Mouse Monoclonal Carboxypeptidase Y antibody. Suitable for WB and reacts with Saccharomyces cerevisiae samples. Cited in 7 publications.
Images
![Western blot - Anti-Carboxypeptidase Y antibody [10A5B5] (AB113685), expandable thumbnail](https://content.abcam.com/products/images/carboxypeptidase-y-antibody-10a5b5-ab113685--western-blot-img124013.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Carboxypeptidase Y antibody [10A5B5] (AB113685)")
Publications
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- Nature communications 11:15062020The cryo-EM structure of the SNX-BAR Mvp1 tetramer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDapeng Sun et. al.PubMed 32198400
- Scientific reports 9:107472019Ent3 and GGA adaptors facilitate diverse anterograde and retrograde trafficking events to and from the prevacuolar endosome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFrancisco Yanguas et. al.PubMed 31341193
- Nature 540:134-1382016The SND proteins constitute an alternative targeting route to the endoplasmic reticulum.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNaama Aviram,Tslil Ast,Elizabeth A Costa,Eric C Arakel,Silvia G Chuartzman,Calvin H Jan,Sarah Haßdenteufel,Johanna Dudek,Martin Jung,Stefan Schorr,Richard Zimmermann,Blanche Schwappach,Jonathan S Weissman,Maya SchuldinerPubMed 27905431
- Journal of cell science 127:3017-232014A cytosolic degradation pathway, prERAD, monitors pre-inserted secretory pathway proteins.
- Molecular and cellular biology 33:526-422012Saccharomyces cerevisiae Env7 is a novel serine/threonine kinase 16-related protein kinase and negatively regulates organelle fusion at the lysosomal vacuole.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSurya P Manandhar,Florante Ricarte,Stephanie M Cocca,Editte GharakhanianPubMed 23166297
- The Journal of biological chemistry 287:35709-212012A role for iron-sulfur clusters in the regulation of transcription factor Yap5-dependent high iron transcriptional responses in yeast.
- PloS one 7:e340242012Accumulation of an antidepressant in vesiculogenic membranes of yeast cells triggers autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingqiu Chen et. al.PubMed 22529904
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | |
|---|---|
| Saccharomyces cerevisiae | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Saccharomyces cerevisiae | Dilution info 0.5 µg/mL | Notes - |
Associated Products
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Target data
Function
Vacuolar serine-type carboxypeptidase involved in degradation of small peptides (PubMed:8679540). Digests preferentially peptides containing an aliphatic or hydrophobic residue in P1' position, as well as methionine, leucine or phenylalanine in P1 position of ester substrate (PubMed:8679540). Also plays a role in breakdown of the autophagic body and the autophagosome-dependent protein synthesis (PubMed:29514932). Plays a key role in phytochelatin (PC) synthesis from glutathione (GSH) by cleaving the Gly from GSH and form the PC-peptides of the structure (gamma-Glu-Cys)2-Gly (PubMed:17408619). Also involved in resistance to xenobiotics via the degradation of glutathione-S-conjugates (PubMed:19897216).
Alternative names
YMR297W, PRC1, Carboxypeptidase Y, CPD-Y, cpY, Carboxypeptidase YSCY
Recommended products
Mouse Monoclonal Carboxypeptidase Y antibody. Suitable for WB and reacts with Saccharomyces cerevisiae samples. Cited in 7 publications.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- 10A5B5
- Purification technique
- Proprietary technique
- Light chain type
- kappa
- Concentration
- Purification notes
Near homogeneity (Purity >95% by SDS-PAGE). The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by chemical fractionation.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
![Western blot - Anti-Carboxypeptidase Y antibody [10A5B5] (ab113685), expandable thumbnail](https://content.abcam.com/products/images/carboxypeptidase-y-antibody-10a5b5-ab113685--western-blot-img124013.jpg "Western blot - Anti-Carboxypeptidase Y antibody [10A5B5] (ab113685)")
Western blot - Anti-Carboxypeptidase Y antibody [10A5B5] (ab113685)
All lanes: Western blot - Anti-Carboxypeptidase Y antibody [10A5B5] (ab113685)
Lane 1: Yeast whole cell lysate at 15 µg
Lane 2: Yeast membrane preparation at 15 µg
Developed using the ECL technique.
Performed under reducing conditions.
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