Rabbit Recombinant Monoclonal CBPA1 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Rat, Human, Mouse samples. Cited in 2 publications.
Images
![Western blot - Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] (AB181146), expandable thumbnail](https://content.abcam.com/products/images/carboxypeptidase-a1a2b-antibody-epr12087b-ab181146--western-blot-img141961.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] (AB181146)")
![Immunocytochemistry/ Immunofluorescence - Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] (AB181146), expandable thumbnail](https://content.abcam.com/products/images/carboxypeptidase-a1a2b-antibody-epr12087b-ab181146--immunocytochemistry-immunofluorescence-img42109.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] (AB181146)")
![Western blot - Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] (AB181146), expandable thumbnail](https://content.abcam.com/products/images/carboxypeptidase-a1a2b-antibody-epr12087b-ab181146--western-blot-img124141.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] (AB181146)")
Publications
Filter by
- Histochemistry and cell biology 159:353-3612023Chloroacetate esterase reaction combined with immunohistochemical characterization of the specific tissue microenvironment of mast cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDmitri Atiakshin,Andrey Kostin,Igor Buchwalow,Denis Morozow,Vera Samoilova,Markus TiemannPubMed 36598563
- Histochemistry and cell biology 155:561-5802021Protease profile of normal and neoplastic mast cells in the human bone marrow with special emphasis on systemic mastocytosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDmitri Atiakshin,Igor Buchwalow,Peter Horny,Markus TiemannPubMed 33492488
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected |
| Rat | Tested | Expected | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/1000 - 1/5000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/1000 - 1/5000 | Notes - |
Species Mouse | Dilution info 1/1000 - 1/5000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/100 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/250 - 1/500 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Carboxypeptidase that catalyzes the release of a C-terminal amino acid, but has little or no action with -Asp, -Glu, -Arg, -Lys or -Pro (PubMed:8806703). Catalyzes the conversion of leukotriene C4 to leukotriene F4 via the hydrolysis of an amide bond (By similarity).
Targets
CPA2, Carboxypeptidase B
Alternative names
Carboxypeptidase A1, CPA1, CPA
Recommended products
Rabbit Recombinant Monoclonal CBPA1 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Rat, Human, Mouse samples. Cited in 2 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR12087(B)
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] (ab181146), expandable thumbnail](https://content.abcam.com/products/images/carboxypeptidase-a1a2b-antibody-epr12087b-ab181146--western-blot-img141961.jpg "Western blot - Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] (ab181146)")
Western blot - Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] (ab181146)
All lanes: Western blot - Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] (ab181146) at 1/5000 dilution
All lanes: Human pancreas tissue lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 47 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] (ab181146), expandable thumbnail](https://content.abcam.com/products/images/carboxypeptidase-a1a2b-antibody-epr12087b-ab181146--immunocytochemistry-immunofluorescence-img42109.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] (ab181146)")
Immunocytochemistry/ Immunofluorescence - Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] (ab181146)
Immunofluorescent analysis of BxPC3 cells (paraformaldehyde-fixed, 4%) labeling Carboxypeptidase A with ab181146 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).
![Western blot - Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] (ab181146), expandable thumbnail](https://content.abcam.com/products/images/carboxypeptidase-a1a2b-antibody-epr12087b-ab181146--western-blot-img124141.jpg "Western blot - Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] (ab181146)")
Western blot - Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] (ab181146)
All lanes: Western blot - Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] (ab181146) at 1/1000 dilution
Lane 1: Rat pancreas lysate at 10 µg
Lane 2: Mouse pancreas lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 47 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] (ab181146), expandable thumbnail](https://content.abcam.com/products/images/carboxypeptidase-a1a2b-antibody-epr12087b-ab181146--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136813.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] (ab181146)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] (ab181146)
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Carboxypeptidase A with ab181146 at 1/500 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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