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Rabbit Recombinant Monoclonal CBPA1 antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P and reacts with Human, Rat, Mouse samples.

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Images

Western blot - Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] - BSA and Azide free (AB250379), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] - BSA and Azide free (AB250379), expandable thumbnail
  • Western blot - Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] - BSA and Azide free (AB250379), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] - BSA and Azide free (AB250379), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWBIHC-P
Human
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Rat
Expected
Tested
Expected

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Rat, Human, Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Carboxypeptidase that catalyzes the release of a C-terminal amino acid, but has little or no action with -Asp, -Glu, -Arg, -Lys or -Pro (PubMed:20385563, PubMed:8806703). Catalyzes the conversion of leukotriene C4 to leukotriene F4 via the hydrolysis of an amide bond (By similarity).

Additional Targets

CPA2, CPB1

Alternative names

CPA, CPA1, Carboxypeptidase A1

Recommended products

Rabbit Recombinant Monoclonal CBPA1 antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P and reacts with Human, Rat, Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR12087(B)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab250379 is the carrier-free version of Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] ab181146.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] - BSA and Azide free (ab250379), expandable thumbnail

    Western blot - Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] - BSA and Azide free (ab250379)

    This data was developed using Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] ab181146, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] (Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] ab181146) at 1/5000 dilution

    All lanes: Human pancreas tissue lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 47 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] - BSA and Azide free (ab250379), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] - BSA and Azide free (ab250379)

    This data was developed using Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] ab181146, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of BxPC3 cells (paraformaldehyde-fixed, 4%) labeling Carboxypeptidase A with Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] ab181146 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).

  • Western blot - Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] - BSA and Azide free (ab250379), expandable thumbnail

    Western blot - Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] - BSA and Azide free (ab250379)

    This data was developed using Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] ab181146, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] (Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] ab181146) at 1/1000 dilution

    Lane 1: Rat pancreas lysate at 10 µg

    Lane 2: Mouse pancreas lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 47 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] - BSA and Azide free (ab250379), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] - BSA and Azide free (ab250379)

    This data was developed using Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] ab181146, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Carboxypeptidase A with Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] ab181146 at 1/500 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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