AB151560

Anti-CARD6 antibody [EPR9718]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal CARD6 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

View Alternative Names

Caspase recruitment domain-containing protein 6, CARD6

2 Images

Flow Cytometry (Intracellular) - Anti-CARD6 antibody [EPR9718] (AB151560)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-CARD6 antibody [EPR9718] (AB151560)

Intracellular flow cytometric analysis of permeabilized HeLa cells labeling CARD6 with ab151560 at 1/10 dilution (red), compared to a Rabbit IgG control antibody (green).

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Western blot - Anti-CARD6 antibody [EPR9718] (AB151560)

All lanes:

Western blot - Anti-CARD6 antibody [EPR9718] (ab151560) at 1/1000 dilution

Lane 1:

MCF7 lysate at 10 µg

Lane 2:

HeLa lysate at 10 µg

Lane 3:

Human fetal brain lysate at 10 µg

Lane 4:

293T lysate at 10 µg

Lane 5:

Human fetal kidney lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 116 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9718

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CARD6 also known as Caspase Recruitment Domain-Containing Protein 6 is a protein that weighs approximately 104 kDa. The protein is identified for its involvement in the immune response and connects with microtubules in the cell. It is expressed in various tissues including the heart brain and skeletal muscles. CARD6 contains a CARD (Caspase Activation and Recruitment Domain) enabling it to interact with other proteins containing similar domains.

Biological function summary

CARD6 influences immune system regulation playing a role in apoptosis and inflammation. It does not form a complex with other proteins but instead interacts directly with several proteins through its CARD domain. Notably CARD6 modulates the functions of NF-kB a transcription factor important for immune response regulation and cellular stress responses.

Pathways

CARD6 participates in the NF-kB signaling pathway and the interferon regulatory pathway. These pathways are essential for mediating immune responses and controlling inflammation within the body. CARD6 shows interaction with proteins like RIP2 and TRAF6 within these pathways which further affect the NF-kB signaling cascade.

CARD6 has been linked to Crohn's disease and certain cancers. Abnormalities in its function or expression can lead to dysregulated NF-kB activity contributing to these conditions. CARD6 interacts with proteins such as RIP2 in autoimmune disorders like Crohn's disease and its altered signaling pathway connections can play a role in cancer progression by affecting cell survival and proliferation mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May be involved in apoptosis.

See full target information CARD6

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Exploration (Beijing, China) 5:e20230178 PubMed40873646

2025

Enhanced Blood-Brain Barrier Penetrability of BACE1 SiRNA-Loaded Prussian Blue Nanocomplexes for Alzheimer's Disease Synergy Therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyuan Ding,Yanyu Hu,Xiaotong Feng,Zekun Wang,Qile Song,Chunxue Dai,Bangjia Yang,Xiaoyan Fu,Dongdong Sun,Cundong Fan

Aging 11:12213-12235 PubMed31841440

2019

Targeting CARD6 attenuates spinal cord injury (SCI) in mice through inhibiting apoptosis, inflammation and oxidative stress associated ROS production.

Applications

Unspecified application

Species

Unspecified reactive species

Jiang Lin Wang,Xiao Luo,Li Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CARD6 antibody [EPR9718]

Flow Cytometry (Intracellular) - Anti-CARD6 antibody [EPR9718] (AB151560)

Western blot - Anti-CARD6 antibody [EPR9718] (AB151560)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise