang Ye,Yuying Wang,Lingling Xuan,Changchang Chen,Panpan Yang,Jing Wang,Hu Huang

International journal of nanomedicine 20:11417-11434 PubMed40989892

2025

Iontophoretic Delivery of Hyaluronic Acid Nanogel-Loaded Fermented Rice Extract Mitigates Skin Glycation.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Han,Yunzhi Lin,Miao Guo,Yinshu Wang,Xiuju Dong,Lidan Xiong,Li Li,Hua Wang,Xuefeng Hu,Fan Yang

Arteriosclerosis, thrombosis, and vascular biology 45:424-442 PubMed39817328

2025

ECM Modifications Driven by Age and Metabolic Stress Directly Promote Vascular Smooth Muscle Cell Osteogenic Processes.

Applications

Unspecified application

Species

Unspecified reactive species

Meredith Whitehead,Maria Faleeva,Rafael Oexner,Susan Cox,Lukas Schmidt,Manuel Mayr,Catherine M Shanahan

International journal of biological sciences 20:880-896 PubMed38250151

2024

Nε-(1-Carboxymethyl)-L-lysine/RAGE Signaling Drives Metastasis and Cancer Stemness through ERK/NFκB axis in Osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ting-Yu Chang,Kuo-Cheng Lan,Chia-Hung Wu,Meei-Ling Sheu,Rong-Sen Yang,Shing-Hwa Liu

Cells 12: PubMed38067186

2023

Glycation Interferes with the Expression of Sialyltransferases and Leads to Increased Polysialylation in Glioblastoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Paola Schildhauer,Philipp Selke,Martin S Staege,Anja Harder,Christian Scheller,Christian Strauss,Rüdiger Horstkorte,Maximilian Scheer,Sandra Leisz

Cells 12: PubMed37174618

2023

Glycation Leads to Increased Invasion of Glioblastoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Paola Schildhauer,Philipp Selke,Christian Scheller,Christian Strauss,Rüdiger Horstkorte,Sandra Leisz,Maximilian Scheer

World journal of diabetes 14:222-233 PubMed37035231

2023

N-(carboxymethyl)lysine promotes lipid uptake of macrophage cluster of differentiation 36 and receptor for advanced glycation end products.

Applications

Unspecified application

Species

Unspecified reactive species

Zhong-Qun Wang,Hai-Peng Yao,Zhen Sun

Biomolecules 13: PubMed36979358

2023

Glycation Interferes with the Activity of the Bi-Functional UDP--Acetylglucosamine 2-Epimerase/-Acetyl-mannosamine Kinase (GNE).

Applications

Unspecified application

Species

Unspecified reactive species

Vanessa Hagenhaus,Jacob L Gorenflos López,Rebecca Rosenstengel,Carolin Neu,Christian P R Hackenberger,Arif Celik,Klara Weinert,Mai-Binh Nguyen,Kaya Bork,Rüdiger Horstkorte,Astrid Gesper

World journal of diabetes 13:972-985 PubMed36437860

2022

Dietary N-(carboxymethyl) lysine affects cardiac glucose metabolism and myocardial remodeling in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Zhong-Qun Wang,Zhen Sun

International journal of molecular sciences 23: PubMed35742982

2022

SCA Slows the Decline of Functional Parameters Associated with Senescence in Skin Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Begoña Castro,Naiara de Paz,Salvador González,Azahara Rodríguez-Luna
View all publications

Product promise

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