Mouse Monoclonal Cardiac Troponin I antibody. Suitable for IHC-P and reacts with Human samples. Cited in 25 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human TNNI3.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [4C2] (AB10231), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-i-antibody-4c2-ab10231--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img20843.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [4C2] (AB10231)")
Publications
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- Current protocols 3:e8892023Speckle-Tracking Strain Analysis for Mapping Spatiotemporal Contractility of Induced Pluripotent Stem Cell (iPSC)-Derived Cardiomyocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMohammad Izadifar,Tunde Berecz,Biao Li,Jean Kitty Kit Yee Tang,Gabor Foldes,Agota Apati,Andras NagyPubMed 37747346
- Journal of personalized medicine 12:2022A New Versatile Platform for Assessment of Improved Cardiac Performance in Human-Engineered Heart Tissues.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarcelo C Ribeiro,José M Rivera-Arbeláez,Carla Cofiño-Fabres,Verena Schwach,Rolf H Slaats,Simone A Ten Den,Kim Vermeul,Albert van den Berg,José M Pérez-Pomares,Loes I Segerink,Juan A Guadix,Robert PassierPubMed 35207702
- Bioelectrochemistry (Amsterdam, Netherlands) 144:1079812021Reduction of plasmid vector backbone length enhances reporter gene expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCarly Boye,Sezgi Arpag,Michael Francis,Scott DeClemente,Aislin West,Richard Heller,Anna BulyshevaPubMed 34847374
- Bioelectrochemistry (Amsterdam, Netherlands) 142:1078922021Cardioporation enhances myocardial gene expression in rat heart.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCarly Boye,Sezgi Arpag,Nina Burcus,Cathryn Lundberg,Scott DeClemente,Richard Heller,Michael Francis,Anna BulyshevaPubMed 34371349
- Journal of molecular and cellular cardiology 150:77-902020Mutation location of HCM-causing troponin T mutations defines the degree of myofilament dysfunction in human cardiomyocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaike Schuldt,Jamie R Johnston,Huan He,Roy Huurman,Jiayi Pei,Magdalena Harakalova,Corrado Poggesi,Michelle Michels,Diederik W D Kuster,Jose R Pinto,Jolanda van der VeldenPubMed 33148509
- Frontiers in immunology 10:27742019Modeling Cardiac Dysfunction Following Traumatic Hemorrhage Injury: Impact on Myocardial Integrity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJohanna Wall et. al.PubMed 31866998
- Nanomedicine (London, England) 15:163-1812019miRNA transfection via poly(amidoamine)-based delivery vector prevents hypoxia/reperfusion-induced cardiomyocyte apoptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNida Sayed,Prajakta Tambe,Pramod Kumar,Sachin Jadhav,Kishore M Paknikar,Virendra GajbhiyePubMed 31799897
- The Analyst 144:6533-65402019SERS-based immunoassay using gold-patterned array chips for rapid and sensitive detection of dual cardiac biomarkers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZiyi Cheng,Rui Wang,Yanlong Xing,Linlu Zhao,Jaebum Choo,Fabiao YuPubMed 31553332
- Experimental animals 69:54-612019Evaluation of cardiac troponin I in serum and myocardium of rabbits with experimentally induced polymicrobial sepsis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKenan Çağrı Tümer,Haydar Özdemİr,Hatice EröksüzPubMed 31462610
- Journal of applied biomedicine 17:392019A cardiac troponin I study in a minimally invasive myocardial infarction canine model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesReyaj Mikrani,Cuilan Liang,Muhammad Naveed,Asghar Ali Kamboh,Muhammad Abbas,Birendra Chaurasiya,Li Xue,Zhou XiaohuiPubMed 34907744
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Native Full Length Protein corresponding to Human TNNI3. Database link P19429
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Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
Associated Products
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Target data
Function
Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
Alternative names
TNNC1, TNNI3, Cardiac troponin I
Recommended products
Mouse Monoclonal Cardiac Troponin I antibody. Suitable for IHC-P and reacts with Human samples. Cited in 25 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human TNNI3.
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Native Full Length Protein corresponding to Human TNNI3. Database link P19429
- Clone number
- 4C2
- Purification technique
- Affinity purification Protein A
- Specificity
reacts with free cardiac troponin I (cTnI) and cTnI forming complexes with other troponin components (in the presence of 5 mM EDTA).
Not affected by heparin, phosphorylation, oxidation and troponin complex formation.
Does not cross-react with skeletal muscle troponin I.- Epitope
- 23-29 aa
- Concentration
- Purification notes
Purity tested by electrophoresis.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Concentration varies from lot to lot and can be provided on request.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [4C2] (ab10231), expandable thumbnail](https://content.abcam.com/products/images/cardiac-troponin-i-antibody-4c2-ab10231--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img20843.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [4C2] (ab10231)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [4C2] (ab10231)
ab10231 staining human heart tissue. Staining is localised to cytoplasm.
Left panel: with primary antibody at 1ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
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