AB173283

Anti-Carboxypeptidase A antibody [EPR12086]

RabMAb
Recombinant
What is this?

5

(1 Review)

|

(5 Publications)

Rabbit Recombinant Monoclonal CBPA1 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 5 publications.

View Alternative Names

CPA, CPA1, Carboxypeptidase A1

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] (AB173283)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] (AB173283)

ab173283 showing -ve staining in Human normal uterus tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] (AB173283)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] (AB173283)

ab173283 showing -ve staining in Human cervical carcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] (AB173283)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] (AB173283)

ab173283 showing -ve staining in Human normal spleen tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] (AB173283)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] (AB173283)

ab173283 showing +ve staining in Human normal pancreas tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] (AB173283)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] (AB173283)

ab173283 showing -ve staining in Human breast carcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] (AB173283)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] (AB173283)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human pancreas tissue labeling Carboxypeptidase A1 using ab173283 at a 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] (AB173283)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] (AB173283)

ab173283 showing -ve staining in Human normal brain tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-Carboxypeptidase A antibody [EPR12086] (AB173283)

WB

Supplier Data

Western blot - Anti-Carboxypeptidase A antibody [EPR12086] (AB173283)

All lanes:

Western blot - Anti-Carboxypeptidase A antibody [EPR12086] (ab173283) at 1/1000 dilution

Lane 1:

Human pancreas lysate at 10 µg

Lane 2:

mouse pancreas lysate at 10 µg

Lane 3:

rat pancreas lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 47 kDa

true

Carrier free

Anti-Carboxypeptidase A antibody [EPR12086] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12086

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "<p>The use of an HRP/AP polymerized secondary antibody is recommended.</p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Carboxypeptidase that catalyzes the release of a C-terminal amino acid, but has little or no action with -Asp, -Glu, -Arg, -Lys or -Pro (PubMed : 20385563, PubMed : 8806703). Catalyzes the conversion of leukotriene C4 to leukotriene F4 via the hydrolysis of an amide bond (By similarity).

See full target information CPA1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Methods in molecular biology (Clifton, N.J.) 2445:243-253 PubMed34972996

2022

Analysis of Pancreatic Acinar Protein Solubility in Autophagy-Deficient Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew Smith,Carla Salomo-Coll,Morwenna Muir,Simon Wilkinson

Histochemistry and cell biology 155:561-580 PubMed33492488

2021

Protease profile of normal and neoplastic mast cells in the human bone marrow with special emphasis on systemic mastocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Dmitri Atiakshin,Igor Buchwalow,Peter Horny,Markus Tiemann

Proceedings of the National Academy of Sciences of 117:23751-23761 PubMed32917815

2020

Perinatal androgens organize sex differences in mast cells and attenuate anaphylaxis severity into adulthood.

Applications

Unspecified application

Species

Unspecified reactive species

Emily Mackey,Kyan M Thelen,Vedrana Bali,Mahsa Fardisi,Madalyn Trowbridge,Cynthia L Jordan,Adam J Moeser

Translational oncology 11:691-699 PubMed29631213

2018

Label-Free Quantitative Proteomics Unravels Carboxypeptidases as the Novel Biomarker in Pancreatic Ductal Adenocarcinoma.

Applications

WB

Species

Human

Yang Song,Qing Wang,Desheng Wang, Junqiang Li,Jing Yang,Hong Li,Xiang Wang,Xuerong Jin,Ruirui Jing,Jing-Hua Yang,Haichuan Su

Developmental cell 44:217-232.e11 PubMed29290589

2018

CCPG1 Is a Non-canonical Autophagy Cargo Receptor Essential for ER-Phagy and Pancreatic ER Proteostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew D Smith,Margaret E Harley,Alain J Kemp,Jimi Wills,Martin Lee,Mark Arends,Alex von Kriegsheim,Christian Behrends,Simon Wilkinson

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com