Rabbit Recombinant Monoclonal CBPA1 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 5 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] (AB173283), expandable thumbnail](https://content.abcam.com/products/images/carboxypeptidase-a-antibody-epr12086-ab173283--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img114648.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] (AB173283)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] (AB173283), expandable thumbnail](https://content.abcam.com/products/images/carboxypeptidase-a-antibody-epr12086-ab173283--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img77026.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] (AB173283)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] (AB173283), expandable thumbnail](https://content.abcam.com/products/images/carboxypeptidase-a-antibody-epr12086-ab173283--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img113908.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] (AB173283)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] (AB173283), expandable thumbnail](https://content.abcam.com/products/images/carboxypeptidase-a-antibody-epr12086-ab173283--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img9557.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] (AB173283)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] (AB173283), expandable thumbnail](https://content.abcam.com/products/images/carboxypeptidase-a-antibody-epr12086-ab173283--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img88487.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] (AB173283)")
Publications
Filter by
- Methods in molecular biology (Clifton, N.J.) 2445:243-2532022Analysis of Pancreatic Acinar Protein Solubility in Autophagy-Deficient Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMatthew Smith,Carla Salomo-Coll,Morwenna Muir,Simon WilkinsonPubMed 34972996
- Histochemistry and cell biology 155:561-5802021Protease profile of normal and neoplastic mast cells in the human bone marrow with special emphasis on systemic mastocytosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDmitri Atiakshin,Igor Buchwalow,Peter Horny,Markus TiemannPubMed 33492488
- Proceedings of the National Academy of Sciences of 117:23751-237612020Perinatal androgens organize sex differences in mast cells and attenuate anaphylaxis severity into adulthood.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEmily Mackey et. al.PubMed 32917815
- Translational oncology 11:691-6992018Label-Free Quantitative Proteomics Unravels Carboxypeptidases as the Novel Biomarker in Pancreatic Ductal Adenocarcinoma.
- Developmental cell 44:217-232.e112018CCPG1 Is a Non-canonical Autophagy Cargo Receptor Essential for ER-Phagy and Pancreatic ER Proteostasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMatthew D Smith,Margaret E Harley,Alain J Kemp,Jimi Wills,Martin Lee,Mark Arends,Alex von Kriegsheim,Christian Behrends,Simon WilkinsonPubMed 29290589
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Not recommended |
| Mouse | Expected | Not recommended | Tested | Not recommended |
| Rat | Expected | Not recommended | Tested | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/250 - 1/500 | Notes The use of an HRP/AP polymerized secondary antibody is recommended. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/5000 | Notes - |
Species Mouse | Dilution info 1/1000 - 1/5000 | Notes - |
Species Rat | Dilution info 1/1000 - 1/5000 | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Carboxypeptidase that catalyzes the release of a C-terminal amino acid, but has little or no action with -Asp, -Glu, -Arg, -Lys or -Pro (PubMed:20385563, PubMed:8806703). Catalyzes the conversion of leukotriene C4 to leukotriene F4 via the hydrolysis of an amide bond (By similarity).
Alternative names
CPA, CPA1, Carboxypeptidase A1
Recommended products
Rabbit Recombinant Monoclonal CBPA1 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 5 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR12086
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] (ab173283), expandable thumbnail](https://content.abcam.com/products/images/carboxypeptidase-a-antibody-epr12086-ab173283--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img114648.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] (ab173283)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] (ab173283)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue, labeling Carboxypeptidase A1 using ab173283 at a 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] (ab173283), expandable thumbnail](https://content.abcam.com/products/images/carboxypeptidase-a-antibody-epr12086-ab173283--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img77026.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] (ab173283)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] (ab173283)
ab173283 showing +ve staining in Human normal pancreas tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] (ab173283), expandable thumbnail](https://content.abcam.com/products/images/carboxypeptidase-a-antibody-epr12086-ab173283--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img113908.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] (ab173283)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] (ab173283)
ab173283 showing -ve staining in Human breast carcinoma tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] (ab173283), expandable thumbnail](https://content.abcam.com/products/images/carboxypeptidase-a-antibody-epr12086-ab173283--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img9557.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] (ab173283)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] (ab173283)
ab173283 showing -ve staining in Human normal uterus tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] (ab173283), expandable thumbnail](https://content.abcam.com/products/images/carboxypeptidase-a-antibody-epr12086-ab173283--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img88487.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] (ab173283)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] (ab173283)
ab173283 showing -ve staining in Human normal spleen tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] (ab173283), expandable thumbnail](https://content.abcam.com/products/images/carboxypeptidase-a-antibody-epr12086-ab173283--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img95059.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] (ab173283)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] (ab173283)
ab173283 showing -ve staining in Human cervical carcinoma tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] (ab173283), expandable thumbnail](https://content.abcam.com/products/images/carboxypeptidase-a-antibody-epr12086-ab173283--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135317.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] (ab173283)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] (ab173283)
ab173283 showing -ve staining in Human normal brain tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Western blot - Anti-Carboxypeptidase A antibody [EPR12086] (ab173283), expandable thumbnail](https://content.abcam.com/products/images/carboxypeptidase-a-antibody-epr12086-ab173283--western-blot-img62822.jpg "Western blot - Anti-Carboxypeptidase A antibody [EPR12086] (ab173283)")
Western blot - Anti-Carboxypeptidase A antibody [EPR12086] (ab173283)
All lanes: Western blot - Anti-Carboxypeptidase A antibody [EPR12086] (ab173283) at 1/1000 dilution
Lane 1: Human pancreas lysate at 10 µg
Lane 2: mouse pancreas lysate at 10 µg
Lane 3: rat pancreas lysate at 10 µg
SecondaryAll lanes: Goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 47 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] (ab173283), expandable thumbnail](https://content.abcam.com/products/images/carboxypeptidase-a-antibody-epr12086-ab173283--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img95059.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] (ab173283)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] (ab173283), expandable thumbnail](https://content.abcam.com/products/images/carboxypeptidase-a-antibody-epr12086-ab173283--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135317.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] (ab173283)")
![Western blot - Anti-Carboxypeptidase A antibody [EPR12086] (ab173283), expandable thumbnail](https://content.abcam.com/products/images/carboxypeptidase-a-antibody-epr12086-ab173283--western-blot-img62822.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Carboxypeptidase A antibody [EPR12086] (ab173283)")
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