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AB140203

Anti-CARD14 antibody

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(1 Publication)

Rabbit Polyclonal CARD14 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CARD14.

View Alternative Names

CARMA2, CARD14, Caspase recruitment domain-containing protein 14, CARD-containing MAGUK protein 2, Carma 2

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARD14 antibody (AB140203)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARD14 antibody (AB140203)

Immunohistochemical analysis of paraffin-embedded Human placenta tissue labelling CARD14 with ab140203 at 5 µg/ml.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human CARD14. The exact immunogen used to generate this antibody is proprietary information.

Q9BXL6

Specificity

At least three isoforms of CARD14 (CARMA2) are known to exist; this antibody will only detect isoform 1. ab140203 is predicted not to cross-react with other CARMA proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CARD14 also known as CARMA2 is a protein involved in the regulation of immune responses. Its molecular weight is approximately 120 kDa. This protein is expressed mainly in keratinocytes which are cells found in the epidermis of the skin. Mechanically CARD14 functions as a scaffold protein that facilitates the assembly of signalosomes. These structures are essential for transmitting important signals within cells that activate various pathways linked to inflammation and immune responses.
Biological function summary

CARD14 plays a significant role in the NF-kB signaling pathway activating it to promote inflammation and immune responses. CARD14 forms a complex with other proteins to facilitate the activation of this pathway which leads to the expression of genes involved in inflammation and immune responses. Through this complex formation CARD14 helps modulate both the innate and adaptive immune systems by influencing the behavior of immune cells making it critical for proper skin and immune function.

Pathways

CARD14 acts as a mediator within the NF-kB and MAPK pathways both vital for cellular responses to stress cytokines and pathogens. CARD14 interacts with other proteins such as BCL10 and MALT1 which are part of the CARD-CC/BCL10/MALT1 signalosome. This interaction helps transduce signals that promote the expression of pro-inflammatory cytokines and chemokines which are critical for mounting effective immune reactions.

CARD14 has strong connections to psoriasis vulgaris and pityriasis rubra pilaris. Mutations in the CARD14 gene are associated with these inflammatory skin conditions which are marked by excessive immune activation and chronic inflammation. CARD14 influences these diseases by affecting the expression of cytokines mediated through the NF-kB pathway. Its interaction with BCL10 further emphasizes its role in promoting inflammation contributing to the pathogenesis of these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a scaffolding protein that can activate the inflammatory transcription factor NF-kappa-B and p38/JNK MAP kinase signaling pathways. Forms a signaling complex with BCL10 and MALT1, and activates MALT1 proteolytic activity and inflammatory gene expression. MALT1 is indispensable for CARD14-induced activation of NF-kappa-B and p38/JNK MAP kinases (PubMed : 11278692, PubMed : 21302310, PubMed : 27071417, PubMed : 27113748). May play a role in signaling mediated by TRAF2, TRAF3 and TRAF6 and protects cells against apoptosis.. Isoform 3. Not able to activate the inflammatory transcription factor NF-kappa-B and may function as a dominant negative regulator (PubMed : 21302310, PubMed : 26358359).
See full target information CARD14
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology reports 46: PubMed34278501

2021

Mechanism of tumor‑derived extracellular vesicles in regulating renal cell carcinoma progression by the delivery of MALAT1.

Applications

Unspecified application

Species

Unspecified reactive species

Chengluo Jin,Linmei Shi,Kunlun Li,Wei Liu,Yu Qiu,Yakun Zhao,Bai Zhao,Zhexun Li,Yifei Li,Qingguo Zhu
View all publications
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Product promise

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