AB113685

Anti-Carboxypeptidase Y antibody [10A5B5]

Name: Anti-Carboxypeptidase Y antibody [10A5B5] (ab113685) | Abcam
Brand: Abcam Limited
SKU: AB113685
Price: 440 USD
Availability: InStock

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(7 Publications)

Mouse Monoclonal Carboxypeptidase Y antibody. Suitable for WB and reacts with Saccharomyces cerevisiae samples. Cited in 7 publications.

View Alternative Names

YMR297W, PRC1, Carboxypeptidase Y, CPD-Y, cpY, Carboxypeptidase YSCY

1 Images

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Western blot - Anti-Carboxypeptidase Y antibody [10A5B5] (AB113685)

All lanes:

Western blot - Anti-Carboxypeptidase Y antibody [10A5B5] (ab113685)

Lane 1:

Yeast whole cell lysate at 15 µg

Lane 2:

Yeast membrane preparation at 15 µg

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

10A5B5

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Saccharomyces cerevisiae

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Saccharomyces cerevisiae": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5 µg/mL", "WB-species-notes": "<p></p>" } } }

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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification technique

Proprietary technique

Purification notes

Near homogeneity (Purity >95% by SDS-PAGE). The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by chemical fractionation.

Storage buffer

pH: 7.5 Preservative: 0.02% Sodium azide Constituents: HEPES buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Vacuolar serine-type carboxypeptidase involved in degradation of small peptides (PubMed : 8679540). Digests preferentially peptides containing an aliphatic or hydrophobic residue in P1' position, as well as methionine, leucine or phenylalanine in P1 position of ester substrate (PubMed : 8679540). Also plays a role in breakdown of the autophagic body and the autophagosome-dependent protein synthesis (PubMed : 29514932). Plays a key role in phytochelatin (PC) synthesis from glutathione (GSH) by cleaving the Gly from GSH and form the PC-peptides of the structure (gamma-Glu-Cys)2-Gly (PubMed : 17408619). Also involved in resistance to xenobiotics via the degradation of glutathione-S-conjugates (PubMed : 19897216).

See full target information PRC1

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 11:1506 PubMed32198400

2020

The cryo-EM structure of the SNX-BAR Mvp1 tetramer.

Applications

Unspecified application

Species

Unspecified reactive species

Dapeng Sun,Natalia V Varlakhanova,Bryan A Tornabene,Rajesh Ramachandran,Peijun Zhang,Marijn G J Ford

Scientific reports 9:10747 PubMed31341193

2019

Ent3 and GGA adaptors facilitate diverse anterograde and retrograde trafficking events to and from the prevacuolar endosome.

Applications

Unspecified application

Species

Unspecified reactive species

Francisco Yanguas,Esteban Moscoso-Romero,M-Henar Valdivieso

Nature 540:134-138 PubMed27905431

2016

The SND proteins constitute an alternative targeting route to the endoplasmic reticulum.

Applications

Unspecified application

Species

Unspecified reactive species

Naama Aviram,Tslil Ast,Elizabeth A Costa,Eric C Arakel,Silvia G Chuartzman,Calvin H Jan,Sarah Haßdenteufel,Johanna Dudek,Martin Jung,Stefan Schorr,Richard Zimmermann,Blanche Schwappach,Jonathan S Weissman,Maya Schuldiner

Journal of cell science 127:3017-23 PubMed24849653

2014

A cytosolic degradation pathway, prERAD, monitors pre-inserted secretory pathway proteins.

Applications

Species

Saccharomyces cerevisiae S288C

Tslil Ast,Naama Aviram,Silvia Gabriela Chuartzman,Maya Schuldiner

Molecular and cellular biology 33:526-42 PubMed23166297

2012

Saccharomyces cerevisiae Env7 is a novel serine/threonine kinase 16-related protein kinase and negatively regulates organelle fusion at the lysosomal vacuole.

Applications

Unspecified application

Species

Unspecified reactive species

Surya P Manandhar,Florante Ricarte,Stephanie M Cocca,Editte Gharakhanian

The Journal of biological chemistry 287:35709-21 PubMed22915593

2012

A role for iron-sulfur clusters in the regulation of transcription factor Yap5-dependent high iron transcriptional responses in yeast.

Applications

Species

Saccharomyces cerevisiae S288C

Liangtao Li,Ren Miao,Sophie Bertram,Xuan Jia,Diane M Ward,Jerry Kaplan

PloS one 7:e34024 PubMed22529904

2012

Accumulation of an antidepressant in vesiculogenic membranes of yeast cells triggers autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Jingqiu Chen,Daniel Korostyshevsky,Sean Lee,Ethan O Perlstein

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Carboxypeptidase Y antibody [10A5B5]

Western blot - Anti-Carboxypeptidase Y antibody [10A5B5] (AB113685)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (7)

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