Rabbit Recombinant Monoclonal CBPA1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] - BSA and Azide free (AB249747), expandable thumbnail](https://content.abcam.com/products/images/carboxypeptidase-a-antibody-epr12086-bsa-and-azide-free-ab249747--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img114648.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] - BSA and Azide free (AB249747)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] - BSA and Azide free (AB249747), expandable thumbnail](https://content.abcam.com/products/images/carboxypeptidase-a-antibody-epr12086-bsa-and-azide-free-ab249747--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img77026.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] - BSA and Azide free (AB249747)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] - BSA and Azide free (AB249747), expandable thumbnail](https://content.abcam.com/products/images/carboxypeptidase-a-antibody-epr12086-bsa-and-azide-free-ab249747--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img113908.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] - BSA and Azide free (AB249747)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] - BSA and Azide free (AB249747), expandable thumbnail](https://content.abcam.com/products/images/carboxypeptidase-a-antibody-epr12086-bsa-and-azide-free-ab249747--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img9557.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] - BSA and Azide free (AB249747)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] - BSA and Azide free (AB249747), expandable thumbnail](https://content.abcam.com/products/images/carboxypeptidase-a-antibody-epr12086-bsa-and-azide-free-ab249747--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img88487.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] - BSA and Azide free (AB249747)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Not recommended |
| Mouse | Expected | Not recommended | Tested | Not recommended |
| Rat | Expected | Not recommended | Tested | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Use of a HRP/AP polymerized secondary antibody is recommended. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Target data
Function
Carboxypeptidase that catalyzes the release of a C-terminal amino acid, but has little or no action with -Asp, -Glu, -Arg, -Lys or -Pro (PubMed:20385563, PubMed:8806703). Catalyzes the conversion of leukotriene C4 to leukotriene F4 via the hydrolysis of an amide bond (By similarity).
Alternative names
CPA, CPA1, Carboxypeptidase A1
Recommended products
Rabbit Recombinant Monoclonal CBPA1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR12086
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab249747 is the carrier-free version of Anti-Carboxypeptidase A antibody [EPR12086] ab173283.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] - BSA and Azide free (ab249747), expandable thumbnail](https://content.abcam.com/products/images/carboxypeptidase-a-antibody-epr12086-bsa-and-azide-free-ab249747--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img114648.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] - BSA and Azide free (ab249747)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] - BSA and Azide free (ab249747)
This data was developed using Anti-Carboxypeptidase A antibody [EPR12086] ab173283, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue, labeling Carboxypeptidase A1 using Anti-Carboxypeptidase A antibody [EPR12086] ab173283 at a 1/250 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] - BSA and Azide free (ab249747), expandable thumbnail](https://content.abcam.com/products/images/carboxypeptidase-a-antibody-epr12086-bsa-and-azide-free-ab249747--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img77026.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] - BSA and Azide free (ab249747)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] - BSA and Azide free (ab249747)
This data was developed using Anti-Carboxypeptidase A antibody [EPR12086] ab173283, the same antibody clone in a different buffer formulation.Anti-Carboxypeptidase A antibody [EPR12086] ab173283 showing +ve staining in Human normal pancreas tissue. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] - BSA and Azide free (ab249747), expandable thumbnail](https://content.abcam.com/products/images/carboxypeptidase-a-antibody-epr12086-bsa-and-azide-free-ab249747--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img113908.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] - BSA and Azide free (ab249747)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] - BSA and Azide free (ab249747)
This data was developed using Anti-Carboxypeptidase A antibody [EPR12086] ab173283, the same antibody clone in a different buffer formulation.Anti-Carboxypeptidase A antibody [EPR12086] ab173283 showing -ve staining in Human breast carcinoma tissue. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] - BSA and Azide free (ab249747), expandable thumbnail](https://content.abcam.com/products/images/carboxypeptidase-a-antibody-epr12086-bsa-and-azide-free-ab249747--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img9557.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] - BSA and Azide free (ab249747)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] - BSA and Azide free (ab249747)
This data was developed using Anti-Carboxypeptidase A antibody [EPR12086] ab173283, the same antibody clone in a different buffer formulation.Anti-Carboxypeptidase A antibody [EPR12086] ab173283 showing -ve staining in Human normal uterus tissue. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] - BSA and Azide free (ab249747), expandable thumbnail](https://content.abcam.com/products/images/carboxypeptidase-a-antibody-epr12086-bsa-and-azide-free-ab249747--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img88487.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] - BSA and Azide free (ab249747)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] - BSA and Azide free (ab249747)
This data was developed using Anti-Carboxypeptidase A antibody [EPR12086] ab173283, the same antibody clone in a different buffer formulation.Anti-Carboxypeptidase A antibody [EPR12086] ab173283 showing -ve staining in Human normal spleen tissue. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] - BSA and Azide free (ab249747), expandable thumbnail](https://content.abcam.com/products/images/carboxypeptidase-a-antibody-epr12086-bsa-and-azide-free-ab249747--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img95059.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] - BSA and Azide free (ab249747)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] - BSA and Azide free (ab249747)
This data was developed using Anti-Carboxypeptidase A antibody [EPR12086] ab173283, the same antibody clone in a different buffer formulation.Anti-Carboxypeptidase A antibody [EPR12086] ab173283 showing -ve staining in Human cervical carcinoma tissue. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] - BSA and Azide free (ab249747), expandable thumbnail](https://content.abcam.com/products/images/carboxypeptidase-a-antibody-epr12086-bsa-and-azide-free-ab249747--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135317.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] - BSA and Azide free (ab249747)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] - BSA and Azide free (ab249747)
This data was developed using Anti-Carboxypeptidase A antibody [EPR12086] ab173283, the same antibody clone in a different buffer formulation.Anti-Carboxypeptidase A antibody [EPR12086] ab173283 showing -ve staining in Human normal brain tissue. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Western blot - Anti-Carboxypeptidase A antibody [EPR12086] - BSA and Azide free (ab249747), expandable thumbnail](https://content.abcam.com/products/images/carboxypeptidase-a-antibody-epr12086-bsa-and-azide-free-ab249747--western-blot-img62822.jpg "Western blot - Anti-Carboxypeptidase A antibody [EPR12086] - BSA and Azide free (ab249747)")
Western blot - Anti-Carboxypeptidase A antibody [EPR12086] - BSA and Azide free (ab249747)
This data was developed using Anti-Carboxypeptidase A antibody [EPR12086] ab173283, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Carboxypeptidase A antibody [EPR12086] (Anti-Carboxypeptidase A antibody [EPR12086] ab173283) at 1/1000 dilution
Lane 1: Human pancreas lysate at 10 µg
Lane 2: mouse pancreas lysate at 10 µg
Lane 3: rat pancreas lysate at 10 µg
SecondaryAll lanes: Goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 47 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] - BSA and Azide free (ab249747), expandable thumbnail](https://content.abcam.com/products/images/carboxypeptidase-a-antibody-epr12086-bsa-and-azide-free-ab249747--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img95059.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] - BSA and Azide free (ab249747)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] - BSA and Azide free (ab249747), expandable thumbnail](https://content.abcam.com/products/images/carboxypeptidase-a-antibody-epr12086-bsa-and-azide-free-ab249747--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135317.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] - BSA and Azide free (ab249747)")
![Western blot - Anti-Carboxypeptidase A antibody [EPR12086] - BSA and Azide free (ab249747), expandable thumbnail](https://content.abcam.com/products/images/carboxypeptidase-a-antibody-epr12086-bsa-and-azide-free-ab249747--western-blot-img62822.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Carboxypeptidase A antibody [EPR12086] - BSA and Azide free (ab249747)")
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