AB10231

Anti-Cardiac Troponin I antibody [4C2]

Name: Anti-Cardiac Troponin I antibody [4C2] Mouse monoclonal (ab10231) | Abcam
Brand: Abcam Limited
SKU: AB10231
Price: 450 USD
Availability: InStock
Rating: 5 (3 reviews)

(3 Reviews)

(28 Publications)

Mouse Monoclonal Cardiac Troponin I antibody. Suitable for IHC-P and reacts with Human samples. Cited in 28 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human TNNI3.

View Alternative Names

TNNC1, TNNI3, Cardiac troponin I

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [4C2] (AB10231)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [4C2] (AB10231)

ab10231 staining human heart tissue. Staining is localised to cytoplasm.
Left panel : with primary antibody at 1ug/ml. Right panel : isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

4C2

Isotype

IgG2a

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Native Full Length Protein corresponding to Human TNNI3.

P19429

Epitope

23-29 aa

Specificity

reacts with free cardiac troponin I (cTnI) and cTnI forming complexes with other troponin components (in the presence of 5 mM EDTA). Not affected by heparin, phosphorylation, oxidation and troponin complex formation. Does not cross-react with skeletal muscle troponin I.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": "<p></p>" } } }

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Product details

Concentration varies from lot to lot and can be provided on request.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Purity tested by electrophoresis.

Storage buffer

pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

See full target information TNNI3

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Publications (28)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:5902 PubMed40593704

2025

Generation of mature epicardium derived from human-induced pluripotent stem cells via inhibition of mTOR signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Tian,Antonio Lucena-Cacace,Kanae Tani,Amanda Putri Elvandari,Rodolfo S Allendes Osorio,Megumi Narita,Yasuko Matsumura,Ian Costa Paixao,Yutaro Miyoshi,Azusa Inagaki,Julia Junghof,Yoshinori Yoshida

PloS one 19:e0295131 PubMed38446773

2024

Systematic cryopreservation study of cardiac myoblasts in suspension.

Applications

Unspecified application

Species

Unspecified reactive species

Elham Ashrafi,Milica Radisic,Janet A W Elliott

Current protocols 3:e889 PubMed37747346

2023

Speckle-Tracking Strain Analysis for Mapping Spatiotemporal Contractility of Induced Pluripotent Stem Cell (iPSC)-Derived Cardiomyocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Mohammad Izadifar,Tunde Berecz,Biao Li,Jean Kitty Kit Yee Tang,Gabor Foldes,Agota Apati,Andras Nagy

Journal of personalized medicine 12: PubMed35207702

2022

A New Versatile Platform for Assessment of Improved Cardiac Performance in Human-Engineered Heart Tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Marcelo C Ribeiro,José M Rivera-Arbeláez,Carla Cofiño-Fabres,Verena Schwach,Rolf H Slaats,Simone A Ten Den,Kim Vermeul,Albert van den Berg,José M Pérez-Pomares,Loes I Segerink,Juan A Guadix,Robert Passier

Bioelectrochemistry (Amsterdam, Netherlands) 144:107981 PubMed34847374

2021

Reduction of plasmid vector backbone length enhances reporter gene expression.

Applications

Unspecified application

Species

Unspecified reactive species

Carly Boye,Sezgi Arpag,Michael Francis,Scott DeClemente,Aislin West,Richard Heller,Anna Bulysheva

Bioelectrochemistry (Amsterdam, Netherlands) 142:107892 PubMed34371349

2021

Cardioporation enhances myocardial gene expression in rat heart.

Applications

Unspecified application

Species

Unspecified reactive species

Carly Boye,Sezgi Arpag,Nina Burcus,Cathryn Lundberg,Scott DeClemente,Richard Heller,Michael Francis,Anna Bulysheva

Journal of molecular and cellular cardiology 150:77-90 PubMed33148509

2020

Mutation location of HCM-causing troponin T mutations defines the degree of myofilament dysfunction in human cardiomyocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Maike Schuldt,Jamie R Johnston,Huan He,Roy Huurman,Jiayi Pei,Magdalena Harakalova,Corrado Poggesi,Michelle Michels,Diederik W D Kuster,Jose R Pinto,Jolanda van der Velden

Basic research in cardiology 115:60 PubMed32910221

2020

Deletion of obscurin immunoglobulin domains Ig58/59 leads to age-dependent cardiac remodeling and arrhythmia.

Applications

Unspecified application

Species

Unspecified reactive species

Alyssa Grogan,Andrew Coleman,Humberto Joca,Henk Granzier,Mark W Russel,Christopher W Ward,Aikaterini Kontrogianni-Konstantopoulos

Frontiers in immunology 10:2774 PubMed31866998

2019

Modeling Cardiac Dysfunction Following Traumatic Hemorrhage Injury: Impact on Myocardial Integrity.

Applications

Unspecified application

Species

Unspecified reactive species

Johanna Wall,Sriveena Naganathar,Banjerd Praditsuktavorn,Oscar F Bugg,Simon McArthur,Christoph Thiemermann,Jordi L Tremoleda,Karim Brohi

Nanomedicine (London, England) 15:163-181 PubMed31799897

2019

miRNA transfection via poly(amidoamine)-based delivery vector prevents hypoxia/reperfusion-induced cardiomyocyte apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Nida Sayed,Prajakta Tambe,Pramod Kumar,Sachin Jadhav,Kishore M Paknikar,Virendra Gajbhiye

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cardiac Troponin I antibody [4C2]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [4C2] (AB10231)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Epitope

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (28)

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