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AB168096

Anti-CARD14 antibody

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(2 Publications)

Mouse Polyclonal CARD14 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CARD14.

View Alternative Names

CARMA2, CARD14, Caspase recruitment domain-containing protein 14, CARD-containing MAGUK protein 2, Carma 2

1 Images
Western blot - Anti-CARD14 antibody (AB168096)
  • WB

Unknown

Western blot - Anti-CARD14 antibody (AB168096)

All lanes:

Western blot - Anti-CARD14 antibody (ab168096) at 1 µg/mL

Lane 1:

CARD14 transfected 293T cell line lysate at 15 µL

Lane 2:

Non-transfected 293T cell line lysate at 15 µL

Predicted band size: 113 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CARD14.

Q9BXL6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CARD14 also known as CARMA2 is a protein involved in the regulation of immune responses. Its molecular weight is approximately 120 kDa. This protein is expressed mainly in keratinocytes which are cells found in the epidermis of the skin. Mechanically CARD14 functions as a scaffold protein that facilitates the assembly of signalosomes. These structures are essential for transmitting important signals within cells that activate various pathways linked to inflammation and immune responses.
Biological function summary

CARD14 plays a significant role in the NF-kB signaling pathway activating it to promote inflammation and immune responses. CARD14 forms a complex with other proteins to facilitate the activation of this pathway which leads to the expression of genes involved in inflammation and immune responses. Through this complex formation CARD14 helps modulate both the innate and adaptive immune systems by influencing the behavior of immune cells making it critical for proper skin and immune function.

Pathways

CARD14 acts as a mediator within the NF-kB and MAPK pathways both vital for cellular responses to stress cytokines and pathogens. CARD14 interacts with other proteins such as BCL10 and MALT1 which are part of the CARD-CC/BCL10/MALT1 signalosome. This interaction helps transduce signals that promote the expression of pro-inflammatory cytokines and chemokines which are critical for mounting effective immune reactions.

CARD14 has strong connections to psoriasis vulgaris and pityriasis rubra pilaris. Mutations in the CARD14 gene are associated with these inflammatory skin conditions which are marked by excessive immune activation and chronic inflammation. CARD14 influences these diseases by affecting the expression of cytokines mediated through the NF-kB pathway. Its interaction with BCL10 further emphasizes its role in promoting inflammation contributing to the pathogenesis of these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a scaffolding protein that can activate the inflammatory transcription factor NF-kappa-B and p38/JNK MAP kinase signaling pathways. Forms a signaling complex with BCL10 and MALT1, and activates MALT1 proteolytic activity and inflammatory gene expression. MALT1 is indispensable for CARD14-induced activation of NF-kappa-B and p38/JNK MAP kinases (PubMed : 11278692, PubMed : 21302310, PubMed : 27071417, PubMed : 27113748). May play a role in signaling mediated by TRAF2, TRAF3 and TRAF6 and protects cells against apoptosis.. Isoform 3. Not able to activate the inflammatory transcription factor NF-kappa-B and may function as a dominant negative regulator (PubMed : 21302310, PubMed : 26358359).
See full target information CARD14
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 43:114589 PubMed39110589

2024

Regulation of MYC by CARD14 in human epithelium is a determinant of epidermal homeostasis and disease.

Applications

Unspecified application

Species

Unspecified reactive species

Stanley B DeVore,Matthew Schuetz,Lauren Alvey,Henry Lujan,David E Ochayon,Lindsey Williams,Wan Chi Chang,Alyssa Filuta,Brandy Ruff,Arjun Kothari,Jennifer M Hahn,Eric Brandt,Latha Satish,Krishna Roskin,Andrew B Herr,Jocelyn M Biagini,Lisa J Martin,Deniz Cagdas,Sevgi Keles,Joshua D Milner,Dorothy M Supp,Gurjit K Khurana Hershey

The Journal of pathology 251:365-377 PubMed32418203

2020

Malignant melanotic Xp11 neoplasms exhibit a clinicopathologic spectrum and gene expression profiling akin to alveolar soft part sarcoma: a proposal for reclassification.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Tong Wang,Ru Fang,Ru-Song Zhang,Sheng-Bing Ye,Rui Li,Xuan Wang,Rui Pan,Chong Liu,Jie-Yu Chen,Ming Zhao,Xiao-Dong Teng,Wen-Juan Yu,Yu-Jun Li,Feng-Hua Wang,Jian-Guo Zhang,Qi-Chang Yang,Yong-Sheng Zhang,Zhen-Feng Lu,Heng-Hui Ma,Xiao-Jun Zhou,Qiu-Yuan Xia,Qiu Rao
View all publications
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