AB155047

Anti-Cardiac Troponin I antibody

Name: Anti-Cardiac Troponin I antibody (ab155047) | Abcam
Brand: Abcam Limited
SKU: AB155047
Price: 545 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal Cardiac Troponin I antibody. Suitable for WB, IHC-P and reacts with Rat, Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TNNI3 aa 1 to C-terminus.

View Alternative Names

TNNC1, TNNI3, Cardiac troponin I

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody (AB155047)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody (AB155047)

Immunohistochemical analysis of paraffin embedded mouse heart tissue stained for Troponin I (cardiac) protein at cytoplasm with ab155047 at a 1/250 dilution. beta Actin, a cytoskeleton marker, stained by beta Actin antibody used at 1/500 dilution. DAPI was used for nuclear staining.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody (AB155047)

Immunohistochemical analysis of paraffin embedded mouse heart tissue stained for Cardiac Troponin I protein at cytosol with ab155047 at a 1/500 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody (AB155047)

Immunohistochemical analysis of paraffin embedded rat heart tissue stained for Troponin I (cardiac) protein at cytosol with ab155047 at a 1/500 dilution.

Antigen retrieval : Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Supplier Data

Western blot - Anti-Cardiac Troponin I antibody (AB155047)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-Cardiac Troponin I antibody (ab155047) at 1/3000 dilution

Lane 1:

Mouse heart tissue lysates at 50 µg

Lane 2:

Rat heart tissue lysates at 50 µg

Secondary

All lanes:

HRP conjugated anti rabbit IgG antibody

Predicted band size: 24 kDa

false

Supplier Data

Western blot - Anti-Cardiac Troponin I antibody (AB155047)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-Cardiac Troponin I antibody (ab155047) at 1/3000 dilution

All lanes:

Mouse heart tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 24 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human TNNI3 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P19429

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

See full target information TNNI3

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of the American Heart Association 11:e027004 PubMed36370010

2022

SENP1 Protects Against Pressure Overload-Induced Cardiac Remodeling and Dysfunction Via Inhibiting STAT3 Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Yang,Di Fan,Zhen Guo,Fang-Yuan Liu,Ming-Yu Wang,Peng An,Zheng Yang,Qi-Zhu Tang

Oncotarget 8:72235-72249 PubMed29069783

2017

TLE1 inhibits anoikis and promotes tumorigenicity in human lung cancer cells through ZEB1-mediated E-cadherin repression.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Yao,Tri Pham,Brandi Temple,Selena Gray,Cornita Cannon,Camry Hardy,Kamari Fletcher,Shubha Kale Ireland,Ahamed Hossain,Renwei Chen,Asim B Abdel-Mageed,Hector Biliran

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cardiac Troponin I antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody (AB155047)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody (AB155047)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody (AB155047)

Western blot - Anti-Cardiac Troponin I antibody (AB155047)

Western blot - Anti-Cardiac Troponin I antibody (AB155047)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (2)

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