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AB181146

Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)]

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(4 Publications)

Rabbit Recombinant Monoclonal CBPA1 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Rat, Human, Mouse samples. Cited in 4 publications.

View Alternative Names

CPA, CPA1, Carboxypeptidase A1, Carboxypeptidase A2, CPA2, CPB, PCPB, CPB1, Carboxypeptidase B, Pancreas-specific protein, PASP

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] (AB181146)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] (AB181146)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Carboxypeptidase A with ab181146 at 1/500 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] (AB181146)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] (AB181146)

Immunofluorescent analysis of BxPC3 cells (paraformaldehyde-fixed 4%) labeling Carboxypeptidase A with ab181146 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).

Western blot - Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] (AB181146)
  • WB

Supplier Data

Western blot - Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] (AB181146)

All lanes:

Western blot - Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] (ab181146) at 1/5000 dilution

All lanes:

Human pancreas tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 47 kDa

false

Western blot - Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] (AB181146)
  • WB

Supplier Data

Western blot - Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] (AB181146)

All lanes:

Western blot - Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] (ab181146) at 1/1000 dilution

Lane 1:

Rat pancreas lysate at 10 µg

Lane 2:

Mouse pancreas lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 47 kDa

false

  • Carrier free

    Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12087(B)

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Primary Antibodies

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AB24170

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  • 4
  • 100
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Carboxypeptidase that catalyzes the release of a C-terminal amino acid, but has little or no action with -Asp, -Glu, -Arg, -Lys or -Pro (PubMed : 20385563, PubMed : 8806703). Catalyzes the conversion of leukotriene C4 to leukotriene F4 via the hydrolysis of an amide bond (By similarity).
See full target information CPA1

Additional targets

CPA2,CPB1
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Histochemistry and cell biology 159:353-361 PubMed36598563

2023

Chloroacetate esterase reaction combined with immunohistochemical characterization of the specific tissue microenvironment of mast cells.

Applications

Unspecified application

Species

Unspecified reactive species

Dmitri Atiakshin,Andrey Kostin,Igor Buchwalow,Denis Morozow,Vera Samoilova,Markus Tiemann

International journal of molecular sciences 23: PubMed36012196

2022

Protease Profile of Tumor-Associated Mast Cells in Melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Dmitri Atiakshin,Andrey Kostin,Igor Buchwalow,Vera Samoilova,Markus Tiemann

Histochemistry and cell biology 155:561-580 PubMed33492488

2021

Protease profile of normal and neoplastic mast cells in the human bone marrow with special emphasis on systemic mastocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Dmitri Atiakshin,Igor Buchwalow,Peter Horny,Markus Tiemann

The Journal of biological chemistry 295:7653-7668 PubMed32321761

2020

The receptor tyrosine kinase EPHB6 regulates catecholamine exocytosis in adrenal gland chromaffin cells.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Shi,Bei Ye,Marion Rame,Yujia Wang,Dominique Cioca,Sophie Reibel,Junzheng Peng,Shijie Qi,Nicolas Vitale,Hongyu Luo,Jiangping Wu
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com