Mouse Multiclonal CEACAM5 antibody. Carrier free. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Cell within Human CEACAM5.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carcino Embryonic Antigen CEA antibody [COL-1 + CEA31 + C66/261] - BSA and Azide free (AB212271), expandable thumbnail](https://content.abcam.com/products/images/carcino-embryonic-antigen-cea-antibody-col-1-cea31-c66-261-bsa-and-azide-free-ab212271--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img139582.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carcino Embryonic Antigen CEA antibody [COL-1 + CEA31 + C66/261] - BSA and Azide free (AB212271)")
Key facts
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Multiclonal
Immunogen
- Recombinant Cell within Human CEACAM5. The exact immunogen used to generate this antibody is proprietary information. Database link P06731
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-0.50000 µg/mL | Notes Primary incubation for 30 minutes at RT. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Associated Products
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Target data
Function
Cell surface glycoprotein that plays a role in cell adhesion, intracellular signaling and tumor progression (PubMed:10864933, PubMed:10910050, PubMed:2803308). Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6 (PubMed:2803308). Plays a role as an oncogene by promoting tumor progression; induces resistance to anoikis of colorectal carcinoma cells (PubMed:10910050). (Microbial infection) Receptor for E.coli Dr adhesins. Binding of E.coli Dr adhesins leads to dissociation of the homodimer.
Alternative names
CD66e, CEA, CEACAM5, Cell adhesion molecule CEACAM5, Carcinoembryonic antigen, Carcinoembryonic antigen-related cell adhesion molecule 5, Meconium antigen 100, CEA cell adhesion molecule 5
Recommended products
Mouse Multiclonal CEACAM5 antibody. Carrier free. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Cell within Human CEACAM5.
Key facts
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Multiclonal
- Immunogen
- Recombinant Cell within Human CEACAM5. The exact immunogen used to generate this antibody is proprietary information. Database link P06731
- Carrier free
- Yes
- Clone number
- COL-1 + CEA31 + C66/261
- Purification technique
- Affinity purification Protein A/G
- Concentration
- Purification notes
ab212271 was purified from Bioreactor Concentrate by Protein A/G.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Isotype: IgG2a & IgG1, kappa.
What is an oligoclonal antibody?
This oligoclonal antibody is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CEACAM5 also known as Carcinoembryonic Antigen (CEA) or CD66e is a glycoprotein found mainly on the surface of epithelial cells. This protein has a mass of approximately 180 kDa. It gets expressed in a variety of tissues with high expression in the gastrointestinal tract including the colon and rectum. CEACAM5 is a member of the immunoglobulin superfamily and plays a role in cell adhesion influencing how cells stick to each other.
- Biological function summary
CEACAM5 contributes to cellular processes involving cell adhesion and immune response modulation. This protein operates as part of a larger group of cell adhesion molecules and does not function as part of a complex. CEACAM5 can affect the interactions between cells and the extracellular matrix which has implications for tissue structuring and integrity. It helps the immune system recognize and respond to infectious agents or cellular anomalies.
- Pathways
CEACAM5 involvement extends to immune response regulation and cell adhesion pathways. It interacts functionally with proteins like E-cadherin in these pathways which contribute to maintaining epithelial integrity. These pathways are critical for normal tissue maintenance and immune system function especially in mucosal surfaces where pathogen entry occurs. Understanding CEACAM5 interactions provides insight into cellular communication and adhesion mechanisms.
- Associated diseases and disorders
CEACAM5 is associated with colorectal cancer and lung adenocarcinoma. Its overexpression and release into the bloodstream make it a popular biomarker for these diseases. CEACAM5 is used in clinical applications such as CEA ELISA assays for cancer detection and monitoring. In colorectal cancer CEACAM5 interacts with proteins like CEACAM6 to promote tumor invasiveness and metastasis. Anti-CEACAM5 therapeutics including antibody-drug conjugates (anti-CEACAM5 ADC) are being developed to target and treat associated malignancies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carcino Embryonic Antigen CEA antibody [COL-1 + CEA31 + C66/261] - BSA and Azide free (ab212271), expandable thumbnail](https://content.abcam.com/products/images/carcino-embryonic-antigen-cea-antibody-col-1-cea31-c66-261-bsa-and-azide-free-ab212271--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img139582.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carcino Embryonic Antigen CEA antibody [COL-1 + CEA31 + C66/261] - BSA and Azide free (ab212271)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carcino Embryonic Antigen CEA antibody [COL-1 + CEA31 + C66/261] - BSA and Azide free (ab212271)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon carcinoma tissue labeling Carcino Embryonic Antigen CEA with ab212271 at 0.5 μg/ml.
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