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AB250801

Anti-Carboxypeptidase D/CPD antibody [EPR14811] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Carboxypeptidase D/CPD antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Carboxypeptidase D, Metallocarboxypeptidase D, gp180, CPD

2 Images
Western blot - Anti-Carboxypeptidase D/CPD antibody [EPR14811] - BSA and Azide free (AB250801)
  • WB

Supplier Data

Western blot - Anti-Carboxypeptidase D/CPD antibody [EPR14811] - BSA and Azide free (AB250801)

This data was developed using ab184967, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Carboxypeptidase D/CPD antibody [EPR14811] - C-terminal (<a href='/en-us/products/primary-antibodies/carboxypeptidase-d-cpd-antibody-epr14811-c-terminal-ab184967'>ab184967</a>) at 1/5000 dilution

All lanes:

A431 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 153 kDa

false

Western blot - Anti-Carboxypeptidase D/CPD antibody [EPR14811] - BSA and Azide free (AB250801)
  • WB

Supplier Data

Western blot - Anti-Carboxypeptidase D/CPD antibody [EPR14811] - BSA and Azide free (AB250801)

This data was developed using ab184967, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Carboxypeptidase D/CPD antibody [EPR14811] - C-terminal (<a href='/en-us/products/primary-antibodies/carboxypeptidase-d-cpd-antibody-epr14811-c-terminal-ab184967'>ab184967</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 153 kDa

false

  • Unconjugated

    Anti-Carboxypeptidase D/CPD antibody [EPR14811] - C-terminal

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14811

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

ab250801 is the carrier-free version of ab184967.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Functions in the processing of proteins and peptides in the secretory pathway. Mechanistically, cleaves exclusively C-terminal basic residues. By removing terminal residues, can modulate the activity, stability, and receptor-binding properties of bioactive peptides.. (Microbial infection) Acts as an alternative entry receptor for adeno-associated virus (AAV).
See full target information Carboxypeptidase D
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The American journal of pathology 187:1686-1699 PubMed28618255

2017

Osteocyte Regulation of Receptor Activator of NF-κB Ligand/Osteoprotegerin in a Sheep Model of Osteoporosis.

Applications

Unspecified application

Species

Unspecified reactive species

Thaqif El Khassawna,Felix Merboth,Deeksha Malhan,Wolfgang Böcker,Diaa E S Daghma,Sabine Stoetzel,Stefanie Kern,Fathi Hassan,Dirk Rosenbaum,Judith Langenstein,Natali Bauer,Anja Schlagenhauf,Angela Rösen-Wolff,Felix Schulze,Markus Rupp,Dirk Hose,Anja Secklinger,Anita Ignatius,Hans-Joachim Wilke,Katrin S Lips,Christian Heiss
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com