Anti-Carboxypeptidase A antibody [EPR24384-69] - BSA and Azide free (ab278047)

Rabbit Recombinant Monoclonal CBPA1 antibody. Carrier free. Suitable for mIHC, WB, IHC-Fr, IHC-P and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-Carboxypeptidase A antibody [EPR24384-69] - BSA and Azide free (AB278047), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	mIHC	ICC/IF	IP	Flow Cyt	WB	IHC-Fr	IHC-P
Human	Tested	Not recommended	Not recommended	Not recommended	Tested	Expected	Tested
Mouse	Expected	Not recommended	Not recommended	Not recommended	Tested	Expected	Expected
Rat	Expected	Not recommended	Not recommended	Not recommended	Tested	Tested	Expected

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes -
Species Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Rat	Dilution info -	Notes Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Expected
Expected

Species	Dilution info	Notes
Species Human, Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

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2 products for Alternative Version

Target data

See full target information CPA1

Function

Carboxypeptidase that catalyzes the release of a C-terminal amino acid, but has little or no action with -Asp, -Glu, -Arg, -Lys or -Pro (PubMed:20385563, PubMed:8806703). Catalyzes the conversion of leukotriene C4 to leukotriene F4 via the hydrolysis of an amide bond (By similarity).

Alternative names

CPA, CPA1, Carboxypeptidase A1

Recommended products

Rabbit Recombinant Monoclonal CBPA1 antibody. Carrier free. Suitable for mIHC, WB, IHC-Fr, IHC-P and reacts with Human, Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR24384-69
Purification technique: Affinity purification Protein A
Specificity: This antibody is specific to Carboxypeptidase A1 and has no cross reactivity with other family members tested.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

Anti-Carboxypeptidase A antibody [EPR24384-69] ab278044 is the carrier-free version of ab278047.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

12 product images

Western blot - Anti-Carboxypeptidase A antibody [EPR24384-69] - BSA and Azide free (ab278047)
This data was developed using Anti-Carboxypeptidase A antibody [EPR24384-69] ab278044, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 21098023).
Activation of pro-carboxypeptidase A is initiated by proteolytic cleavage at the C-terminal end of the propeptide by trypsin and chymotrypsin (PMID: 8436102, 21098023).
Exposure time: 3 seconds.
All lanes: Western blot - Anti-Carboxypeptidase A antibody [EPR24384-69] (Anti-Carboxypeptidase A antibody [EPR24384-69] ab278044) at 1/1000 dilution
Lane 1: Human pancreas tissue lysate at 20 µg
Lane 2: Mouse pancreas tissue lysate at 20 µg
Lane 3: Rat pancreas tissue lysate at 20 µg
Secondary
All lanes: VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution
Predicted band size: 47 kDa
Observed band size: 33 kDa, 43 kDa
Immunohistochemistry (Frozen sections) - Anti-Carboxypeptidase A antibody [EPR24384-69] - BSA and Azide free (ab278047)
This data was developed using Anti-Carboxypeptidase A antibody [EPR24384-69] ab278044, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse pancreas tissue labeling Carboxypeptidase A with Anti-Carboxypeptidase A antibody [EPR24384-69] ab278044 at 1/100 (5.39 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (Green). Positive staining on mouse pancreas is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunohistochemistry (Frozen sections) - Anti-Carboxypeptidase A antibody [EPR24384-69] - BSA and Azide free (ab278047)
This data was developed using Anti-Carboxypeptidase A antibody [EPR24384-69] ab278044, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat pancreas tissue labeling Carboxypeptidase A with Anti-Carboxypeptidase A antibody [EPR24384-69] ab278044 at 1/500 (1.078 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^®488) at 1/1000 dilution (Green). Positive staining on rat pancreas is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488)at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR24384-69] - BSA and Azide free (ab278047)
This data was developed using Anti-Carboxypeptidase A antibody [EPR24384-69] ab278044, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Carboxypeptidase A with Anti-Carboxypeptidase A antibody [EPR24384-69] ab278044 at 1/4000 (0.135 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection). Cytoplasmic staining on acinar cells of human pancreas (PMID: 23006325). The section was incubated with Anti-Carboxypeptidase A antibody [EPR24384-69] ab278044 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR24384-69] - BSA and Azide free (ab278047)
This data was developed using Anti-Carboxypeptidase A antibody [EPR24384-69] ab278044, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse pancreas tissue labeling Carboxypeptidase A with Anti-Carboxypeptidase A antibody [EPR24384-69] ab278044 at 1/4000 (0.135 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection). Cytoplasmic staining on acinar cells of mouse pancreas. The section was incubated with Anti-Carboxypeptidase A antibody [EPR24384-69] ab278044 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR24384-69] - BSA and Azide free (ab278047)
This data was developed using Anti-Carboxypeptidase A antibody [EPR24384-69] ab278044, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat pancreas tissue labeling Carboxypeptidase A with Anti-Carboxypeptidase A antibody [EPR24384-69] ab278044 at 1/4000 (0.135 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection). Cytoplasmic staining on acinar cells of rat pancreas. The section was incubated with Anti-Carboxypeptidase A antibody [EPR24384-69] ab278044 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^®RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR24384-69] - BSA and Azide free (ab278047)
This data was developed using Anti-Carboxypeptidase A antibody [EPR24384-69] ab278044, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling Carboxypeptidase A with Anti-Carboxypeptidase A antibody [EPR24384-69] ab278044 at 1/4000 (0.135 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection). Negative control: No staining on human spleen. The section was incubated with Anti-Carboxypeptidase A antibody [EPR24384-69] ab278044 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Multiplex immunohistochemistry - Anti-Carboxypeptidase A antibody [EPR24384-69] - BSA and Azide free (ab278047)
Fluorescence multiplex immunohistochemical analysis of the human pancreas (Formalin/PFA-fixed paraffin-embedded sections).

Panel A: merged staining of anti-Carboxypeptidase A (Anti-Carboxypeptidase A antibody [EPR24384-69] ab278044, gray; Opal™690), anti-GIP (Anti-GIP antibody [EPR20410] - BSA and Azide free ab271989, green; Opal™520) and anti-Somatostatin 28 (Anti-Somatostatin 28 antibody [EPR3359(2)] ab111912, red; Opal™570) on human pancreas. Panel B: anti-Carboxypeptidase A stained on acinar cells. Panel C: anti-GIP stained on alpha cells. Panel D: anti-Somatostatin 28 stained on delta cells. Opal Polymer HRP Ms + Rb was used as a secondary antibody.

The section was incubated in three rounds of staining: in the order of Anti-Carboxypeptidase A antibody [EPR24384-69] ab278044 at 1/4000 dilution (0.135 μg/ml), Anti-GIP antibody [EPR20410] - BSA and Azide free ab271989 at 1/4000 dilution (0.27 μg/ml) and Anti-Somatostatin 28 antibody [EPR3359(2)] ab111912 at 1/9000 dilution (0.068 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain.

This data was developed using Anti-Carboxypeptidase A antibody [EPR24384-69] ab278044, the same antibody clone in a different buffer formulation.
Multiplex immunohistochemistry - Anti-Carboxypeptidase A antibody [EPR24384-69] - BSA and Azide free (ab278047)
Fluorescence multiplex immunohistochemical analysis of the human pancreas (Formalin/PFA-fixed paraffin-embedded sections).

Panel A: merged staining of anti-Carboxypeptidase A (Anti-Carboxypeptidase A antibody [EPR24384-69] ab278044, magenta; Opal™690), anti-Cytokeratin 19 (Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free ab195872, green; Opal™520) and anti-Insulin (Anti-Insulin antibody [EPR17359] ab181547, red; Opal™570) on human pancreas. Panel B: anti-Carboxypeptidase A stained on acinar cells. Panel C: anti-Cytokeratin 19 stained on centroacinar cells and ducts. Panel D: anti-Insulin stained on beta cells. Opal Polymer HRP Ms + Rb was used as a secondary antibody.

The section was incubated in three rounds of staining: in the order of Anti-Carboxypeptidase A antibody [EPR24384-69] ab278044 at 1/4000 dilution (0.135 μg/ml), Anti-Cytokeratin 19 antibody [EP1580Y] - BSA and Azide free ab195872 at 1/8000 dilution (0.127 μg/ml), and Anti-Insulin antibody [EPR17359] ab181547 at 1/20000 dilution (0.053 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain.

This data was developed using Anti-Carboxypeptidase A antibody [EPR24384-69] ab278044, the same antibody clone in a different buffer formulation.
Western blot - Anti-Carboxypeptidase A antibody [EPR24384-69] - BSA and Azide free (ab278047)
This data was developed using Anti-Carboxypeptidase A antibody [EPR24384-69] ab278044, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer: 5% NFDM /TBST

This antibody is specific to Carboxypeptidase A1 and has no cross reactivity with other family members tested

Exposure time: 3.25 seconds
All lanes: Western blot - Anti-Carboxypeptidase A antibody [EPR24384-69] (Anti-Carboxypeptidase A antibody [EPR24384-69] ab278044) at 1/1000 dilution
Lane 1: Human Carboxypeptidase A1 recombinant protein (aa17-419) at 20 ng
Lane 2: His tagged Human Carboxypeptidase A2 recombinant protein (aa20-419) at 20 ng
Lane 3: His tagged Human Carboxypeptidase A4 recombinant protein (aa18-421) at 100 ng
Lane 4: His tagged Human Carboxypeptidase A5 recombinant protein (aa36-416) at 200 ng
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa
Exposure time: 3.25s
Immunohistochemistry (Frozen sections) - Anti-Carboxypeptidase A antibody [EPR24384-69] - BSA and Azide free (ab278047)
Negative tissue image: IHC image of Carboxypeptidase A staining in a section of frozen normal human spleen performed on a Leica Biosystems BOND® RX instrument. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with Anti-Carboxypeptidase A antibody [EPR24384-69] ab278044, 0.05 ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
This data was developed using Anti-Carboxypeptidase A antibody [EPR24384-69] ab278044, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Frozen sections) - Anti-Carboxypeptidase A antibody [EPR24384-69] - BSA and Azide free (ab278047)
IHC image of Carboxypeptidase A staining in a section of frozen normal human pancreas* performed on a Leica Biosystems BOND® RX instrument. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with Anti-Carboxypeptidase A antibody [EPR24384-69] ab278044, 0.05 ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
This data was developed using Anti-Carboxypeptidase A antibody [EPR24384-69] ab278044, the same antibody clone in a different buffer formulation.